Curr Med Mycol, 2015 Sep, 1(3): 25-32

‫ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ‬Original Article‫ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ‬ Evaluation of the antifungal activities of various extracts from Pistacia atlantica Desf Falahati M1, Sepahvand A2, Mahmoudvand H2,3, Baharvand P4, Jabbarnia S5, Ghojoghi A1, Yarahmadi M1,2* 1

Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran 3 Department of Medical Mycology and Parasitology, School of Medicine, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran 4 Department of Biostatistics, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran 5 Razi Vaccine and Serum Research Institute, Tehran, Iran 2

*Corresponding author: Mohammad Yarahmadi, Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Email: [email protected] (Received: 24 June 2015; Revised: 29 September 2015; Accepted: 27 October 2015)

Abstract Background and Purpose: Despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple treatment-related side-effects. The present study was designed to investigate the antifungal properties of different extracts from Pistacia atlantica Desf. Materials and Methods: Different parts of P. atlantica (i.e., dried fruit, fresh fruit and dried leaf) were separately extracted via percolation method with 80% methanol and water. Gas chromatography/mass spectrometry (GC/MS) analysis was performed to determine the main constituents of leaf and fruit extracts from P. atlantica. In vitro antiCandida activities of the extracts against Candida albicans, Candida glabrata and Saccharomyces cerevisiae were studied. For this purpose, the minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) were determined, using broth microdilution method, according to the modified M27-A3 protocol on yeasts, proposed by the Clinical and Laboratory Standards Institute (CLSI). Results: Based on GC/MS analysis, the main constituents of P. atlantica fruit extracts were β-myrcene (41.4%), αpinene (32.48%) and limonene (4.66%), respectively, whereas the major constituents of P. atlantica leaf extracts were trans-caryophyllene (15.18%), α-amorphene (8.1%) and neo-allo-ocimene (6.21%), respectively. As the findings indicated, all the constituents exhibited both fungistatic and fungicidal activities, with MICs ranging from 6.66 to 26.66 mg/mL and MFCs ranging from 13.3 to 37.3 mg/mL, respectively. Among the evaluated extracts, the methanolic fresh fruit extract of P. atlantica was significantly more effective than other extracts (P

Evaluation of the antifungal activities of various extracts from Pistacia atlantica Desf.

Despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple t...
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