Proc. Nat!. Acad. Sci. USA Vol. 87, pp. 4514-4518, June 1990 Biochemistry

Imaging of DNA sequences with chemiluminescence (Maxam-Gilbert DNA sequencing/multiplex sequencing/enzyme substrates/alkaline phosphatase/biotinylated oligonucleotide probes)

RICHARD TIZARD*, RICHARD L. CATE*, K. L. RAMACHANDRAN*, MARK WYSK*, JOHN C. VOYTAt, OWEN J. MURPHYt, AND IRENA BRONSTEINtt *Biogen, Inc., 14 Cambridge Center,

Cambridge, MA 02142; and tTropix, Inc., 47 Wiggins Avenue, Bedford, MA 01730

Communicated by George S. Hammond, April 9, 1990

We have coupled a chemiluminescent detecABSTRACT tion method that uses an alkaline phosphatase label to the genomic DNA sequencing protocol of Church and Gilbert [Church, G. M. & Gilbert, W. (1984) Proc. Nadl. Acad. Sci. USA 81, 1991-1995]. Images of sequence ladders are obtained on x-ray film with exposure times of

Imaging of DNA sequences with chemiluminescence.

We have coupled a chemiluminescent detection method that uses an alkaline phosphatase label to the genomic DNA sequencing protocol of Church and Gilbe...
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