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ChemComm Accepted Manuscript
This article can be cited before page numbers have been issued, to do this please use: S. H.C. Askes, N. L. Mora, R. Harkes, R. I. Koning, A. J. Koster, T. Schmidt, A. kros and S. Bonnet, Chem. Commun., 2015, DOI: 10.1039/C5CC02197A.
This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication. Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available. You can find more information about Accepted Manuscripts in the Information for Authors. Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content. The journal’s standard Terms & Conditions and the Ethical guidelines still apply. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.
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ChemComm
Journal Name
ChemComm Accepted Manuscript
RSCPublishing View Article Online
DOI: 10.1039/C5CC02197A
COMMUNICATION
Imaging the lipid bilayer of giant unilamellar vesicles using Cite this: DOI: 10.1039/x0xx00000x
Sven H.C. Askes,a,‡ Néstor López Mora,a,‡ Rolf Harkes,b Roman I. Koning,c Bram Koster,c Thomas Schmidt,b Alexander Kros,a Sylvestre Bonnet.a,*
DOI: 10.1039/x0xx00000x www.rsc.org/
Red-to-blue triplet-triplet annihilation upconversion was obtained in giant unilamellar vesicles. The upconverted light was homogeneously distributed across the membrane and could be utilized for the imaging of individual giant vesicles in three dimensions. These results show the great potential of TTA-UC for imaging applications under anoxic conditions. Upconversion luminescence (bio)imaging offers great advantages over conventional imaging. The absence of autofluorescence results in high contrast images, while photons of low energy, i.e. within the phototherapeutic window (600-1000 nm), afford higher tissue penetration and negligible irradiation damage. For these reasons lanthanoid-based upconverting nanoparticles (UCNPs), for example, have attracted much interest.1, 2 However, UCNPs suffer from several disadvantages, such as the need for high excitation power, the low absorption cross section of lanthanoid ions, and low upconversion efficiency in aqueous solution (typically ≤0.5%).2 In contrast, triplet-triplet annihilation upconversion (TTA-UC) requires low excitation power (
ChemComm Accepted Manuscript
This article can be cited before page numbers have been issued, to do this please use: S. H.C. Askes, N. L. Mora, R. Harkes, R. I. Koning, A. J. Koster, T. Schmidt, A. kros and S. Bonnet, Chem. Commun., 2015, DOI: 10.1039/C5CC02197A.
This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication. Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available. You can find more information about Accepted Manuscripts in the Information for Authors. Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content. The journal’s standard Terms & Conditions and the Ethical guidelines still apply. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.
www.rsc.org/chemcomm
Page 1 of 4
ChemComm
Journal Name
ChemComm Accepted Manuscript
RSCPublishing View Article Online
DOI: 10.1039/C5CC02197A
COMMUNICATION
Imaging the lipid bilayer of giant unilamellar vesicles using Cite this: DOI: 10.1039/x0xx00000x
Sven H.C. Askes,a,‡ Néstor López Mora,a,‡ Rolf Harkes,b Roman I. Koning,c Bram Koster,c Thomas Schmidt,b Alexander Kros,a Sylvestre Bonnet.a,*
DOI: 10.1039/x0xx00000x www.rsc.org/
Red-to-blue triplet-triplet annihilation upconversion was obtained in giant unilamellar vesicles. The upconverted light was homogeneously distributed across the membrane and could be utilized for the imaging of individual giant vesicles in three dimensions. These results show the great potential of TTA-UC for imaging applications under anoxic conditions. Upconversion luminescence (bio)imaging offers great advantages over conventional imaging. The absence of autofluorescence results in high contrast images, while photons of low energy, i.e. within the phototherapeutic window (600-1000 nm), afford higher tissue penetration and negligible irradiation damage. For these reasons lanthanoid-based upconverting nanoparticles (UCNPs), for example, have attracted much interest.1, 2 However, UCNPs suffer from several disadvantages, such as the need for high excitation power, the low absorption cross section of lanthanoid ions, and low upconversion efficiency in aqueous solution (typically ≤0.5%).2 In contrast, triplet-triplet annihilation upconversion (TTA-UC) requires low excitation power (
