Accepted Article

Received Date : 14-Feb-2014 Revised Date : 19-May-2014 Accepted Date : 19-May-2014 Article type

: Original Article

Hyaluronic acid production with Corynebacterium glutamicum: effect of media composition on yield and molecular weight

Running Title: Hyaluronic acid production with C. glutamicum

Jana Hoffmann and Josef Altenbuchner* Institute of Industrial Genetics, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany

*Corresponding author Phone: +49 711 685 675 91, Fax: +49 711 685 669 73 E-mail: [email protected]

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as an 'Accepted Article', doi: 10.1111/jam.12553 This article is protected by copyright. All rights reserved.

Accepted Article

Abstract Aim: Corynebacterium glutamicum was tested as an alternative host for heterologous production of hyaluronic acid (HA). Methods and Results: A set of expression vectors containing hasA, encoding HA synthase from Streptococcus equi subsp. zooepidermicus, alone or in combination with genes encoding enzymes for HA precursor production (hasB, hasC, glmU from Pseudomonas putida KT2440) or bacterial haemoglobin (vgb from Vitreoscilla sp.) was constructed. Recombinant C. glutamicum strains were cultivated in two different minimal media, CGXII and MEK700. Hyaluronic acid was isolated from the culture broth by ethanol precipitation or ultrafiltration. Analyses of the isolated HA revealed that overall production was higher in CGXII medium (1,241 mg l-1) than in MEK700 medium (363 mg l-1) but molecular weight of the product was higher in MEK700 (>1.4 MDa) than in CGXII (1.4 MDa) or CGXII (

Hyaluronic acid production with Corynebacterium glutamicum: effect of media composition on yield and molecular weight.

Corynebacterium glutamicum was tested as an alternative host for heterologous production of hyaluronic acid (HA)...
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