Original Paper ORL 1992;54:85-90
a Department of Otolaryngology, Niigata University School of Medicine, b Research Laboratory for Molecular Genetics, Niigata University, Niigata, Japan
Key Words Olfactory epithelium Immunohistochemistry Fourth cell type Human Guinea pig Spot-35 protein S-100 protein
Immunohistochemical Study of the Fourth Cell Type in the Olfactory Epithelium in Guinea Pigs and in a Patient
Abstract The mammalian olfactory epithelium consists of supporting cells, olfactory receptor cells, basal cells and a fourth cell type, which has recently been dis covered. In this study we examine this fourth cell type using immunohisto chemical techniques. Anti-Purkinje-specific spot-35 protein (S-35), anti-S-100 protein (S-100), anti-neuron-specific enolase (NSE), antichromogranin A and antisynaptophysin antisera were used for the immunostaining. The fourth cell type immunoreacted only to anti-S-35 antiserum and did not react to other antisera in guinea pigs. On the other hand, in the human S-100 immunoreactivity was seen in the fourth cell type as well as weak reactivity to S-35. NSE immunoreactivity was found only in the olfactory receptor cells of guinea pigs and the human. From these results it is assumed that this fourth cell type is a second chemoreceptor different from the olfactory receptor cells because S-35 and S-100 are Ca++-binding proteins.
Introduction
Materials and Methods
The olfactory epithelium of mammals is fundamen tally considered to consist of three types of cells: support ing cells, olfactory receptor cells and basal cells. Recently a fourth cell type was observed and its morphology and function have been examined [1,2]. Previously, we have reported briefly on the immunohistochemical Findings of the fourth cell type in guinea pigs [3]. In this study we examine these in more detail in guinea pigs and also in human olfactory epithelium.
Received. May 28. 1991 Accepted after revision: August 27, 1991
Materials Four adult male guinea pigs (body weight about 400 g) were used. The animals were anesthetized with sodium pentobarbital (Nembu tal® , 150-200 mg/kg intraperitoneally) and perfused through the heart with 150 ml saline followed by 200 ml Bouin’s fluid. The olfac tory mucosa was obtained from the superior posterior portion of the nasal septum. Human material was obtained from a patient (52-year-old fe male) with her consent who had complained of anosmia after intra cranial surgery for an anterior commissure aneurysm, using a special biopsy instrument (Nakano’s forceps type 3) which is in general use in our clinic.
Masuo Yamagishi. MD Department of Otolary ngology Niigata University School of Medicine Asahimachi 1. Niigata. 951 (Japan)
© 1992 S. Karger AG. Basel 0301-1569/92/0542-0085 $2.75/0
Downloaded by: Univ. of California Santa Barbara 128.111.121.42 - 3/3/2018 12:13:42 AM
Masuo Yamagishi* Hideo Nakamuraa Yuichi Nakanoa Ryozoh Kuwanob
a Department of Otolaryngology, Niigata University School of Medicine, b Research Laboratory for Molecular Genetics, Niigata University, Niigata, Japan
Key Words Olfactory epithelium Immunohistochemistry Fourth cell type Human Guinea pig Spot-35 protein S-100 protein
Immunohistochemical Study of the Fourth Cell Type in the Olfactory Epithelium in Guinea Pigs and in a Patient
Abstract The mammalian olfactory epithelium consists of supporting cells, olfactory receptor cells, basal cells and a fourth cell type, which has recently been dis covered. In this study we examine this fourth cell type using immunohisto chemical techniques. Anti-Purkinje-specific spot-35 protein (S-35), anti-S-100 protein (S-100), anti-neuron-specific enolase (NSE), antichromogranin A and antisynaptophysin antisera were used for the immunostaining. The fourth cell type immunoreacted only to anti-S-35 antiserum and did not react to other antisera in guinea pigs. On the other hand, in the human S-100 immunoreactivity was seen in the fourth cell type as well as weak reactivity to S-35. NSE immunoreactivity was found only in the olfactory receptor cells of guinea pigs and the human. From these results it is assumed that this fourth cell type is a second chemoreceptor different from the olfactory receptor cells because S-35 and S-100 are Ca++-binding proteins.
Introduction
Materials and Methods
The olfactory epithelium of mammals is fundamen tally considered to consist of three types of cells: support ing cells, olfactory receptor cells and basal cells. Recently a fourth cell type was observed and its morphology and function have been examined [1,2]. Previously, we have reported briefly on the immunohistochemical Findings of the fourth cell type in guinea pigs [3]. In this study we examine these in more detail in guinea pigs and also in human olfactory epithelium.
Received. May 28. 1991 Accepted after revision: August 27, 1991
Materials Four adult male guinea pigs (body weight about 400 g) were used. The animals were anesthetized with sodium pentobarbital (Nembu tal® , 150-200 mg/kg intraperitoneally) and perfused through the heart with 150 ml saline followed by 200 ml Bouin’s fluid. The olfac tory mucosa was obtained from the superior posterior portion of the nasal septum. Human material was obtained from a patient (52-year-old fe male) with her consent who had complained of anosmia after intra cranial surgery for an anterior commissure aneurysm, using a special biopsy instrument (Nakano’s forceps type 3) which is in general use in our clinic.
Masuo Yamagishi. MD Department of Otolary ngology Niigata University School of Medicine Asahimachi 1. Niigata. 951 (Japan)
© 1992 S. Karger AG. Basel 0301-1569/92/0542-0085 $2.75/0
Downloaded by: Univ. of California Santa Barbara 128.111.121.42 - 3/3/2018 12:13:42 AM
Masuo Yamagishi* Hideo Nakamuraa Yuichi Nakanoa Ryozoh Kuwanob
