Isolation and Characterization of Monoclonal Antibody Directed Against Bovine as2-Casein CECILIA T. LEUNG,1 KONRAD M. KUZMANOFF, and CRAIG W. BEATTIE SpecIalized center for Canoer Research and Education University of Ininols School of Medicine at Chicago Chicago 60612 ABSTRACT

A monoclonal antibody 62-IA of isotype IgM, directed against bovine ~­ casein, was isolated and characterized. Monoclonal antibody 62-IA recognized bovine ~-IIP- and ~-9P-caseins by indirect solid phase radioimmunoassay and Western blot analysis. Crossreactivity toward native genetic variants (A, B, and C) of CXsI-casein was similar but lower (-60 to 70%) than 'that for

Isolation and characterization of monoclonal antibody directed against bovine alpha s2-casein.

A monoclonal antibody 62-1A of isotype IgM, directed against bovine alpha s2-casein, was isolated and characterized. Monoclonal antibody 62-1A recogni...
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