Am J Transl Res 2015;7(12):2543-2560 www.ajtr.org /ISSN:1943-8141/AJTR0017386
Original Article Extracorporeal shock wave therapy effectively prevented diabetic neuropathy Yi-Ling Chen1*, Kuan-Hung Chen2*, Tsung-Cheng Yin3, Tien-Hung Huang1, Chun-Man Yuen4, Sheng-Ying Chung1, Pei-Hsun Sung1, Meng-Shen Tong1, Chih-Hung Chen5, Hsueh-Wen Chang6, Kun-Chen Lin2, Sheung-Fat Ko7†, Hon-Kan Yip1,8,9,10,11† 1 Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; Departments of 2Anesthesiology, 3Orthopaedic Surgery, 7Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 4Division of Neurosurgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 5Divisions of General Medicine, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 6Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan; 8Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 9Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 10Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan; 11Department of Nursing, Asia University, Taichung, 41354, Taiwan. *Equal contributors. †Equal contributors.
Received October 6, 2015; Accepted November 18, 2015; Epub December 15, 2015; Published December 30, 2015 Abstract: Background: We tested the hypothesis that extracorporeal shock wave (ECSW) therapy can effectively protect sciatic nerve (SN) from diabetes mellitus (DM)-induced neuropathy in leptin-deficient (ob/ob) mice. Methods and results: Eighteen-week C57BL/6 mice (n=8) served as age-matched controls (group 1) and ob/ob mice (n=16) were categorized into DM (group 2) and DM + ECSW (0.12 mJ/mm2 for 4 times of 200 impulses at 3-week intervals) (group 3). The animals were sacrificed two weeks post-ECSW. In vitro results showed that the protein expressions of oxidative stress (NOX-1, NOX-2, oxidized protein), inflammation (MMP-9, TNF-α, iNOS), apoptosis (Bax, cleaved caspase-3, & PARP), and DNA-damage marker (γ-H2AX) were significantly higher in RT4-D6P2T (schwannoma cell line) treated by menadione (25 µM) compared with control group and were significantly reversed after ECSW (0.12 mJ/mm2, 200 impulses) (all p
Original Article Extracorporeal shock wave therapy effectively prevented diabetic neuropathy Yi-Ling Chen1*, Kuan-Hung Chen2*, Tsung-Cheng Yin3, Tien-Hung Huang1, Chun-Man Yuen4, Sheng-Ying Chung1, Pei-Hsun Sung1, Meng-Shen Tong1, Chih-Hung Chen5, Hsueh-Wen Chang6, Kun-Chen Lin2, Sheung-Fat Ko7†, Hon-Kan Yip1,8,9,10,11† 1 Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; Departments of 2Anesthesiology, 3Orthopaedic Surgery, 7Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 4Division of Neurosurgery, Department of Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 5Divisions of General Medicine, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 6Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan; 8Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 9Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 83301, Taiwan; 10Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan; 11Department of Nursing, Asia University, Taichung, 41354, Taiwan. *Equal contributors. †Equal contributors.
Received October 6, 2015; Accepted November 18, 2015; Epub December 15, 2015; Published December 30, 2015 Abstract: Background: We tested the hypothesis that extracorporeal shock wave (ECSW) therapy can effectively protect sciatic nerve (SN) from diabetes mellitus (DM)-induced neuropathy in leptin-deficient (ob/ob) mice. Methods and results: Eighteen-week C57BL/6 mice (n=8) served as age-matched controls (group 1) and ob/ob mice (n=16) were categorized into DM (group 2) and DM + ECSW (0.12 mJ/mm2 for 4 times of 200 impulses at 3-week intervals) (group 3). The animals were sacrificed two weeks post-ECSW. In vitro results showed that the protein expressions of oxidative stress (NOX-1, NOX-2, oxidized protein), inflammation (MMP-9, TNF-α, iNOS), apoptosis (Bax, cleaved caspase-3, & PARP), and DNA-damage marker (γ-H2AX) were significantly higher in RT4-D6P2T (schwannoma cell line) treated by menadione (25 µM) compared with control group and were significantly reversed after ECSW (0.12 mJ/mm2, 200 impulses) (all p
