www.nature.com/scientificreports
OPEN
received: 30 September 2016 accepted: 01 March 2017 Published: 03 April 2017
Impact of Cell Composition and Geometry on Human Induced Pluripotent Stem Cells-Derived Engineered Cardiac Tissue Takeichiro Nakane1,2,3, Hidetoshi Masumoto1,2,3, Joseph P. Tinney1,4, Fangping Yuan1,4, William J. Kowalski1,4, Fei Ye1,4, Amanda J. LeBlanc5, Ryuzo Sakata3, Jun K. Yamashita2 & Bradley B. Keller1,4 The current study describes a scalable, porous large-format engineered cardiac tissue (LF-ECT) composed of human induced pluripotent stem cells (hiPSCs) derived multiple lineage cardiac cells with varied 3D geometries and cell densities developed towards the goal of scale-up for large animal preclinical studies. We explored multiple 15 × 15 mm ECT geometries using molds with rectangular internal staggered posts (mesh, ME), without posts (plain sheet, PS), or long parallel posts (multiple linear bundles, ML) and a gel matrix containing hiPSC-derived cardiomyocytes, endothelial, and vascular mural cells matured in vitro for 14 days. ME-ECTs displayed the lowest dead cell ratio (p
OPEN
received: 30 September 2016 accepted: 01 March 2017 Published: 03 April 2017
Impact of Cell Composition and Geometry on Human Induced Pluripotent Stem Cells-Derived Engineered Cardiac Tissue Takeichiro Nakane1,2,3, Hidetoshi Masumoto1,2,3, Joseph P. Tinney1,4, Fangping Yuan1,4, William J. Kowalski1,4, Fei Ye1,4, Amanda J. LeBlanc5, Ryuzo Sakata3, Jun K. Yamashita2 & Bradley B. Keller1,4 The current study describes a scalable, porous large-format engineered cardiac tissue (LF-ECT) composed of human induced pluripotent stem cells (hiPSCs) derived multiple lineage cardiac cells with varied 3D geometries and cell densities developed towards the goal of scale-up for large animal preclinical studies. We explored multiple 15 × 15 mm ECT geometries using molds with rectangular internal staggered posts (mesh, ME), without posts (plain sheet, PS), or long parallel posts (multiple linear bundles, ML) and a gel matrix containing hiPSC-derived cardiomyocytes, endothelial, and vascular mural cells matured in vitro for 14 days. ME-ECTs displayed the lowest dead cell ratio (p
