BIOLOGY
OF
REPRODUCTION
Evidence
for
46,
315-321
Declining Uterine
(1992)
Extracellular Calcium Arterial Smooth Muscle and
D. B. FARLEY3 Department
of Ob/Gyn,3
Department
of Animal
Uptake during
C Activity
in
S. P. FORD24
University
Science,4
and Protein Kinase Gestation in Gilts”
of Iowa,
Iowa
State
Iowa
City,
University,
Iowa
Ames,
52242
Iowa
50011
ABSTRACT Uterine
arterial
gestation. smooth
KCI.
and
The
these
same -0.93;
=
kinase
induce
declines
(p
were
take
up
the
PKM
the
C (P1(C).
progressively with
arteries estrous
progressively
extracellular
during
cycle through
Day
p
as gestation
blood flow of gestation
creasing
production
responding
increases in the
increases
observed,
the
sensitivity circulating
to short-term catecholamines
contraction
uterine
enters
dominantly
the
through
channels larization
two
receptor
maternal
stress
ine
arterial
diameter
laboratory
[5]
numbers
and
tro-perfused
of
agonists
are
and
2)
1)
activated
by
flow
data
and
with
responses
of vascular
the
but
not
systemic
Received
Fehruar’
9.
Grant
11,
1991.
Vniversin.
and Home Economics research was supported
Stua3’
Exin
S. P. Ford. Ames.
of the
uterine
P1CC.
reduction
in Ca2
participation
of
kinase
of the
arterial
PKC
as an
phase
muscle of
up-
Ca2”-acti-
(PKC)
sustained
smooth
the activity changes in specific
in gilts,
activity
and
observed
of
110, 11].
PKC results in kinase (PKM), in coordinating [12-14]. that incycle and declines
activity.
Ca2’
of PKC. Study uterine arterial
uptakes
I was concontractile
in response
to
KCI-
to relate
these
in Study
1.
AND
changes
to changes
in
METHODS
IA 50011.
Department FAX
(515)
of Animal
Science.
II
Kildee
Halt,
1
Animals.
HD06380.
2Correspondence: State
protein
1991.
iournal Paper No, J-14383 of the Iowa Agriculture periment Station, Ames, IA; proiects 2443 and 2444; this
part by NIH
and
was that Ca2 uptake through the changes in uterine arterial diam-
MATERIALS October
uptake
as protein phosphorylation et al. [15] demonstrated
hypothesis long-term
and
gestation PSC
Accepted
in
5Ca’
advancement
protein
contractile
of by
induced membrane depolarization throughout gestation in guts. Study 2 was conducted to quantify the PKC and PKM activity of uterine arterial smooth muscle during the estrous cycle and the first, second, and third trimesters of
throughout
progressively
a selective
is indicated. emphasizes mediator
20,
uptake
cytosolic
intracellular
properties
of in vi-
in prolonged [9]. Because uterine
flow
the
ability
decreasing
important
eter by modulating ducted to evaluate
our
PSC results
increases
PSC
Days dose
PKC
with
phospholipid-dependent
Our overall PSC mediates
receptor
constant
arterial
declined
of gestation,
on
concentrations
a decreasing
with
through the Recent evidence
in uterine
in uterfrom
suggest
guts
creased estrogen production during the estrous late gestation in ewes is associated with significant
depodur-
a1-adrenergic
remain of
data
in association
(tone),
extracellular
in uterine PKC
cellular responses such Furthermore, Magness
pre-
channels:
contraction
arteries
[6].
cells
reductions
Previous
that
in pigs. The activation in vessel diameter
changes
flow
45Ca2
Estrogen and
labora.
and
a depolarizing
extracellular
0.01)
OF
REPRODUCTION
Evidence
for
46,
315-321
Declining Uterine
(1992)
Extracellular Calcium Arterial Smooth Muscle and
D. B. FARLEY3 Department
of Ob/Gyn,3
Department
of Animal
Uptake during
C Activity
in
S. P. FORD24
University
Science,4
and Protein Kinase Gestation in Gilts”
of Iowa,
Iowa
State
Iowa
City,
University,
Iowa
Ames,
52242
Iowa
50011
ABSTRACT Uterine
arterial
gestation. smooth
KCI.
and
The
these
same -0.93;
=
kinase
induce
declines
(p
were
take
up
the
PKM
the
C (P1(C).
progressively with
arteries estrous
progressively
extracellular
during
cycle through
Day
p
as gestation
blood flow of gestation
creasing
production
responding
increases in the
increases
observed,
the
sensitivity circulating
to short-term catecholamines
contraction
uterine
enters
dominantly
the
through
channels larization
two
receptor
maternal
stress
ine
arterial
diameter
laboratory
[5]
numbers
and
tro-perfused
of
agonists
are
and
2)
1)
activated
by
flow
data
and
with
responses
of vascular
the
but
not
systemic
Received
Fehruar’
9.
Grant
11,
1991.
Vniversin.
and Home Economics research was supported
Stua3’
Exin
S. P. Ford. Ames.
of the
uterine
P1CC.
reduction
in Ca2
participation
of
kinase
of the
arterial
PKC
as an
phase
muscle of
up-
Ca2”-acti-
(PKC)
sustained
smooth
the activity changes in specific
in gilts,
activity
and
observed
of
110, 11].
PKC results in kinase (PKM), in coordinating [12-14]. that incycle and declines
activity.
Ca2’
of PKC. Study uterine arterial
uptakes
I was concontractile
in response
to
KCI-
to relate
these
in Study
1.
AND
changes
to changes
in
METHODS
IA 50011.
Department FAX
(515)
of Animal
Science.
II
Kildee
Halt,
1
Animals.
HD06380.
2Correspondence: State
protein
1991.
iournal Paper No, J-14383 of the Iowa Agriculture periment Station, Ames, IA; proiects 2443 and 2444; this
part by NIH
and
was that Ca2 uptake through the changes in uterine arterial diam-
MATERIALS October
uptake
as protein phosphorylation et al. [15] demonstrated
hypothesis long-term
and
gestation PSC
Accepted
in
5Ca’
advancement
protein
contractile
of by
induced membrane depolarization throughout gestation in guts. Study 2 was conducted to quantify the PKC and PKM activity of uterine arterial smooth muscle during the estrous cycle and the first, second, and third trimesters of
throughout
progressively
a selective
is indicated. emphasizes mediator
20,
uptake
cytosolic
intracellular
properties
of in vi-
in prolonged [9]. Because uterine
flow
the
ability
decreasing
important
eter by modulating ducted to evaluate
our
PSC results
increases
PSC
Days dose
PKC
with
phospholipid-dependent
Our overall PSC mediates
receptor
constant
arterial
declined
of gestation,
on
concentrations
a decreasing
with
through the Recent evidence
in uterine
in uterfrom
suggest
guts
creased estrogen production during the estrous late gestation in ewes is associated with significant
depodur-
a1-adrenergic
remain of
data
in association
(tone),
extracellular
in uterine PKC
cellular responses such Furthermore, Magness
pre-
channels:
contraction
arteries
[6].
cells
reductions
Previous
that
in pigs. The activation in vessel diameter
changes
flow
45Ca2
Estrogen and
labora.
and
a depolarizing
extracellular
0.01)
