RESEARCH ARTICLE
Identification of Structural Features of Condensed Tannins That Affect Protein Aggregation Honorata M. Ropiak1*, Peter Lachmann2, Aina Ramsay1, Rebecca J. Green3, Irene Mueller-Harvey1*
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1 School of Agriculture, Policy and Development; Chemistry and Biochemistry Laboratory, University of Reading, Reading, Berkshire, United Kingdom, 2 Independent Researcher, Bookham, Surrey, United Kingdom, 3 School of Chemistry, Food and Pharmacy; University of Reading, Reading, Berkshire, United Kingdom * [email protected] (HMR); [email protected] (IMH)
Abstract
Published: January 26, 2017
A diverse panel of condensed tannins was used to resolve the confounding effects of size and subunit composition seen previously in tannin-protein interactions. Turbidimetry revealed that size in terms of mean degree of polymerisation (mDP) or average molecular weight (amw) was the most important tannin parameter. The smallest tannin with the relatively largest effect on protein aggregation had an mDP of ~7. The average size was significantly correlated with aggregation of bovine serum albumin, BSA (mDP: r = -0.916; amw: r = -0.925; p
Identification of Structural Features of Condensed Tannins That Affect Protein Aggregation Honorata M. Ropiak1*, Peter Lachmann2, Aina Ramsay1, Rebecca J. Green3, Irene Mueller-Harvey1*
a1111111111 a1111111111 a1111111111 a1111111111 a1111111111
1 School of Agriculture, Policy and Development; Chemistry and Biochemistry Laboratory, University of Reading, Reading, Berkshire, United Kingdom, 2 Independent Researcher, Bookham, Surrey, United Kingdom, 3 School of Chemistry, Food and Pharmacy; University of Reading, Reading, Berkshire, United Kingdom * [email protected] (HMR); [email protected] (IMH)
Abstract
Published: January 26, 2017
A diverse panel of condensed tannins was used to resolve the confounding effects of size and subunit composition seen previously in tannin-protein interactions. Turbidimetry revealed that size in terms of mean degree of polymerisation (mDP) or average molecular weight (amw) was the most important tannin parameter. The smallest tannin with the relatively largest effect on protein aggregation had an mDP of ~7. The average size was significantly correlated with aggregation of bovine serum albumin, BSA (mDP: r = -0.916; amw: r = -0.925; p
