Appl Biochem Biotechnol DOI 10.1007/s12010-014-1353-9
Improving Enzymatic Hydrolysis of Corn Stover Pretreated by Ethylene Glycol-Perchloric Acid-Water Mixture Yu-Cai He & Feng Liu & Lei Gong & Ting Lu & Yun Ding & Dan-Ping Zhang & Qing Qing & Yue Zhang
Received: 30 August 2014 / Accepted: 30 October 2014 # Springer Science+Business Media New York 2014
Abstract To improve the enzymatic saccharification of lignocellulosic biomass, a mixture of ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) was used for pretreating corn stover in this study. After the optimization in oil-bath system, the optimum pretreatment temperature and time were 130 °C and 30 min, respectively. After the saccharification of 10 g/L pretreated corn stover for 48 h, the saccharification rate was obtained in the yield of 77.4 %. To decrease pretreatment temperature and shorten pretreatment time, ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) media under microwave irradiation was employed to pretreat corn stover effectively at 100 °C and 200 W for 5 min. Finally, the recovered hydrolyzates containing glucose obtained from the enzymatic hydrolysis of pretreated corn stovers could be fermented into ethanol efficiently. These results would be helpful for developing a cost-effective pretreatment combined with enzymatic saccharification of cellulosic materials for the production of lignocellulosic ethanol. Keyword Corn stover . Ethylene glycol . Perchloric acid . Pretreatment . Saccharification
Introduction Lignocellulosic biomass has received considerable attention since it is the most abundant resource in nature that can be biologically converted to biofuels and other value-added biobased chemicals [1–7]. To make these cellulosic materials more susceptible for hydrolysis by cellulases, an effective pretreatment is essential to soften its tough assembled structure of Y.
Improving Enzymatic Hydrolysis of Corn Stover Pretreated by Ethylene Glycol-Perchloric Acid-Water Mixture Yu-Cai He & Feng Liu & Lei Gong & Ting Lu & Yun Ding & Dan-Ping Zhang & Qing Qing & Yue Zhang
Received: 30 August 2014 / Accepted: 30 October 2014 # Springer Science+Business Media New York 2014
Abstract To improve the enzymatic saccharification of lignocellulosic biomass, a mixture of ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) was used for pretreating corn stover in this study. After the optimization in oil-bath system, the optimum pretreatment temperature and time were 130 °C and 30 min, respectively. After the saccharification of 10 g/L pretreated corn stover for 48 h, the saccharification rate was obtained in the yield of 77.4 %. To decrease pretreatment temperature and shorten pretreatment time, ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) media under microwave irradiation was employed to pretreat corn stover effectively at 100 °C and 200 W for 5 min. Finally, the recovered hydrolyzates containing glucose obtained from the enzymatic hydrolysis of pretreated corn stovers could be fermented into ethanol efficiently. These results would be helpful for developing a cost-effective pretreatment combined with enzymatic saccharification of cellulosic materials for the production of lignocellulosic ethanol. Keyword Corn stover . Ethylene glycol . Perchloric acid . Pretreatment . Saccharification
Introduction Lignocellulosic biomass has received considerable attention since it is the most abundant resource in nature that can be biologically converted to biofuels and other value-added biobased chemicals [1–7]. To make these cellulosic materials more susceptible for hydrolysis by cellulases, an effective pretreatment is essential to soften its tough assembled structure of Y.
