å¡ ORIGINAL
ARTICLE
å¡
Histocompatibility Antigens Associated with Beh^et's Disease in Northern Han Chinese Satoru Mineshita**, Ding Tian*, Li-man Wang***, Xiu-Yi Jian****, Shi-Yin Li*, Gao-Zhang Fang*****, Tian-Yu Bian***, Hai-Sun Liao*, Mitsuru Tsuchida**, Heizo Tanaka** and Akira Sakuma** Among Han nationality Chinese and living in the northern area of the Yellow River, 120 patients suffering from Beh^et's disease and 100 unrelated healthy individuals were typed for histocompatibility antigens (HLA)-A, -B, -C, and -DR and -DQ antigens. HLA-DR and DQ typing was performed on B-lymphocyte separated with Lympho-B-Kwik. The HLA-antisera were provided by llth IHWC. Bf alleles and C4 allotypes were determined by immunofixation agarose-gel electrophoresis. HLA-B51 was found in 67/120 (55.83%) patients and in 12/100 (12%) controls,frequency the Chi-square and relativeincreased risk values 45.54 group. and 9.27, respectively < 0.0005). C4AQo was significantly in were the patient In the complete (p form group HLA-B51 was observed more frequently (62.79%). No significant differences of other HLA antigens, frequencies, Bf or B4 alleles were found between the groups. (Internal Medicine 31: 1073-1075, 1992) Key words: Beh^et's disease, HLA, Chinese
Introduction
Materials and Methods
included 120entity patients suffering from BD Beliefs diseaseThis (BD)study is a polymorphic charac(39 males and and 81 females, 19-63 yearseye old)and who were terized by oral genital ulcerations, skin lesions, arthritis arthralgias, thrombophlebitis, all of being Hanand/or Chinese nationality and living in theand diverse system involvement. northernvisceral area ofand the central Yellownervous River. The diagnosis of BD The exact etiopathogenesis of BD is still obscure although was made according to the Beliefs Syndrome Research partial explanations have been revealed. Since Ohno et Committee of Japan (6). On the basis of the diagnostic al reported a strong association between HLA-B5 and criteria, 43 patients classified the demonstrated complete form BD in 1973 (1), this were observation hasasbeen and 77 cases, thegroups incomplete The control in various ethnic (2-4),form. including Chinesegroup (5). In was themade course upof ofthis 100study unrelated of BD, healthy doctors individuals from Japan of doctors Han Chinese from various nationality, different who districts lived in the in China same collabregion HLA-A, -B andparticipating -Cthetyping was withand the orated analyze association between as the to patients in performed this study. BD histocompatibility antigens (HLA), in particular HLAstandard NIH microcytotoxicity assay. The HLA-DR III, of which there is a lack of data in Chinese patients. and -DQ were studied on B-lymphocyte separated with This study is significant due to the fact that data were Lympho-B-Kwik (One Lamda, U.S.A.). The HLA reconfirmed as precisely as possible by coworkers in antisera (set 2 or 1) were provided by llth-International two countries. Histocompatibility Workshop and Conference. For complement allotyping, Bf alleles and C4 allotypes were determined by immunofixation-agarosegel electro phoresis. Statistical analysis was performed by the From *the People's Hospital, Beijing Medical University (Xuanwu Hospital, Capital Institute of Medicine, Beijing 100053), **the Medical Research Institute, Tokyo Medical and Dental University, ***the Tianjing Integrated Traditional Chinese and Western Medicine Dermatological Institute, ****the Third Hospital, Beijing Medical University and *****the Second Hospital, Harbin Medical University Received for publication January 23, 1992; Accepted for publication June 15, 1992 Reprint requests should be addressed to Dr. Satoru Mineshita, the Department of Preventive Medicine, Tokyo Medical and Dental Univers MRI, 5-45, Yushima 1-chome, Bunkyo-ku, Tokyo 113, Japan Internal Medicine Vol. 31, No. 9 (September 1992) 1073
Mineshita
et al
Chi-square method for each antigen in the computer department of Beijing Blood Center.
Table 1. Relative HLA Antigen Frequence (AF) and Gene Frequence (GF) P atie n ts
C o n tr o ls
Results H LA -
HLA-B51 was found in 67/120 (55.83%) patients and in 12/100 (12%) controls, the Chi-square and relative risk (RR) values were 45.54 and 9.27 respectively (Table 1, p < 0.0005). In the complete form group, the frequency of HLA-B51 was higher (27/43, 62.79%, Table 2) than in the incomplete form (40/77, 51.95%), but there was no statistically significant difference. Some HLA anti gens, such as a cluster of -Bw52 and -B35, often showed strong cross reactions serologically with -B51 but their frequencies were not changed in the study. The frequency of HLA-DR4 and -DQw3 was slightly increased in the patient group but the differences were not statistically significant 1). No in the Table 3 lists(Table the results of significant C4A, C4B differences and Bf in the otherpatients BD HLA antigens and controls were respectively. found. As shown in this Table, Bf S07 frequency was increased in the patient group, but no statistical significance was found between the groups. C4AQ0 frequency was significantly increased in the patient group (x2 = 39.16; RR = 16.00, P
ARTICLE
å¡
Histocompatibility Antigens Associated with Beh^et's Disease in Northern Han Chinese Satoru Mineshita**, Ding Tian*, Li-man Wang***, Xiu-Yi Jian****, Shi-Yin Li*, Gao-Zhang Fang*****, Tian-Yu Bian***, Hai-Sun Liao*, Mitsuru Tsuchida**, Heizo Tanaka** and Akira Sakuma** Among Han nationality Chinese and living in the northern area of the Yellow River, 120 patients suffering from Beh^et's disease and 100 unrelated healthy individuals were typed for histocompatibility antigens (HLA)-A, -B, -C, and -DR and -DQ antigens. HLA-DR and DQ typing was performed on B-lymphocyte separated with Lympho-B-Kwik. The HLA-antisera were provided by llth IHWC. Bf alleles and C4 allotypes were determined by immunofixation agarose-gel electrophoresis. HLA-B51 was found in 67/120 (55.83%) patients and in 12/100 (12%) controls,frequency the Chi-square and relativeincreased risk values 45.54 group. and 9.27, respectively < 0.0005). C4AQo was significantly in were the patient In the complete (p form group HLA-B51 was observed more frequently (62.79%). No significant differences of other HLA antigens, frequencies, Bf or B4 alleles were found between the groups. (Internal Medicine 31: 1073-1075, 1992) Key words: Beh^et's disease, HLA, Chinese
Introduction
Materials and Methods
included 120entity patients suffering from BD Beliefs diseaseThis (BD)study is a polymorphic charac(39 males and and 81 females, 19-63 yearseye old)and who were terized by oral genital ulcerations, skin lesions, arthritis arthralgias, thrombophlebitis, all of being Hanand/or Chinese nationality and living in theand diverse system involvement. northernvisceral area ofand the central Yellownervous River. The diagnosis of BD The exact etiopathogenesis of BD is still obscure although was made according to the Beliefs Syndrome Research partial explanations have been revealed. Since Ohno et Committee of Japan (6). On the basis of the diagnostic al reported a strong association between HLA-B5 and criteria, 43 patients classified the demonstrated complete form BD in 1973 (1), this were observation hasasbeen and 77 cases, thegroups incomplete The control in various ethnic (2-4),form. including Chinesegroup (5). In was themade course upof ofthis 100study unrelated of BD, healthy doctors individuals from Japan of doctors Han Chinese from various nationality, different who districts lived in the in China same collabregion HLA-A, -B andparticipating -Cthetyping was withand the orated analyze association between as the to patients in performed this study. BD histocompatibility antigens (HLA), in particular HLAstandard NIH microcytotoxicity assay. The HLA-DR III, of which there is a lack of data in Chinese patients. and -DQ were studied on B-lymphocyte separated with This study is significant due to the fact that data were Lympho-B-Kwik (One Lamda, U.S.A.). The HLA reconfirmed as precisely as possible by coworkers in antisera (set 2 or 1) were provided by llth-International two countries. Histocompatibility Workshop and Conference. For complement allotyping, Bf alleles and C4 allotypes were determined by immunofixation-agarosegel electro phoresis. Statistical analysis was performed by the From *the People's Hospital, Beijing Medical University (Xuanwu Hospital, Capital Institute of Medicine, Beijing 100053), **the Medical Research Institute, Tokyo Medical and Dental University, ***the Tianjing Integrated Traditional Chinese and Western Medicine Dermatological Institute, ****the Third Hospital, Beijing Medical University and *****the Second Hospital, Harbin Medical University Received for publication January 23, 1992; Accepted for publication June 15, 1992 Reprint requests should be addressed to Dr. Satoru Mineshita, the Department of Preventive Medicine, Tokyo Medical and Dental Univers MRI, 5-45, Yushima 1-chome, Bunkyo-ku, Tokyo 113, Japan Internal Medicine Vol. 31, No. 9 (September 1992) 1073
Mineshita
et al
Chi-square method for each antigen in the computer department of Beijing Blood Center.
Table 1. Relative HLA Antigen Frequence (AF) and Gene Frequence (GF) P atie n ts
C o n tr o ls
Results H LA -
HLA-B51 was found in 67/120 (55.83%) patients and in 12/100 (12%) controls, the Chi-square and relative risk (RR) values were 45.54 and 9.27 respectively (Table 1, p < 0.0005). In the complete form group, the frequency of HLA-B51 was higher (27/43, 62.79%, Table 2) than in the incomplete form (40/77, 51.95%), but there was no statistically significant difference. Some HLA anti gens, such as a cluster of -Bw52 and -B35, often showed strong cross reactions serologically with -B51 but their frequencies were not changed in the study. The frequency of HLA-DR4 and -DQw3 was slightly increased in the patient group but the differences were not statistically significant 1). No in the Table 3 lists(Table the results of significant C4A, C4B differences and Bf in the otherpatients BD HLA antigens and controls were respectively. found. As shown in this Table, Bf S07 frequency was increased in the patient group, but no statistical significance was found between the groups. C4AQ0 frequency was significantly increased in the patient group (x2 = 39.16; RR = 16.00, P
