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Intranasal Administration of Combination of Choline Chloride, Vitamin C and Selenium Attenuated The Allergic Effect in Mouse Model of Airway Disease Preeti Bansal, Sanjay Saw, Dhanapal Govindaraj, Naveen Arora

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S0891-5849(14)00234-2 http://dx.doi.org/10.1016/j.freeradbiomed.2014.05.018 FRB12027

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Free Radical Biology and Medicine

Received date: 24 August 2013 Revised date: 1 May 2014 Accepted date: 7 May 2014 Cite this article as: Preeti Bansal, Sanjay Saw, Dhanapal Govindaraj, Naveen Arora, Intranasal Administration of Combination of Choline Chloride, Vitamin C and Selenium Attenuated The Allergic Effect in Mouse Model of Airway Disease, Free Radical Biology and Medicine, http://dx.doi.org/10.1016/j.freeradbiomed.2014.05.018 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Intranasal Administration of Combination of Choline Chloride, Vitamin C and Selenium Attenuated The Allergic Effect in Mouse Model of Airway Disease Preeti Bansal a, b, Sanjay Saw a, b, Dhanapal Govindaraj a, Naveen Arora a* a

Allergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology,

Delhi, India. b

Department of Biotechnology, University of Pune, Ganeshkhind, Pune 411 007, India Corresponding Address: Naveen Arora, Ph.D. Room 509, Allergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi-110007, INDIA Tel: (011) 27666157, Fax: (011) 27667471 Email: [email protected]

Abstract

Respiratory allergic disease is an inflammatory condition accompanied with oxidative stress. Supplementation of an anti-inflammatory agent with antioxidants may have therapeutic effect. In the present study, the effect of choline chloride in combination with antioxidants was evaluated via intranasal route in mice model of allergic airway disease. Balb/c mice were sensitized on days 0, 7th, 14th, challenged on 25 -30 days and a booster challenge on day 38th with cockroach extract (CE). They were treated with choline chloride (ChCl) (1mg/Kg), vitamin C (Vit C) (308.33mg/Kg), selenium (Se) (1mg/Kg) alone or in combination via intranasal route on 31st, 33rd, 35th, 37th, and 39th day. Mice were sacrificed on day 40th to collect blood, bronchoalveolar lavage fluid (BALF), lungs and spleen. Mice immunized with CE showed a significant increase in airway hyper responsiveness (AHR), lung inflammation, Th2 cytokines and oxidative stress markers intracellular reactive oxygen species (iROS) and 8-isoprostanes as compared to PBS



1

control group. A significant decrease was observed in these parameters with all the treatments (p

Intranasal administration of a combination of choline chloride, vitamin C, and selenium attenuates the allergic effect in a mouse model of airway disease.

Respiratory allergic disease is an inflammatory condition accompanied by oxidative stress. Supplementation of an anti-inflammatory agent with antioxid...
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