Original Article

http://dx.doi.org/10.3349/ymj.2014.55.2.459 pISSN: 0513-5796, eISSN: 1976-2437

Yonsei Med J 55(2):459-466, 2014

Evaluation of a Commercial Glycoprotein Enzyme-Linked Immunosorbent Assay for Measuring Vaccine Immunity to Varicella Yun Hwa Kim,1 Ji Young Hwang,1 Hye Min Shim,1 Eunsil Lee,2 Songyong Park,3 and Hosun Park1 Departments of 1Microbiology and 2Pediatrics, College of Medicine, Yeungnam University, Daegu; Division of Biological Science and Technology, College of Science and Technology, Yonsei Univeristy, Wonju, Korea.

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Received: June 5, 2013 Revised: August 2, 2013 Accepted: August 9, 2013 Corresponding author: Dr. Hosun Park, Department of Microbiology, College of Medicine, Yeungnam University, 170 Hyeonchung-ro, Nam-gu, Daegu 705-703, Korea. Tel: 82-53-620-4364, Fax: 82-53-653-6628 E-mail: [email protected] ∙ The authors have no financial conflicts of interest.

Purpose: To evaluate a recently marketed commercial glycoprotein enzymelinked immunosorbent assay (gpEIA) kit, the VaccZymeTM VZV gpEIA, for measuring the immunity of varicella-vaccinated children. Materials and Methods: We investigated the accuracy and reproducibility of the VaccZymeTM VZV gpEIA kit for the detection of antibodies to VZV. We also examined the sensitivity, specificity, and correlation between antibody titers calculated with gpEIA versus fluorescent antibody to membrane antigen (FAMA) by using sera of 349 children, ranging from 1 to 6 years old. Results: VaccZymeTM VZV gpEIA gave precise and reproducible intra- and inter-assay results. FAMA and gpEIA titers showed a linear correlation (Pearson correlation coefficient=0.987). The sensitivity and specificity of the VaccZymeTM gpEIA was 31.4% and 100%, respectively, when the guidelines of the gpEIA (810 mIU/mL) were re-analyzed using the Screening kit. Procedures were followed according to the manufacturer’s instructions. According to the interpretation guidelines of the kit, an antibody titer

Evaluation of a commercial glycoprotein enzyme-linked immunosorbent assay for measuring vaccine immunity to varicella.

To evaluate a recently marketed commercial glycoprotein enzyme-linked immunosorbent assay (gpEIA) kit, the VaccZyme™ VZV gpEIA, for measuring the immu...
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