Short Communication
438
Figure. Plasma GH, IRI, FF A and blood glucose responses to arginine (solid lines) and to arginine + aminophylline (broken lines) in 10 normal women. The vertical bars indicate ± S.E. On the right side of the graph the individual and mean GH and IRI peak values are reported .
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References Cavagnini, F., M. Peracchi: J.Endocr. 51: 651 (1971). - Dole, V.P., H. Meinem: J.BioI.Chem. 235: 2595 (1960). - Efendic, S., E. Cerasi, R. Luft: J.elin.Endocr. 34: 67 (1972). - Ensinck, J. W., R. W. Stoll, C.c. Gale, R.J. Santen, J.L. Touber, R.H. WiIIÜlms: J.clin.Endocr. 31: 153 (1970). - Levine, R.A., S. Oyama, A. Kagan, S,M. Glick: J.Lab. clin.Med. 75: 30 (1970). - Marco, J.• M. DÜlZ Fierros, I.M. Baroja, M.L. Vil/anueva, I. Valverde: Diabetes 21: 289 (1972). - Peake. G.T., A.L. Steiner, W.H. Daughaday: in Frontiers in neuroendocrinology. Eds. W.F. Ganong and L. Martini. Oxford University Press, New York p. 173 (1973). - Peracchi, M., F. Cavagnini. A.E. Pontiroli, V. Ra"i. A. Malinverni, M. Pinto: Acta Endocr. 76: 488 (1974). - Strauch. G.• E. ModiglÜlni, H. Bricaire: J.clin.Endocr. 29: 606 (1969). - Turtle. J.A., D.M. Kipnis: Biochem.biophys.Res.Commun. 28: 797 (1967). -
Requests for reprints should be addressed to: M. Peracchi, Clinica Medica I, via F.Sforza 35, 1-20122 Milano (ltaly)
Horm. Metab. Res. 7 (1975) 438-439
© Georg Thieme Verlag Stuttgart Growth Hormone and Insulin Secretion in Oophoreetomized Wornen
Downloaded by: East Carolina University. Copyrighted material.
i- "Of.. _
Comment We have recently suggested that aminophylline, in vivo, increases hypothalamic cAMP concentration and thereby probably simulates a ß,adre,nergic stimulation which inhibits GH response to hypoglycaemia (Pe· racchi et al. 1974). Failure of aminophylline to modify GH response to arginine could be explained if the adrenergic system is not involved in arginine-induced GH secretion (Strauch, ModiglÜlni and Bricaire 1969; Cavagnini and Peracchi 1971). In agreement with the data of Efendic, Cerasi and Luft (1972) and of Marco et al. (1972) the present study indicates that aminophylline significantly enhances the IRI response to arginine infusion in normal subjects. This finding reproduces the effect on IRI secretion of a ß,adrenergic stimulation whieh is known to aet through an increase of intracellular cAMP concentration (Turtle and Kipnis 1967).
E. Syvälahti, R. Erkkola, E. Iisalo, R. Punnonen and L. Rauramo Department of Pharmacology and Department of Obstetrics and Gynecology. University of Turku. Turku. Finland
Glucose tolerance is impaired with increasing age. The role of hormonal Changes during the menopause is, however, obscure. After castration a striking release of gonadotrophic, thyrotropic and adrenotropic hormones probably occurs (Masters 1952). Growth hormone (GH) is an important factor regulating glucose metabolism, but the influence of castration on GH secretion in man is not known. In order to investigate the effect of menopausal decline of ovarian function on carbohydrate metabolism, we have studied the response of blood glucose, serum immunoreactive insulin (lRI) and serum GH to tolbutamide before and after castration. Material and Methods For gynecological reasons abilateral oophorectomy was performed on 12 patients (mean age 52 ± 1 (SEM) years, height 161 ± 2 cm, and weight 69 ± 3 kg). They were a11 menstruating and had no climacteric symptoms, and no familiar or anamnestic disturbances of glucose metabolism. On the preoperative day at 8.30 after an overnight fast 1 gm tolbutamide (Rastinon®, Hoechst) dissolved in 20 ml of saline was injected over a 3 minute period. Blood sampies were taken through an intravenous cannula at 0, 5, 30, 60, 90, 120 and 180 minutes. The test was repeated 5-6 weeks after the operation. Serum IRI and GH were determined using the immunosorbent radioimmunoassay method (Wide 1969). Standard preparation for GH was NIH-GH-HS 1394 and for IRI WHO (66/304) human insulin. Rec.: 30 Nov. 1974 Ace.: 18 June 1975
439
Short Communication Figure. Blood glucose and serum IRI and GH coneentrations (mean ing an intravenous tolbutamide test before and after castration.
.' % -
E
Results
~F
The fasting blood glucose was 86.2 ± 2.5 mg/iOO ml preoperatively and 87.7 ± 3.3 mg/iOO ml postoperatively. The ehanges in the blood glucose after tolbutamide were very similar in the two test occasions and there were no significant differenees between the two tests at any time point (Figure). The fasting serum IRI was preoperatively 10.7 ± 2.5IlU/ml and postoperatively 12.0 ± 3.6 IlU/ml. The postoperative response eurve is above the preoperative curve throughout the study, the difference being significant at 30 minutes (27.4 ± 5.6IlU/ml and 20.0 ± 4.6 IlU/ml) (p 0.05) (Figure). The basal serum GH was slightly but insignificantly higher after the operation than before it (6.3 ± 1.6 ng/ml and 3.6 ± 0.8 ng/ml), and the levels remained slightly although insignificantly higher postoperatively (Figure).
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438
Figure. Plasma GH, IRI, FF A and blood glucose responses to arginine (solid lines) and to arginine + aminophylline (broken lines) in 10 normal women. The vertical bars indicate ± S.E. On the right side of the graph the individual and mean GH and IRI peak values are reported .
...::..000
.:
.. 100
"
... A""
_
llO~~ ,,.
.
,
,I-
0+
pcooo,·
'
I
~
+. I
~ ,
"
.........
" .0
o
0
'0
~
30
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~
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.1. T
•• &
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..
References Cavagnini, F., M. Peracchi: J.Endocr. 51: 651 (1971). - Dole, V.P., H. Meinem: J.BioI.Chem. 235: 2595 (1960). - Efendic, S., E. Cerasi, R. Luft: J.elin.Endocr. 34: 67 (1972). - Ensinck, J. W., R. W. Stoll, C.c. Gale, R.J. Santen, J.L. Touber, R.H. WiIIÜlms: J.clin.Endocr. 31: 153 (1970). - Levine, R.A., S. Oyama, A. Kagan, S,M. Glick: J.Lab. clin.Med. 75: 30 (1970). - Marco, J.• M. DÜlZ Fierros, I.M. Baroja, M.L. Vil/anueva, I. Valverde: Diabetes 21: 289 (1972). - Peake. G.T., A.L. Steiner, W.H. Daughaday: in Frontiers in neuroendocrinology. Eds. W.F. Ganong and L. Martini. Oxford University Press, New York p. 173 (1973). - Peracchi, M., F. Cavagnini. A.E. Pontiroli, V. Ra"i. A. Malinverni, M. Pinto: Acta Endocr. 76: 488 (1974). - Strauch. G.• E. ModiglÜlni, H. Bricaire: J.clin.Endocr. 29: 606 (1969). - Turtle. J.A., D.M. Kipnis: Biochem.biophys.Res.Commun. 28: 797 (1967). -
Requests for reprints should be addressed to: M. Peracchi, Clinica Medica I, via F.Sforza 35, 1-20122 Milano (ltaly)
Horm. Metab. Res. 7 (1975) 438-439
© Georg Thieme Verlag Stuttgart Growth Hormone and Insulin Secretion in Oophoreetomized Wornen
Downloaded by: East Carolina University. Copyrighted material.
i- "Of.. _
Comment We have recently suggested that aminophylline, in vivo, increases hypothalamic cAMP concentration and thereby probably simulates a ß,adre,nergic stimulation which inhibits GH response to hypoglycaemia (Pe· racchi et al. 1974). Failure of aminophylline to modify GH response to arginine could be explained if the adrenergic system is not involved in arginine-induced GH secretion (Strauch, ModiglÜlni and Bricaire 1969; Cavagnini and Peracchi 1971). In agreement with the data of Efendic, Cerasi and Luft (1972) and of Marco et al. (1972) the present study indicates that aminophylline significantly enhances the IRI response to arginine infusion in normal subjects. This finding reproduces the effect on IRI secretion of a ß,adrenergic stimulation whieh is known to aet through an increase of intracellular cAMP concentration (Turtle and Kipnis 1967).
E. Syvälahti, R. Erkkola, E. Iisalo, R. Punnonen and L. Rauramo Department of Pharmacology and Department of Obstetrics and Gynecology. University of Turku. Turku. Finland
Glucose tolerance is impaired with increasing age. The role of hormonal Changes during the menopause is, however, obscure. After castration a striking release of gonadotrophic, thyrotropic and adrenotropic hormones probably occurs (Masters 1952). Growth hormone (GH) is an important factor regulating glucose metabolism, but the influence of castration on GH secretion in man is not known. In order to investigate the effect of menopausal decline of ovarian function on carbohydrate metabolism, we have studied the response of blood glucose, serum immunoreactive insulin (lRI) and serum GH to tolbutamide before and after castration. Material and Methods For gynecological reasons abilateral oophorectomy was performed on 12 patients (mean age 52 ± 1 (SEM) years, height 161 ± 2 cm, and weight 69 ± 3 kg). They were a11 menstruating and had no climacteric symptoms, and no familiar or anamnestic disturbances of glucose metabolism. On the preoperative day at 8.30 after an overnight fast 1 gm tolbutamide (Rastinon®, Hoechst) dissolved in 20 ml of saline was injected over a 3 minute period. Blood sampies were taken through an intravenous cannula at 0, 5, 30, 60, 90, 120 and 180 minutes. The test was repeated 5-6 weeks after the operation. Serum IRI and GH were determined using the immunosorbent radioimmunoassay method (Wide 1969). Standard preparation for GH was NIH-GH-HS 1394 and for IRI WHO (66/304) human insulin. Rec.: 30 Nov. 1974 Ace.: 18 June 1975
439
Short Communication Figure. Blood glucose and serum IRI and GH coneentrations (mean ing an intravenous tolbutamide test before and after castration.
.' % -
E
Results
~F
The fasting blood glucose was 86.2 ± 2.5 mg/iOO ml preoperatively and 87.7 ± 3.3 mg/iOO ml postoperatively. The ehanges in the blood glucose after tolbutamide were very similar in the two test occasions and there were no significant differenees between the two tests at any time point (Figure). The fasting serum IRI was preoperatively 10.7 ± 2.5IlU/ml and postoperatively 12.0 ± 3.6 IlU/ml. The postoperative response eurve is above the preoperative curve throughout the study, the difference being significant at 30 minutes (27.4 ± 5.6IlU/ml and 20.0 ± 4.6 IlU/ml) (p 0.05) (Figure). The basal serum GH was slightly but insignificantly higher after the operation than before it (6.3 ± 1.6 ng/ml and 3.6 ± 0.8 ng/ml), and the levels remained slightly although insignificantly higher postoperatively (Figure).
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