7330
Biochemistry 1991, 30, 7330-7340
Interfacial Catalysis by Phospholipase A2: Monomeric Enzyme Is Fully Catalytically Active at the Bilayer Interface? Mahendra Kumar Jain,*.* Girish Ranadive,* Bao-Zhu Yu,* and H. M. Verheijs Department of Chemistry, University of Delaware, Newark, Delaware 19716, and Department of Biochemistry, State University, Utrecht, The Netherlands Received September 17, 1990; Revised Manuscript Received March 26, 1991
Interfacial catalysis in the scooting mode by phospholipase A2 (PLA2) from pancreas and venoms ( 18 different preparations) was examined on vesicles of 1,2-dimyristoyl-sn-glycero-3-phosphomethanolunder the conditions where the rates of transbilayer and intervesicle exchanges of the enzyme, substrate, and the products of hydrolysis were negligible on the time scale (
Biochemistry 1991, 30, 7330-7340
Interfacial Catalysis by Phospholipase A2: Monomeric Enzyme Is Fully Catalytically Active at the Bilayer Interface? Mahendra Kumar Jain,*.* Girish Ranadive,* Bao-Zhu Yu,* and H. M. Verheijs Department of Chemistry, University of Delaware, Newark, Delaware 19716, and Department of Biochemistry, State University, Utrecht, The Netherlands Received September 17, 1990; Revised Manuscript Received March 26, 1991
Interfacial catalysis in the scooting mode by phospholipase A2 (PLA2) from pancreas and venoms ( 18 different preparations) was examined on vesicles of 1,2-dimyristoyl-sn-glycero-3-phosphomethanolunder the conditions where the rates of transbilayer and intervesicle exchanges of the enzyme, substrate, and the products of hydrolysis were negligible on the time scale (
