RESEARCH ARTICLE
Inhibition of ROCK1 kinase modulates both tumor cells and stromal fibroblasts in pancreatic cancer Clifford J. Whatcott1, Serina Ng1, Michael T. Barrett2, Galen Hostetter3, Daniel D. Von Hoff1, Haiyong Han1*
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1 Molecular Medicine Division, The Translational Genomics Research Institute, Phoenix, Arizona, United States of America, 2 Mayo Clinic Cancer Center, Scottsdale, Arizona, United States of America, 3 Laboratory of Analytical Pathology, The Van Andel Research Institute, Grand Rapids, MI, United States of America * [email protected]
Abstract OPEN ACCESS Citation: Whatcott CJ, Ng S, Barrett MT, Hostetter G, Von Hoff DD, Han H (2017) Inhibition of ROCK1 kinase modulates both tumor cells and stromal fibroblasts in pancreatic cancer. PLoS ONE 12(8): e0183871. https://doi.org/10.1371/journal. pone.0183871 Editor: Aamir Ahmad, University of South Alabama Mitchell Cancer Institute, UNITED STATES Received: April 7, 2017 Accepted: August 11, 2017 Published: August 25, 2017 Copyright: © 2017 Whatcott et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported in part by a R01 (CA169281) grant and a U01 (CA128454) grant from the NIH/NCI, a grant from the National Foundation for Cancer Research, and a Stand Up To Cancer-Cancer Research UK-Lustgarten Foundation Pancreatic Cancer Dream Team Research Grant (Grant Number: SU2C-AACR-DT20-16). Stand Up To Cancer is a program of the
ROCK, or Rho-associated coiled coil-containing protein kinase, is a member of the AGC kinase family and has been shown to play a role in cell migration, ECM synthesis, stressfiber assembly, and cell contraction. Increased ROCK expression has been reported in multiple pathological conditions, including cancer. Here, we report increased expression of ROCK 1 in pancreatic tumor epithelial cells as well as in cancer associated fibroblasts (CAF). In our analysis, 62% of tumor samples exhibited 2+ in staining intensity by IHC analysis, versus 40% of adjacent normal tissue samples (P
Inhibition of ROCK1 kinase modulates both tumor cells and stromal fibroblasts in pancreatic cancer Clifford J. Whatcott1, Serina Ng1, Michael T. Barrett2, Galen Hostetter3, Daniel D. Von Hoff1, Haiyong Han1*
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1 Molecular Medicine Division, The Translational Genomics Research Institute, Phoenix, Arizona, United States of America, 2 Mayo Clinic Cancer Center, Scottsdale, Arizona, United States of America, 3 Laboratory of Analytical Pathology, The Van Andel Research Institute, Grand Rapids, MI, United States of America * [email protected]
Abstract OPEN ACCESS Citation: Whatcott CJ, Ng S, Barrett MT, Hostetter G, Von Hoff DD, Han H (2017) Inhibition of ROCK1 kinase modulates both tumor cells and stromal fibroblasts in pancreatic cancer. PLoS ONE 12(8): e0183871. https://doi.org/10.1371/journal. pone.0183871 Editor: Aamir Ahmad, University of South Alabama Mitchell Cancer Institute, UNITED STATES Received: April 7, 2017 Accepted: August 11, 2017 Published: August 25, 2017 Copyright: © 2017 Whatcott et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported in part by a R01 (CA169281) grant and a U01 (CA128454) grant from the NIH/NCI, a grant from the National Foundation for Cancer Research, and a Stand Up To Cancer-Cancer Research UK-Lustgarten Foundation Pancreatic Cancer Dream Team Research Grant (Grant Number: SU2C-AACR-DT20-16). Stand Up To Cancer is a program of the
ROCK, or Rho-associated coiled coil-containing protein kinase, is a member of the AGC kinase family and has been shown to play a role in cell migration, ECM synthesis, stressfiber assembly, and cell contraction. Increased ROCK expression has been reported in multiple pathological conditions, including cancer. Here, we report increased expression of ROCK 1 in pancreatic tumor epithelial cells as well as in cancer associated fibroblasts (CAF). In our analysis, 62% of tumor samples exhibited 2+ in staining intensity by IHC analysis, versus 40% of adjacent normal tissue samples (P
