Clin Chem Lab Med 2014; 52(9): 1367–1377
Jia-Sin Yang, Mu-Kuan Chen, Shun-Fa Yang, Yu-Chao Chang, Shih-Chi Su, Hui-Ling Chiou, Ming-Hsien Chien* and Chiao-Wen Lin*
Increased expression of carbonic anhydrase IX in oral submucous fibrosis and oral squamous cell carcinoma Abstract Background: Cumulative evidence has demonstrated that carbonic anhydrase IX (CAIX) is upregulated in many types of human cancers. We attempted to evaluate plasma levels of CAIX in patients with oral cancer and investigated whether plasma CAIX is correlated with the progression of this disease. Method: In total, 191 patients with oral cancer, 30 patients with oral submucous fibrosis and 100 controls were recruited in this study. The plasma samples were collected and the levels of soluble CAIX in plasma were determined by the enzyme-linked immunosorbent assay (ELISA). Furthermore, the normal buccal mucosa fibroblast was challenged by arecoline, the major areca nut alkaloid, to assess the relationship between the levels of CAIX and areca nut chewing in oral cancer patients. Results: Results showed that patients with oral cancer exhibited significantly higher levels of soluble CAIX *Corresponding authors: Ming-Hsien Chien and Dr. Chiao-Wen Lin, Institute of Oral Sciences, Chung Shan Medical University, 110 Chien-Kuo N. Road, Section 1, Taichung 402, Taiwan, Phone: +886 4 24739595 ext. 34253, Fax: +886 4 24723229, E-mail: [email protected], [email protected] Jia-Sin Yang and Shun-Fa Yang: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan Mu-Kuan Chen: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; and Department of Otorhinolaryngology-Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan Yu-Chao Chang: School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; and Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan Shih-Chi Su: Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA Hui-Ling Chiou: School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan Ming-Hsien Chien: Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; and Wan Fan Hospital, Taipei Medical University, Taipei, Taiwan Chiao-Wen Lin: Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan; and Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan
compared to controls (p
Jia-Sin Yang, Mu-Kuan Chen, Shun-Fa Yang, Yu-Chao Chang, Shih-Chi Su, Hui-Ling Chiou, Ming-Hsien Chien* and Chiao-Wen Lin*
Increased expression of carbonic anhydrase IX in oral submucous fibrosis and oral squamous cell carcinoma Abstract Background: Cumulative evidence has demonstrated that carbonic anhydrase IX (CAIX) is upregulated in many types of human cancers. We attempted to evaluate plasma levels of CAIX in patients with oral cancer and investigated whether plasma CAIX is correlated with the progression of this disease. Method: In total, 191 patients with oral cancer, 30 patients with oral submucous fibrosis and 100 controls were recruited in this study. The plasma samples were collected and the levels of soluble CAIX in plasma were determined by the enzyme-linked immunosorbent assay (ELISA). Furthermore, the normal buccal mucosa fibroblast was challenged by arecoline, the major areca nut alkaloid, to assess the relationship between the levels of CAIX and areca nut chewing in oral cancer patients. Results: Results showed that patients with oral cancer exhibited significantly higher levels of soluble CAIX *Corresponding authors: Ming-Hsien Chien and Dr. Chiao-Wen Lin, Institute of Oral Sciences, Chung Shan Medical University, 110 Chien-Kuo N. Road, Section 1, Taichung 402, Taiwan, Phone: +886 4 24739595 ext. 34253, Fax: +886 4 24723229, E-mail: [email protected], [email protected] Jia-Sin Yang and Shun-Fa Yang: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan Mu-Kuan Chen: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; and Department of Otorhinolaryngology-Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan Yu-Chao Chang: School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; and Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan Shih-Chi Su: Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA Hui-Ling Chiou: School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan Ming-Hsien Chien: Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; and Wan Fan Hospital, Taipei Medical University, Taipei, Taiwan Chiao-Wen Lin: Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan; and Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan
compared to controls (p
