Accepted Manuscript Title: Hypertension in women with polycystic ovary syndrome: prevalence and associated cardiovascular risk factors Author: Yuhua Shi Yuqian Cui Xuedong Sun Gang Ma Zengxiang Ma Qin Gao Zi-jiang Chen PII: DOI: Reference:
S0301-2115(13)00584-8 http://dx.doi.org/doi:10.1016/j.ejogrb.2013.11.011 EURO 8328
To appear in:
EURO
Received date: Revised date: Accepted date:
18-6-2013 9-9-2013 18-11-2013
Please cite this article as: Shi Y, Cui Y, Sun X, Ma G, Ma Z, Gao Q, Chen Z-j, Hypertension in women with polycystic ovary syndrome: prevalence and associated cardiovascular risk factors, European Journal of Obstetrics & Gynecology and Reproductive Biology (2013), http://dx.doi.org/10.1016/j.ejogrb.2013.11.011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Condensation (one sentence summary)
Condensation The likelihood of long-term complications is higher in hypertensive PCOS patients
Ac
ce pt
ed
M
an
us
cr
ip t
compared with normotensive PCOS patients.
Page 1 of 20
*Manuscript
Hypertension in women with polycystic ovary syndrome: prevalence and associated cardiovascular risk factors
ip t
Yuhua Shia, Yuqian Cuib, Xuedong Sunc, Gang Maa, Zengxiang Maa, Qin Gaoa,
Reproductive Medicine Hospital Affiliated to Shandong University, Jinan, China
b
Renji Hospital, Shanghai Jiao tong University School of Medicine
Neurology Department, Provincial Hospital Affiliated to Shandong University
ce pt
ed
M
an
c
us
a
cr
Zi-jiang Chena,*
Ac
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65
*
Corresponding author. Address: Reproductive Medicine Hospital Affiliated to Shandong University,
157 Jingliu Road, Jinan, 250021, China. Tel.: +86 531 85187856; fax: +86 531 87068226. E-mail address: [email protected] (Z-J. Chen).
Page 2 of 20
ABSTRACT Objective: To determine the prevalence of hypertension in women with polycystic ovary syndrome (PCOS). Study design: Women with PCOS (n=3396) and controls (n=1891) from a Han
ip t
Chinese population were analysed retrospectively in terms of ovarian appearance on ultrasound, glucose tolerance test, hormones, lipoproteins, anthropomorphic
cr
measurements and blood pressure.
us
Results: The prevalence of hypertension was 19.2% in the women with PCOS, which was much higher than that in the controls (11.9%). After matching for body mass
an
index, the hypertensive PCOS group had higher glucose, insulin, HOMA-IR and lipid levels than the normotensive PCOS group; these differences were significant (p
M
60 ng/dl [2,3], excluding existence of other hyperandrogenic diseases (Fig. 1). The women with PCOS were not receiving any medication (including oral contraceptive pills for at least 3 months). Study subjects were assessed
Page 4 of 20
at the Clinical Research Centre after a 12-h fast. A 240-min oral glucose tolerance test was performed with blood samples drawn for testing insulin and glucose levels at 0 and 120 min. Blood samples were drawn for testing all other parameters (i.e. lipid and hormone levels) at baseline (on day 2–3 of a spontaneous menstrual cycle or
ip t
progestin-induced menstrual bleeding with oligomenorrhoea/amenorrhoea) before ingestion of the glucose load. Waist and hip circumferences were measured in
cr
centimetres at the level of the umbilicus and the greater trochanters, respectively, by
us
the same examiner. Mean systolic and diastolic blood pressure were determined from two blood pressure readings taken at 30-min intervals with the subject seated quietly.
an
Blood pressure ≥140/90 mmHg was defined as hypertension.
M
total
testosterone
and
ed
Serum follicle-stimulating hormone, luteinizing hormone, oestradiol, prolactin, thyroid-stimulating
hormone
were
measured
by
ce pt
chemiluminescence immunization (Beckman Access Health Company, Chaska, MN, USA). Plasma glucose was measured using the oxidase method (AU640 automatic biochemistry
analyzer;
Olympus
Company,
Hamburg,
Germany)
with
a
between-batch coefficient of variation of
S0301-2115(13)00584-8 http://dx.doi.org/doi:10.1016/j.ejogrb.2013.11.011 EURO 8328
To appear in:
EURO
Received date: Revised date: Accepted date:
18-6-2013 9-9-2013 18-11-2013
Please cite this article as: Shi Y, Cui Y, Sun X, Ma G, Ma Z, Gao Q, Chen Z-j, Hypertension in women with polycystic ovary syndrome: prevalence and associated cardiovascular risk factors, European Journal of Obstetrics & Gynecology and Reproductive Biology (2013), http://dx.doi.org/10.1016/j.ejogrb.2013.11.011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Condensation (one sentence summary)
Condensation The likelihood of long-term complications is higher in hypertensive PCOS patients
Ac
ce pt
ed
M
an
us
cr
ip t
compared with normotensive PCOS patients.
Page 1 of 20
*Manuscript
Hypertension in women with polycystic ovary syndrome: prevalence and associated cardiovascular risk factors
ip t
Yuhua Shia, Yuqian Cuib, Xuedong Sunc, Gang Maa, Zengxiang Maa, Qin Gaoa,
Reproductive Medicine Hospital Affiliated to Shandong University, Jinan, China
b
Renji Hospital, Shanghai Jiao tong University School of Medicine
Neurology Department, Provincial Hospital Affiliated to Shandong University
ce pt
ed
M
an
c
us
a
cr
Zi-jiang Chena,*
Ac
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65
*
Corresponding author. Address: Reproductive Medicine Hospital Affiliated to Shandong University,
157 Jingliu Road, Jinan, 250021, China. Tel.: +86 531 85187856; fax: +86 531 87068226. E-mail address: [email protected] (Z-J. Chen).
Page 2 of 20
ABSTRACT Objective: To determine the prevalence of hypertension in women with polycystic ovary syndrome (PCOS). Study design: Women with PCOS (n=3396) and controls (n=1891) from a Han
ip t
Chinese population were analysed retrospectively in terms of ovarian appearance on ultrasound, glucose tolerance test, hormones, lipoproteins, anthropomorphic
cr
measurements and blood pressure.
us
Results: The prevalence of hypertension was 19.2% in the women with PCOS, which was much higher than that in the controls (11.9%). After matching for body mass
an
index, the hypertensive PCOS group had higher glucose, insulin, HOMA-IR and lipid levels than the normotensive PCOS group; these differences were significant (p
M
60 ng/dl [2,3], excluding existence of other hyperandrogenic diseases (Fig. 1). The women with PCOS were not receiving any medication (including oral contraceptive pills for at least 3 months). Study subjects were assessed
Page 4 of 20
at the Clinical Research Centre after a 12-h fast. A 240-min oral glucose tolerance test was performed with blood samples drawn for testing insulin and glucose levels at 0 and 120 min. Blood samples were drawn for testing all other parameters (i.e. lipid and hormone levels) at baseline (on day 2–3 of a spontaneous menstrual cycle or
ip t
progestin-induced menstrual bleeding with oligomenorrhoea/amenorrhoea) before ingestion of the glucose load. Waist and hip circumferences were measured in
cr
centimetres at the level of the umbilicus and the greater trochanters, respectively, by
us
the same examiner. Mean systolic and diastolic blood pressure were determined from two blood pressure readings taken at 30-min intervals with the subject seated quietly.
an
Blood pressure ≥140/90 mmHg was defined as hypertension.
M
total
testosterone
and
ed
Serum follicle-stimulating hormone, luteinizing hormone, oestradiol, prolactin, thyroid-stimulating
hormone
were
measured
by
ce pt
chemiluminescence immunization (Beckman Access Health Company, Chaska, MN, USA). Plasma glucose was measured using the oxidase method (AU640 automatic biochemistry
analyzer;
Olympus
Company,
Hamburg,
Germany)
with
a
between-batch coefficient of variation of
