Original Research Article
Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats Akram Ghadirkhomi1, Leila Safaeian2*, Behzad Zolfaghari3, Mohammad Reza Agha Ghazvini4, Parisa Rezaei5 1
Department of Pharmacology, Islamic Azad University, Shahreza Branch, Shahreza, Iran Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 3 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 4 Isfahan Center of Public Health Training and Research, Institute of Public Health Research, Tehran University of Medical Science, Iran 5 Department of Pathology, Seyed-Al-Shohada Hospital, Isfahan, Iran 2
Article history: Received: Oct 10, 2015 Received in revised form: Nov 18, 2015 Accepted: Dec 08, 2015 Vol. 6, No. 5, Sep-Oct 2016, 558-566. * Corresponding Author: Tel: +983137927087 Fax: +983136680011 [email protected] Keywords: Pinus eldarica Toxicity Hematology Serum biochemistry Histopathology
Abstract Objective: Pinus eldarica (P. eldarica) is one of the most common pines in Iran which has various bioactive constituents and different uses in traditional medicine. Since there is no documented evidence for P. eldarica safety, the acute and subacute oral toxicities of hydroalcoholic extract of P. eldarica bark were investigated in male and female Wistar rats in this study. Materials and Methods: In the acute study, a single dose of extract (2000 mg/kg) was orally administered and animals were monitored for 7 days. In the sub-acute study, repeated doses (125, 250 and 500 mg/kg/day) of the extract were administered for 28 days and biochemical, hematological and histopathological parameters were evaluated. Results: Our results showed no sign of toxicity and no mortality after single or repeated administration of P. eldarica. The median lethal dose (LD50) of P. eldarica was determined to be higher than 2000 mg/kg. The mean body weight and most of the biochemical and hematological parameters showed normal levels. There were only significant decreases in serum triglyceride levels at the doses of 250 and 500 mg/kg of the extract in male rats (p
Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats Akram Ghadirkhomi1, Leila Safaeian2*, Behzad Zolfaghari3, Mohammad Reza Agha Ghazvini4, Parisa Rezaei5 1
Department of Pharmacology, Islamic Azad University, Shahreza Branch, Shahreza, Iran Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 3 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 4 Isfahan Center of Public Health Training and Research, Institute of Public Health Research, Tehran University of Medical Science, Iran 5 Department of Pathology, Seyed-Al-Shohada Hospital, Isfahan, Iran 2
Article history: Received: Oct 10, 2015 Received in revised form: Nov 18, 2015 Accepted: Dec 08, 2015 Vol. 6, No. 5, Sep-Oct 2016, 558-566. * Corresponding Author: Tel: +983137927087 Fax: +983136680011 [email protected] Keywords: Pinus eldarica Toxicity Hematology Serum biochemistry Histopathology
Abstract Objective: Pinus eldarica (P. eldarica) is one of the most common pines in Iran which has various bioactive constituents and different uses in traditional medicine. Since there is no documented evidence for P. eldarica safety, the acute and subacute oral toxicities of hydroalcoholic extract of P. eldarica bark were investigated in male and female Wistar rats in this study. Materials and Methods: In the acute study, a single dose of extract (2000 mg/kg) was orally administered and animals were monitored for 7 days. In the sub-acute study, repeated doses (125, 250 and 500 mg/kg/day) of the extract were administered for 28 days and biochemical, hematological and histopathological parameters were evaluated. Results: Our results showed no sign of toxicity and no mortality after single or repeated administration of P. eldarica. The median lethal dose (LD50) of P. eldarica was determined to be higher than 2000 mg/kg. The mean body weight and most of the biochemical and hematological parameters showed normal levels. There were only significant decreases in serum triglyceride levels at the doses of 250 and 500 mg/kg of the extract in male rats (p
