Annals of Tropical Medicine & Parasitology
ISSN: 0003-4983 (Print) 1364-8594 (Online) Journal homepage: http://www.tandfonline.com/loi/ypgh19
Failure to immunise mice against Schistosoma mansoni by therapeutic eradication of the adult worm burden D. Gold & J. Lengy To cite this article: D. Gold & J. Lengy (1975) Failure to immunise mice against Schistosoma mansoni by therapeutic eradication of the adult worm burden, Annals of Tropical Medicine & Parasitology, 69:2, 265-266, DOI: 10.1080/00034983.1975.11687009 To link to this article: http://dx.doi.org/10.1080/00034983.1975.11687009
Published online: 15 Mar 2016.
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Date: 15 August 2017, At: 20:12
Annals of Tropical Medicine and Parisitology, 1975, Vol. 69, No. 2
SHORT COMMUNICATION
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Failure to immunise mice against Schistosoma mansoni by therapeutic eradication of the adult worm burden Kendall and Sinclair (1969) have demonstrated that hexachlorophane is capable of significantly protecting rabbits against infection with Fasciola hepatica if given 6-24 hours before oral administration of the metacercariae. Subsequently, Kendall and Sinclair (1971) showed that no resistance to a second infection is engendered in rabbits which have experienced a primary infection of Fasciola hepatica. However, if the first infection is terminated with hexachlorophane two days before the second infection is administered, significantly fewer flukes are recovered from this challenge infection. They concluded that the interaction of the previous infection and the drug adversely affected the environment of the invading flukes of the challenge infection. In the light of these results, an attempt was made to determine whether mice, in which a Schistosoma mansoni infection was terminated by Ambilhar* (Niridazole), would be rendered resistant to a challenge infection made immediately or shortly after administration of anthelmintic. TABLE
Summary of attempts to confer resistance to reinfection with S. mansoni in mice in which the initial infection was terminated by Ambilhar
Groups
Exp.A Exp. B Control! Control2 Control 3 Control4 Control 5
Number of mice
Protocol of treatment
Mean live worm burden
25 25 10 10 10 10 10
primary infection - chemotherapy - immediate challenge primary infection - chemotherapy - challenge one day later primary infection- sham chemotherapy- immediate challenge primary infection only challenge infection only primary infection- chemotherapy chemotherapy- immediate challenge
20.0 23.6 36.8 23.4 18.5 0.0 21.2
Two experimental groups each of 25 mice (Swiss strain), and five appropriate control groups each of 10 mice were used. Primary andfor challenge infections were made by exposing the mice individually to 100 S. mansoni cercariae (Egyptian strain) by the percutaneous body-immersion method. Primary infections were terminated at 60 days by treatment with Ambilhar*, 100 mgfkg twice daily for seven days, given by gavage. Sham chemotherapy was performed in one control group by the administration of tap water instead of the drug. All mice were killed 40 days after the challenge infection (or the specified treatment) and the live worm burden determined following perfusion of the liver and mesenteric vessels. The results obtained are summarized in the Table: no protection • Ambilhar supplied by Dr. H. P. Striebel, Ciba-Geigy Limited, Basle, Switzerland.
265
266 against the challenge infection occurred following drug administration. A corollary finding was that a 60-day primary infection with S. mansoni in mice did not confer any significant protection against a challenge infection.
D. GoLD ]. LENGY
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Received 3 January 1975
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. REFERENCES
KENDALL, S. KENDALL, S.
B. & B. &
SINCLAIR, SINCLAIR,
I. I.
J. (1969). Research in Veterinary Science, 10, 481-482. J. (1971). Research in Veterinary Science, 12, 74-79.