Theor Appl Genet DOI 10.1007/s00122-015-2663-1

ORIGINAL ARTICLE

Fine mapping of barley locus Rps6 conferring resistance to wheat stripe rust Kun Li1,2 · Joshua Hegarty2 · Chaozhong Zhang1 · Anmin Wan3 · Jiajie Wu1 · Gina Brown Guedira4 · Xianming Chen3,5 · María Muñoz‑Amatriaín6 · Daolin Fu1 · Jorge Dubcovsky2,7 

Received: 10 August 2015 / Accepted: 22 December 2015 © The Author(s) 2016. This article is published with open access at Springerlink.com

Abstract  Key message  Barley resistance to wheat stripe rust has remained effective for along time and, therefore, the genes underlying this resistance can be a valuable tool toengineer durable resistance in wheat. Abstract  Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major disease of wheat that is causing large economic losses in many wheat-growing regions of the world. Deployment of Pst resistance genes has been an effective strategy for controlling this pathogen, but many of

Communicated by K. Smith. D. Fu and J. Dubcovsky contributed equally to this work. * Daolin Fu [email protected] * Jorge Dubcovsky [email protected] 1

State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong, China

2

Department of Plant Sciences, University of California, Davis, CA 95616, USA

3

Department of Plant Pathology, Washington State University, Pullman, WA 99164, USA

4

USDA‑ARS, Plant Science Research Unit, Department of Crop Science, North Carolina State University, Raleigh, NC 27695, USA

5

USDA-ARS, Wheat Genetics, Quality, Physiology, and Disease Research Unit, Pullman, WA 99164, USA

6

Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA

7

Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA







these genes have been defeated by new Pst races. In contrast, genes providing resistance to this wheat pathogen in other grass species (nonhost resistance) have been more durable. Barley varieties (Hordeum vulgare ssp. vulgare) are predominately immune to wheat Pst, but we identified three accessions of wild barley (Hordeum vulgare ssp. spontaneum) that are susceptible to Pst. Using these accessions, we mapped a barley locus conferring resistance to Pst on the distal region of chromosome arm 7HL and designated it as Rps6. The detection of the same locus in the cultivated barley ‘Tamalpais’ and in the Chinese barley ‘Y12’ by an allelism test suggests that Rps6 may be a frequent component of barley intermediate host resistance to Pst. Using a high-density mapping population (>10,000 gametes) we precisely mapped Rps6 within a 0.14 cM region (~500 kb contig) that is colinear to regions in Brachypodium (

Fine mapping of barley locus Rps6 conferring resistance to wheat stripe rust.

Barley resistance to wheat stripe rust has remained effective for a long time and, therefore, the genes underlying this resistance can be a valuable t...
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