www.nature.com/scientificreports
OPEN
received: 26 November 2015 accepted: 31 March 2016 Published: 15 April 2016
Insulin resistance is associated with altered amino acid metabolism and adipose tissue dysfunction in normoglycemic women Petri Wiklund1,2, Xiaobo Zhang1, Satu Pekkala2, Reija Autio3, Lingjia Kong4, Yifan Yang1, Sirkka Keinänen-Kiukaanniemi5, Markku Alen5,6 & Sulin Cheng1,2 Insulin resistance is associated adiposity, but the mechanisms are not fully understood. In this study, we aimed to identify early metabolic alterations associated with insulin resistance in normoglycemic women with varying degree of adiposity. One-hundred and ten young and middle-aged women were divided into low and high IR groups based on their median HOMA-IR (0.9 ± 0.4 vs. 2.8 ± 1.2). Body composition was assessed using DXA, skeletal muscle and liver fat by proton magnetic resonance spectroscopy, serum metabolites by nuclear magnetic resonance spectroscopy and adipose tissue and skeletal muscle gene expression by microarrays. High HOMA-IR subjects had higher serum branched-chain amino acid concentrations (BCAA) (p
OPEN
received: 26 November 2015 accepted: 31 March 2016 Published: 15 April 2016
Insulin resistance is associated with altered amino acid metabolism and adipose tissue dysfunction in normoglycemic women Petri Wiklund1,2, Xiaobo Zhang1, Satu Pekkala2, Reija Autio3, Lingjia Kong4, Yifan Yang1, Sirkka Keinänen-Kiukaanniemi5, Markku Alen5,6 & Sulin Cheng1,2 Insulin resistance is associated adiposity, but the mechanisms are not fully understood. In this study, we aimed to identify early metabolic alterations associated with insulin resistance in normoglycemic women with varying degree of adiposity. One-hundred and ten young and middle-aged women were divided into low and high IR groups based on their median HOMA-IR (0.9 ± 0.4 vs. 2.8 ± 1.2). Body composition was assessed using DXA, skeletal muscle and liver fat by proton magnetic resonance spectroscopy, serum metabolites by nuclear magnetic resonance spectroscopy and adipose tissue and skeletal muscle gene expression by microarrays. High HOMA-IR subjects had higher serum branched-chain amino acid concentrations (BCAA) (p
