RESEARCH ARTICLE
Fluctuations of Serum Neuron Specific Enolase and Protein S-100B Concentrations in Relation to the Use of Shunt during Carotid Endarterectomy Marko Dragas1,2*, Igor Koncar1,2, Dragan Opacic3, Nikola Ilic1,2, Zivan Maksimovic1,2, Miroslav Markovic1,2, Marko Ercegovac2,4, Tatjana Simic2,5, Marija Pljesa-Ercegovac2,5, Lazar Davidovic1,2 1 Clinic for Vascular and Endovascular Surgery, Clinical Centre of Serbia, Belgrade, Serbia, 2 Faculty of Medicine, University of Belgrade, Belgrade, Serbia, 3 Faculty of Health, Medicine and Life Sciences, Department of Physiology, Maastricht University, Maastricht, The Netherlands, 4 Clinic of Neurology, Clinical Centre of Serbia, Belgrade, Serbia, 5 Institute of Medical and Clinical Biochemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia * [email protected]
OPEN ACCESS Citation: Dragas M, Koncar I, Opacic D, Ilic N, Maksimovic Z, Markovic M, et al. (2015) Fluctuations of Serum Neuron Specific Enolase and Protein S100B Concentrations in Relation to the Use of Shunt during Carotid Endarterectomy. PLoS ONE 10(4): e0124067. doi:10.1371/journal.pone.0124067 Academic Editor: Marco Gemma, Scientific Inst. S. Raffaele Hosp., ITALY Received: November 17, 2014 Accepted: February 25, 2015 Published: April 10, 2015 Copyright: © 2015 Dragas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: Presented study is a part of a scientific research project (Grant OI175008) supported by the Ministry of Education and Science of the Republic of Serbia, MD NI ZM MM LD. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist.
Abstract Objective To evaluate the changes in serum neuron specific enolase and protein S-100B, after carotid endarterectomy performed using the conventional technique with routine shunting and patch closure, or eversion technique without the use of shunt.
Materials and Methods Prospective non-randomized study included 43 patients with severe (>80%) carotid stenosis undergoing carotid endarterectomy in regional anesthesia. Patients were divided into two groups: conventional endarterectomy with routine use of shunt and Dacron patch (csCEA group) and eversion endarterectomy without the use of shunt (eCEA group). Protein S-100B and NSE concentrations were measured from peripheral blood before carotid clamping, after declamping and 24 hours after surgery.
Results Neurologic examination and brain CT findings on the first postoperative day did not differ from preoperative controls in any patients. In csCEA group, NSE concentrations decreased after declamping (P
Fluctuations of Serum Neuron Specific Enolase and Protein S-100B Concentrations in Relation to the Use of Shunt during Carotid Endarterectomy Marko Dragas1,2*, Igor Koncar1,2, Dragan Opacic3, Nikola Ilic1,2, Zivan Maksimovic1,2, Miroslav Markovic1,2, Marko Ercegovac2,4, Tatjana Simic2,5, Marija Pljesa-Ercegovac2,5, Lazar Davidovic1,2 1 Clinic for Vascular and Endovascular Surgery, Clinical Centre of Serbia, Belgrade, Serbia, 2 Faculty of Medicine, University of Belgrade, Belgrade, Serbia, 3 Faculty of Health, Medicine and Life Sciences, Department of Physiology, Maastricht University, Maastricht, The Netherlands, 4 Clinic of Neurology, Clinical Centre of Serbia, Belgrade, Serbia, 5 Institute of Medical and Clinical Biochemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia * [email protected]
OPEN ACCESS Citation: Dragas M, Koncar I, Opacic D, Ilic N, Maksimovic Z, Markovic M, et al. (2015) Fluctuations of Serum Neuron Specific Enolase and Protein S100B Concentrations in Relation to the Use of Shunt during Carotid Endarterectomy. PLoS ONE 10(4): e0124067. doi:10.1371/journal.pone.0124067 Academic Editor: Marco Gemma, Scientific Inst. S. Raffaele Hosp., ITALY Received: November 17, 2014 Accepted: February 25, 2015 Published: April 10, 2015 Copyright: © 2015 Dragas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: Presented study is a part of a scientific research project (Grant OI175008) supported by the Ministry of Education and Science of the Republic of Serbia, MD NI ZM MM LD. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist.
Abstract Objective To evaluate the changes in serum neuron specific enolase and protein S-100B, after carotid endarterectomy performed using the conventional technique with routine shunting and patch closure, or eversion technique without the use of shunt.
Materials and Methods Prospective non-randomized study included 43 patients with severe (>80%) carotid stenosis undergoing carotid endarterectomy in regional anesthesia. Patients were divided into two groups: conventional endarterectomy with routine use of shunt and Dacron patch (csCEA group) and eversion endarterectomy without the use of shunt (eCEA group). Protein S-100B and NSE concentrations were measured from peripheral blood before carotid clamping, after declamping and 24 hours after surgery.
Results Neurologic examination and brain CT findings on the first postoperative day did not differ from preoperative controls in any patients. In csCEA group, NSE concentrations decreased after declamping (P
