AJEBAK 53 (Pt. 4) 251-256 (1975)

EVIDENCE EOR RECOMBINATION BETWEEN TWO DIFFERENT IMMUNOLOCICAL TYPES OF FOOT-AND-MOUTH DISEASE VIRUS by J. S. MACKENZIE" AND W . R. S L A D E (From the Cenetics Department, Animal Virus Research Institute, Pirbright, Woking, Surrey, U.K.) (Accepted for publication August 18, 1975.) Summary. Recombination was observed between temperature-sensitive (ts) mutants of two immunologieal types of foot-and-mouth disease vims which were distinguishable by two marker characteristics in addition to their antigenie type. Putative ts+ recombinants were isolated and the segregation patterns of their marker characteristics examined. The results are discussed in temis of the origin of new sub-type strains.

INTRODUCTION. Seven distinct serological and immunologieal types of foot-and-mouth disease virus (FMDV) are known, each of which can be further divided into numerous sub-types. Recombination has been observed between mutants isolated from a single strain of FMDV (Pringle, 1968; Mackenzie et al, 1975), and at a low frequency, between different sub-type strains belonging to the same immunologieal type (Pringle, 1965; Pringle and Slade, 1968). Recombination could not be detected, however, between different immunologieal types (Pringle and Slade, 1968). Using cross-hybridization techniques, Dietzschold et al. (1971) were able to demonstrate significant genetic homology between three different immunologieal types of FMDV which had been isolated in Europe. This finding, which was consistent with the antigenie relationships observed in experiments with cross-neutralization (Wittmann, 1966) and precipitation (Brown, Cartwright and Newman, 1964), would suggest that sufficient genetic homology might exist between different immunologieal types to permit a low level of recombination to occur. This report describes preliminary experimental evidence to substantiate such recombination between temperature-sensitive mutants isolated from two immunologieal types of FMDV. "Present address: Department of Microbiology, University of Western Australia, Perth Medical Centre, Shenton Park, Western Australia 6008.

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Evidence for recombination between two different immunological types of foot-and-mouth disease virus.

AJEBAK 53 (Pt. 4) 251-256 (1975) EVIDENCE EOR RECOMBINATION BETWEEN TWO DIFFERENT IMMUNOLOCICAL TYPES OF FOOT-AND-MOUTH DISEASE VIRUS by J. S. MACKEN...
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