Clin Rheumatol DOI 10.1007/s10067-015-2947-5
BRIEF REPORT
Increased expression of IL-28RA mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus Yu-Yan Cheng 1 & Yu-Jun Sheng 1 & Yan Chang 1 & Yan Lin 1 & Zheng-Wei Zhu 1 & Lei-Lei Wen 1 & Chao Yang 1 & Lu Liu 1 & Lu-Lu Yang 1 & Fu-Sheng Zhou 1 & Xiao-Dong Zheng 1 & Xian-Yong Yin 1 & Sheng-Quan Zhang 1 & Yong Cui 2 & Sen Yang 1 & Xue-Jun Zhang 1
Received: 11 February 2015 / Revised: 26 March 2015 / Accepted: 17 April 2015 # International League of Associations for Rheumatology (ILAR) 2015
Abstract Systemic lupus erythematosus (SLE) is a systemic autoimmune and inflammatory disease with a strong genetic contribution and characterized by kinds of immune reactions. Our previous genome-wide association studies have identified IL-28RA as a susceptibility gene for SLE. In this study, we performed a quantitative reverse transcription polymerase chain reaction (RT-PCR) in 62 patients with SLE and 69 controls to investigate the different expression levels of IL-28RA in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy controls and the association between IL28RA expression and systemic lupus erythematosus disease activity index (SLEDAI) or the variant of the singlenucleotide polymorphism (SNP) rs4649203. The expression levels of IL-28RA messenger RNA (mRNA) in SLE patients were significantly increased compared with those of healthy controls. In addition, there were also significant differences in the expression levels of IL-28RA between active (SLEDAI≥6) or inactive (SLEDAI0.05), separately. Genotypes were compared using the independent-samples t test. Spearman’s correlation coefficient
Clin Rheumatol
rank test was used to assess the association between IL-28RA mRNA expression levels and the SLEDAI scores. Analysis was performed with Statistical Package for the Social Science (SPSS) version 20.0. (SPSS Inc., Chicago, IL, USA). Data were expressed as mean±standard deviation. A P value of
BRIEF REPORT
Increased expression of IL-28RA mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus Yu-Yan Cheng 1 & Yu-Jun Sheng 1 & Yan Chang 1 & Yan Lin 1 & Zheng-Wei Zhu 1 & Lei-Lei Wen 1 & Chao Yang 1 & Lu Liu 1 & Lu-Lu Yang 1 & Fu-Sheng Zhou 1 & Xiao-Dong Zheng 1 & Xian-Yong Yin 1 & Sheng-Quan Zhang 1 & Yong Cui 2 & Sen Yang 1 & Xue-Jun Zhang 1
Received: 11 February 2015 / Revised: 26 March 2015 / Accepted: 17 April 2015 # International League of Associations for Rheumatology (ILAR) 2015
Abstract Systemic lupus erythematosus (SLE) is a systemic autoimmune and inflammatory disease with a strong genetic contribution and characterized by kinds of immune reactions. Our previous genome-wide association studies have identified IL-28RA as a susceptibility gene for SLE. In this study, we performed a quantitative reverse transcription polymerase chain reaction (RT-PCR) in 62 patients with SLE and 69 controls to investigate the different expression levels of IL-28RA in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy controls and the association between IL28RA expression and systemic lupus erythematosus disease activity index (SLEDAI) or the variant of the singlenucleotide polymorphism (SNP) rs4649203. The expression levels of IL-28RA messenger RNA (mRNA) in SLE patients were significantly increased compared with those of healthy controls. In addition, there were also significant differences in the expression levels of IL-28RA between active (SLEDAI≥6) or inactive (SLEDAI0.05), separately. Genotypes were compared using the independent-samples t test. Spearman’s correlation coefficient
Clin Rheumatol
rank test was used to assess the association between IL-28RA mRNA expression levels and the SLEDAI scores. Analysis was performed with Statistical Package for the Social Science (SPSS) version 20.0. (SPSS Inc., Chicago, IL, USA). Data were expressed as mean±standard deviation. A P value of
