RESEARCH ARTICLE DOI: 10.4274/tjh.2014.0181 Turk J Hematol 2016;33:28-33

Freezing of Apheresis Platelet Concentrates in 6% Dimethyl Sulfoxide: The First Preliminary Study in Turkey Aferez Trombosit Konsantrelerinin Yüzde 6’lık Dimetil Sülfoksitte Dondurulması: İlk Türkiye Çalışmasının Başlatılması Soner Yılmaz1, Rıza Aytaç Çetinkaya1, İbrahim Eker2, Aytekin Ünlü3, Metin Uyanık4, Serkan Tapan4, Ahmet Pekoğlu1, Aysel Pekel5, Birgül Erkmen6, Uğur Muşabak5, Sebahattin Yılmaz1, İsmail Yaşar Avcı7, Ferit Avcu6, Emin Kürekçi2, Can Polat Eyigün7 1Gülhane Military Medical Academy, Blood Training Center and Blood Bank, Ankara, Turkey 2Gülhane Military Medical Academy, Division of Pediatric Hematology, Ankara, Turkey 3Gülhane Military Medical Academy, Department of General Surgery, Ankara, Turkey 4Gülhane Military Medical Academy, Department of Medical Biochemistry, Ankara, Turkey 5Gülhane Military Medical Academy, Division of Immunology and Allergy, Ankara, Turkey 6Gülhane Military Medical Academy, Division of Hematology, Ankara, Turkey 7Gülhane Military Medical Academy, Department of Infectious Disease and Clinical Microbiology, Ankara, Turkey

Abstract

Öz

Objective: Transfusion of platelet suspensions is an essential part of patient care for certain clinical indications. In this pioneering study in Turkey, we aimed to assess the in vitro hemostatic functions of platelets after cryopreservation. Materials and Methods: Seven units of platelet concentrates were obtained by apheresis. Each apheresis platelet concentrate (APC) was divided into 2 equal volumes and frozen with 6% dimethyl sulfoxide (DMSO). The 14 frozen units of APCs were kept at -80 °C for 1 day. APCs were thawed at 37 °C and diluted either with autologous plasma or 0.9% NaCl. The volume and residual numbers of leukocytes and platelets were tested in both before-freezing and post-thawing periods. Aggregation and thrombin generation tests were used to analyze the in vitro hemostatic functions of platelets. Flow-cytometric analysis was used to assess the presence of frozen treated platelets and their viability. Results: The residual number of leukocytes in both dilution groups was

Freezing of Apheresis Platelet Concentrates in 6% Dimethyl Sulfoxide: The First Preliminary Study in Turkey.

Amaç: Trombosit süspansiyonlarının transfüzyonu, belirli klinik endikasyonlarda hastaların tedavisinin önemli bir parçasıdır. Bu çalışma ile Türkiye’d...
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