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Cell Cycle Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/kccy20
Hit-and-run lymphomagenesis by the Bcl6 oncogene a
bc
a
bc
Michael R Green , Carolina Vicente-Dueñas , Ash A. Alizadeh & Isidro Sanchez-García a
Divisions of Oncology and of Hematology; School of Medicine; Stanford University; Stanford, CA USA b
Experimental Therapeutics and Translational Oncology Program; Instituto de Biología Molecular y Celular del Cáncer; CSIC/Universidad de Salamanca; Salamanca, Spain c
Institute of Biomedical Research of Salamanca (IBSAL); Salamanca, Spain Published online: 27 May 2014.
Click for updates To cite this article: Michael R Green, Carolina Vicente-Dueñas, Ash A. Alizadeh & Isidro Sanchez-García (2014) Hit-and-run lymphomagenesis by the Bcl6 oncogene, Cell Cycle, 13:12, 1831-1832, DOI: 10.4161/cc.29326 To link to this article: http://dx.doi.org/10.4161/cc.29326
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Versions of published Taylor & Francis and Routledge Open articles and Taylor & Francis and Routledge Open Select articles posted to institutional or subject repositories or any other third-party website are without warranty from Taylor & Francis of any kind, either expressed or implied, including, but not limited to, warranties of merchantability, fitness for a particular purpose, or non-infringement. Any opinions and views expressed in this article are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor & Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Terms & Conditions of access and use can be found at http://www.tandfonline.com/page/terms-and-conditions It is essential that you check the license status of any given Open and Open Select article to confirm conditions of access and use.
Editorials: Cell Cycle Features
Editorials: Cell Cycle Features
Cell Cycle 13:12, 1831–1832; June 15, 2014; © 2014 Landes Bioscience
Hit-and-run lymphomagenesis by the Bcl6 oncogene Michael R Green1,†, Carolina Vicente-Dueñas2,3,†, Ash A Alizadeh1,*, and Isidro Sánchez-García2,3,* Divisions of Oncology and of Hematology; School of Medicine; Stanford University; Stanford, CA USA; 2Experimental Therapeutics and Translational Oncology Program; Instituto de Biología Molecular y Celular del Cáncer; CSIC/Universidad de Salamanca; Salamanca, Spain; 3Institute of Biomedical Research of Salamanca (IBSAL); Salamanca, Spain
1
These authors contributed equally to this work.
Downloaded by [198.91.36.79] at 03:31 22 February 2015
†
Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoid malignancy in humans, accounting for nearly 40% of all non-Hodgkin lymphomas.1 In spite of recent advances in monoclonal antibody therapy, up to 40% of patients eventually relapse and die of their disease after treatment with chemo-immunotherapy regimens. Given the inability to cure many patients with DLBCL, and the significant toxicity of current therapies, better treatment strategies are needed. Functional genomics studies have allowed DLBCLs to be subdivided into biologically and clinically relevant disease subtypes, which are called germinal center B-cell (GCB-like) type or activated B-cell (ABC-like) type, based on their transcriptional similarity to the respective populations of primary B cells.2 The ABC-like DLBCLs are generally more refractory to treatment than the GCB-like subtype. In spite of these advances, much remains unknown regarding the genetic basis and pathophysiology of this aggressive and highly heterogeneous B-cell malignancy. In this regard, we have recently identified gain of 3q27.2 as being significantly associated with adverse outcome in DLBCL and linked with the ABC-like subtype.3 This lesion includes the BCL6 oncogene, but, surprisingly, using integrative analysis of paired DNA copy number and gene expression microarray data, we found no significant overexpression of BCL6 transcript in tumors with 3q27 gain, nor did we observe coordinate repression of previously identified BCL6 target genes in these tumors.3 Furthermore,
transgenic mice that express Bcl6 specifically in mature B cells develop lymphomas in only a subset of mice,4 and these tumors align most significantly with the GCB-like stage of murine B-cell development—a very different phenotype than aggressive human ABC-like DLBCL, in which genetic alterations of BCL6 are most frequently observed. This observation led us to hypothesize that BCL6 may function as a “hit-and-run” oncogene that acts at an early stage of B-cell development to reprogram hematopoietic stem/progenitor cells (HS/PCs) for malignancy. To test this hypothesis, we generated strains of mice with restricted expression of this oncogene within HS/PCs.3 Despite lack of Bcl6 protein expression, mature non-malignant B cells from these mice show coordinate repression of Bcl6 target genes, suggesting a lasting imprint from Bcl6-mediated reprogramming within the stem/progenitor compartment. These mice go on to develop aggressive and clonal B-cell lymphomas that accumulate at a post-germinal center stage of differentiation, in line with human lymphomas in which BCL6 alterations are most frequently observed.3 These results therefore confirm that activity of the Bcl6 oncogene restricted to HS/ PCs can induce malignancies in mice that are of a post-germinal center stage of differentiation. Bcl6 mediates suppression of target genes via recruitment of factors that epigenetically modify chromatin. We hypothesized that this may elicit changes in DNA methylation, a mark that is capable acting in gene silencing memory,
and therefore investigated whether this may be a potential mechanism for Bcl6medited “hit-and-run” oncogenesis. Using genome-wide DNA methylation profiling of populations of HS/PCs and mature B-cells from wild type and Sca1Bcl6 mice, we identified broad epigenetic changes associated with the expression of Bcl6 in HS/PCs. Importantly, a significant subset of these changes were found to be maintained from HS/PCs to mature B cells in Sca1–Bcl6 mice, resulting in these populations being epigenetically more similar to each other than to their comparative population from wild-type mice.3 Together these results suggest that the “hit-and-run” role of Bcl6 activity may be manifested via epigenetic alterations. This epigenetic memory would maintain gene expression states through cell generations without a change in DNA sequence and in the absence of initiating signals. In conclusion, we have provided the first evidence of a “hit-and-run” role for Bcl6 in the oncogenesis of lymphoma. The implication of these findings is that, if ABC-like DLBCL occurs via a reprogramming-like mechanism, oncogenes that initiate tumor formation might be dispensable for tumor cell survival and/or tumor progression. In this context, mutations that activate oncogenes would have a driving role in the reprogramming process, but may act as passenger mutations thereafter, or may have a secondary role in evolved tumor cell clones.5,6 This may provide an explanation for the failure of some modern targeted therapies to clear tumor stem cells, despite being effective agents
*Correspondence to: Ash A Alizadeh; Email: [email protected]; Isidro Sánchez-García; Email: [email protected] Submitted: 05/09/2014; Accepted: 05/12/2014; Published Online: 05/27/2014 http://dx.doi.org/10.4161/cc.29326 Comment on: Green MR, et al. Nat Commun 2014; 5:3904; PMID:24887457; http://dx.doi.org/10.1038/ncomms4904
www.landesbioscience.com
Cell Cycle 1831
Downloaded by [198.91.36.79] at 03:31 22 February 2015
in vitro and in vivo.7 If BCL6 was required to maintain lymphoma-repopulating stem cells, then such a BCL6-targeted therapy might indeed prove effective in eradicating residual tumor cells when translated to the human setting. On the other hand, the fact that BCL6 can also contribute to the malignant phenotype through a hitand-run mechanism dependent on aberrant epigenetic programming of HS/PCs raises the possibility that tumor cells could emerge that become BCL6-independent. This prediction is supported by the fact that certain BCL6-positive DLBCL cell lines such as OCI-Ly8 (which contains a BCL6 translocation) and Karpas 422 are not killed by BCL6 shRNA or BCL6targeted therapy. From the therapeutic standpoint, this would indicate the need to combine BCL6 inhibitors with drugs that can kill these BCL6-independent cells. These concepts are therefore central to the full understanding of disease genesis and critical for the development of modern therapies aimed at directly addressing such genetic lesions. References 1. 2.
3.
Figure 1. Bcl6 hit-and-run oncogenesis in lymphoma. Transient introduction of Bcl6 into HS/PCs was sufficient to induce ABC-DLBCL lymphoma through specific genetic/epigenetic changes in HS/PCs that do not interfere with normal B-cell development. This model initially applies to the mouse and the role of BCL6 in human DLBCL progenitors is still unknown and an area of future investigation.
4.
5.
6.
against evolved tumor cells. For instance, BCL6 has emerged as a potentially important therapeutic target in recent years
1832
given its frequent involvement in lymphomagenesis and the fact that specific BCL6 inhibitors can kill lymphoma cells
Cell Cycle
7.
Armitage JO, et al. J Clin Oncol 1998; 16:2780-95; PMID:9704731 Alizadeh AA, et al. Nature 2000; 403:50311; PMID:10676951; http://dx.doi. org/10.1038/35000501 Green MR, et al. Nat Commun 2014; 5:3904; PMID:24887457; http://dx.doi.org/10.1038/ ncomms4904 Cattoretti G, et al. Cancer Cell 2005; 7:445-55; PMID:15894265; http://dx.doi.org/10.1016/j. ccr.2005.03.037 Vicente-Dueñas C, et al. EMBO J 2013; 32:150213; PMID:23632857; http://dx.doi.org/10.1038/ emboj.2013.97 Shin K, et al. Nat Cell Biol 2014; 16:469-78; PMID:24747439; http://dx.doi.org/10.1038/ ncb2956 Cerchietti LC, et al. Blood 2009; 113:3397-405; PMID:18927431; http://dx.doi.org/10.1182/ blood-2008-07-168773
Volume 13 Issue 12
Cell Cycle Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/kccy20
Hit-and-run lymphomagenesis by the Bcl6 oncogene a
bc
a
bc
Michael R Green , Carolina Vicente-Dueñas , Ash A. Alizadeh & Isidro Sanchez-García a
Divisions of Oncology and of Hematology; School of Medicine; Stanford University; Stanford, CA USA b
Experimental Therapeutics and Translational Oncology Program; Instituto de Biología Molecular y Celular del Cáncer; CSIC/Universidad de Salamanca; Salamanca, Spain c
Institute of Biomedical Research of Salamanca (IBSAL); Salamanca, Spain Published online: 27 May 2014.
Click for updates To cite this article: Michael R Green, Carolina Vicente-Dueñas, Ash A. Alizadeh & Isidro Sanchez-García (2014) Hit-and-run lymphomagenesis by the Bcl6 oncogene, Cell Cycle, 13:12, 1831-1832, DOI: 10.4161/cc.29326 To link to this article: http://dx.doi.org/10.4161/cc.29326
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Versions of published Taylor & Francis and Routledge Open articles and Taylor & Francis and Routledge Open Select articles posted to institutional or subject repositories or any other third-party website are without warranty from Taylor & Francis of any kind, either expressed or implied, including, but not limited to, warranties of merchantability, fitness for a particular purpose, or non-infringement. Any opinions and views expressed in this article are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor & Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Terms & Conditions of access and use can be found at http://www.tandfonline.com/page/terms-and-conditions It is essential that you check the license status of any given Open and Open Select article to confirm conditions of access and use.
Editorials: Cell Cycle Features
Editorials: Cell Cycle Features
Cell Cycle 13:12, 1831–1832; June 15, 2014; © 2014 Landes Bioscience
Hit-and-run lymphomagenesis by the Bcl6 oncogene Michael R Green1,†, Carolina Vicente-Dueñas2,3,†, Ash A Alizadeh1,*, and Isidro Sánchez-García2,3,* Divisions of Oncology and of Hematology; School of Medicine; Stanford University; Stanford, CA USA; 2Experimental Therapeutics and Translational Oncology Program; Instituto de Biología Molecular y Celular del Cáncer; CSIC/Universidad de Salamanca; Salamanca, Spain; 3Institute of Biomedical Research of Salamanca (IBSAL); Salamanca, Spain
1
These authors contributed equally to this work.
Downloaded by [198.91.36.79] at 03:31 22 February 2015
†
Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoid malignancy in humans, accounting for nearly 40% of all non-Hodgkin lymphomas.1 In spite of recent advances in monoclonal antibody therapy, up to 40% of patients eventually relapse and die of their disease after treatment with chemo-immunotherapy regimens. Given the inability to cure many patients with DLBCL, and the significant toxicity of current therapies, better treatment strategies are needed. Functional genomics studies have allowed DLBCLs to be subdivided into biologically and clinically relevant disease subtypes, which are called germinal center B-cell (GCB-like) type or activated B-cell (ABC-like) type, based on their transcriptional similarity to the respective populations of primary B cells.2 The ABC-like DLBCLs are generally more refractory to treatment than the GCB-like subtype. In spite of these advances, much remains unknown regarding the genetic basis and pathophysiology of this aggressive and highly heterogeneous B-cell malignancy. In this regard, we have recently identified gain of 3q27.2 as being significantly associated with adverse outcome in DLBCL and linked with the ABC-like subtype.3 This lesion includes the BCL6 oncogene, but, surprisingly, using integrative analysis of paired DNA copy number and gene expression microarray data, we found no significant overexpression of BCL6 transcript in tumors with 3q27 gain, nor did we observe coordinate repression of previously identified BCL6 target genes in these tumors.3 Furthermore,
transgenic mice that express Bcl6 specifically in mature B cells develop lymphomas in only a subset of mice,4 and these tumors align most significantly with the GCB-like stage of murine B-cell development—a very different phenotype than aggressive human ABC-like DLBCL, in which genetic alterations of BCL6 are most frequently observed. This observation led us to hypothesize that BCL6 may function as a “hit-and-run” oncogene that acts at an early stage of B-cell development to reprogram hematopoietic stem/progenitor cells (HS/PCs) for malignancy. To test this hypothesis, we generated strains of mice with restricted expression of this oncogene within HS/PCs.3 Despite lack of Bcl6 protein expression, mature non-malignant B cells from these mice show coordinate repression of Bcl6 target genes, suggesting a lasting imprint from Bcl6-mediated reprogramming within the stem/progenitor compartment. These mice go on to develop aggressive and clonal B-cell lymphomas that accumulate at a post-germinal center stage of differentiation, in line with human lymphomas in which BCL6 alterations are most frequently observed.3 These results therefore confirm that activity of the Bcl6 oncogene restricted to HS/ PCs can induce malignancies in mice that are of a post-germinal center stage of differentiation. Bcl6 mediates suppression of target genes via recruitment of factors that epigenetically modify chromatin. We hypothesized that this may elicit changes in DNA methylation, a mark that is capable acting in gene silencing memory,
and therefore investigated whether this may be a potential mechanism for Bcl6medited “hit-and-run” oncogenesis. Using genome-wide DNA methylation profiling of populations of HS/PCs and mature B-cells from wild type and Sca1Bcl6 mice, we identified broad epigenetic changes associated with the expression of Bcl6 in HS/PCs. Importantly, a significant subset of these changes were found to be maintained from HS/PCs to mature B cells in Sca1–Bcl6 mice, resulting in these populations being epigenetically more similar to each other than to their comparative population from wild-type mice.3 Together these results suggest that the “hit-and-run” role of Bcl6 activity may be manifested via epigenetic alterations. This epigenetic memory would maintain gene expression states through cell generations without a change in DNA sequence and in the absence of initiating signals. In conclusion, we have provided the first evidence of a “hit-and-run” role for Bcl6 in the oncogenesis of lymphoma. The implication of these findings is that, if ABC-like DLBCL occurs via a reprogramming-like mechanism, oncogenes that initiate tumor formation might be dispensable for tumor cell survival and/or tumor progression. In this context, mutations that activate oncogenes would have a driving role in the reprogramming process, but may act as passenger mutations thereafter, or may have a secondary role in evolved tumor cell clones.5,6 This may provide an explanation for the failure of some modern targeted therapies to clear tumor stem cells, despite being effective agents
*Correspondence to: Ash A Alizadeh; Email: [email protected]; Isidro Sánchez-García; Email: [email protected] Submitted: 05/09/2014; Accepted: 05/12/2014; Published Online: 05/27/2014 http://dx.doi.org/10.4161/cc.29326 Comment on: Green MR, et al. Nat Commun 2014; 5:3904; PMID:24887457; http://dx.doi.org/10.1038/ncomms4904
www.landesbioscience.com
Cell Cycle 1831
Downloaded by [198.91.36.79] at 03:31 22 February 2015
in vitro and in vivo.7 If BCL6 was required to maintain lymphoma-repopulating stem cells, then such a BCL6-targeted therapy might indeed prove effective in eradicating residual tumor cells when translated to the human setting. On the other hand, the fact that BCL6 can also contribute to the malignant phenotype through a hitand-run mechanism dependent on aberrant epigenetic programming of HS/PCs raises the possibility that tumor cells could emerge that become BCL6-independent. This prediction is supported by the fact that certain BCL6-positive DLBCL cell lines such as OCI-Ly8 (which contains a BCL6 translocation) and Karpas 422 are not killed by BCL6 shRNA or BCL6targeted therapy. From the therapeutic standpoint, this would indicate the need to combine BCL6 inhibitors with drugs that can kill these BCL6-independent cells. These concepts are therefore central to the full understanding of disease genesis and critical for the development of modern therapies aimed at directly addressing such genetic lesions. References 1. 2.
3.
Figure 1. Bcl6 hit-and-run oncogenesis in lymphoma. Transient introduction of Bcl6 into HS/PCs was sufficient to induce ABC-DLBCL lymphoma through specific genetic/epigenetic changes in HS/PCs that do not interfere with normal B-cell development. This model initially applies to the mouse and the role of BCL6 in human DLBCL progenitors is still unknown and an area of future investigation.
4.
5.
6.
against evolved tumor cells. For instance, BCL6 has emerged as a potentially important therapeutic target in recent years
1832
given its frequent involvement in lymphomagenesis and the fact that specific BCL6 inhibitors can kill lymphoma cells
Cell Cycle
7.
Armitage JO, et al. J Clin Oncol 1998; 16:2780-95; PMID:9704731 Alizadeh AA, et al. Nature 2000; 403:50311; PMID:10676951; http://dx.doi. org/10.1038/35000501 Green MR, et al. Nat Commun 2014; 5:3904; PMID:24887457; http://dx.doi.org/10.1038/ ncomms4904 Cattoretti G, et al. Cancer Cell 2005; 7:445-55; PMID:15894265; http://dx.doi.org/10.1016/j. ccr.2005.03.037 Vicente-Dueñas C, et al. EMBO J 2013; 32:150213; PMID:23632857; http://dx.doi.org/10.1038/ emboj.2013.97 Shin K, et al. Nat Cell Biol 2014; 16:469-78; PMID:24747439; http://dx.doi.org/10.1038/ ncb2956 Cerchietti LC, et al. Blood 2009; 113:3397-405; PMID:18927431; http://dx.doi.org/10.1182/ blood-2008-07-168773
Volume 13 Issue 12
