Food Chemistry 133 (2012) 489–496
Contents lists available at SciVerse ScienceDirect
Food Chemistry journal homepage: www.elsevier.com/locate/foodchem
Analytical Methods
High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column Wejdan Shakir Khayoon a, Bahruddin Saad a,⇑, Tien Ping Lee a, Baharuddin Salleh b a b
School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
a r t i c l e
i n f o
Article history: Received 29 March 2011 Received in revised form 5 November 2011 Accepted 10 January 2012 Available online 28 January 2012 Keywords: Aflatoxins High performance liquid chromatography Monolithic column Foodstuffs Liquid chromatography–tandem mass spectrometry
a b s t r a c t A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTEÒ multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7 min using ChromolithÒ performance RP-18e (100–4.6 mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38–104.5% and RSDs were
Contents lists available at SciVerse ScienceDirect
Food Chemistry journal homepage: www.elsevier.com/locate/foodchem
Analytical Methods
High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column Wejdan Shakir Khayoon a, Bahruddin Saad a,⇑, Tien Ping Lee a, Baharuddin Salleh b a b
School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
a r t i c l e
i n f o
Article history: Received 29 March 2011 Received in revised form 5 November 2011 Accepted 10 January 2012 Available online 28 January 2012 Keywords: Aflatoxins High performance liquid chromatography Monolithic column Foodstuffs Liquid chromatography–tandem mass spectrometry
a b s t r a c t A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTEÒ multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7 min using ChromolithÒ performance RP-18e (100–4.6 mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38–104.5% and RSDs were
