Path. Res. Pract. 187, 534-538 (1991)
Evaluation of 29 Monoclonal and Polyclonal Antibodies Used in the Diagnosis of Pituitary Adenomas A Collaborative Study from Pathologists of the Club Fran\ais de I'Hypophyse F. Labat-Moleur Laboratoire de Pathologie Gel/ulaire, Hopital Albert Michal/on, Grenoble
J. Trouillas Laboratoire d'Histologie et d'Embryologie, Faculte de Medecine Alexis Garrel, Lyon
D. Seret-Begue Departement de Pathologie, Hopital Lariboisiere, Paris
M. Kujas Departement de Pathologie, Hopital La Pitie-$alp{ltriere, Paris
M.-B. Delisle Departement de Pathologie, Hopital Rangueil, Toulouse
C. Ronin Laboratoire de Biochimie, Faculte Nord de Medecine, Marseille, France
SUMMARY Fifteen polyclonal antibodies (pAbs) and 14 monoclonal antibodies (mAbs) directed against hGH, hPRL, beta hFSH, beta hLH, beta hTSH and alpha-subunit were assessed by five different laboratories on normal and adenomatous pituitary tissues. This study aims at providing pathologists with a selected panel of antisera suitable for diagnosis, and appreciating the interest of the recently introduced mAbs. All the anti-hGH Abs proved to be specific (3 pAbs and 4 mAbs); three mAb out of four gave a few false-negative reactions. Three out of six polyclonal anti-hPRL showed cross-reactivity with hGH; anti-hPRL mAbs gave a strong staining with no false-negativity detected so far. MAbs proved to be more efficient for detecting glycoprotein hormones and alpha subunit than pAbs, which, in several cases, gave widespread cross-reactivity. This lack of specificity could explain the noticeable discrepancies reported so far in the appraisal of gonadotropic and somatoprolactinic adenomas. 0344-0338/9110187-0534$3.5010
© 1991 by Gustav Fischer Verlag, Stuttgart
Evaluation of 29 Monoclonal and Polyclonal Antibodies· 535
Introduction The accurate histological diagnosis of pituitary tumors as well as their classification and epidemiology rely mostly on immunocytochemistry. It is necessary to identify not only the various hormones but also their subunits synthetized by tumoral cells. Pathologists have at their disposal polyclonal and monoclonal antibodies which are said to be both specific and suitable for use on tissue sections. Nevertheless, some doubts have risen as to whether this is really the case. The pathologists of the "Club Fran
Evaluation of 29 Monoclonal and Polyclonal Antibodies Used in the Diagnosis of Pituitary Adenomas A Collaborative Study from Pathologists of the Club Fran\ais de I'Hypophyse F. Labat-Moleur Laboratoire de Pathologie Gel/ulaire, Hopital Albert Michal/on, Grenoble
J. Trouillas Laboratoire d'Histologie et d'Embryologie, Faculte de Medecine Alexis Garrel, Lyon
D. Seret-Begue Departement de Pathologie, Hopital Lariboisiere, Paris
M. Kujas Departement de Pathologie, Hopital La Pitie-$alp{ltriere, Paris
M.-B. Delisle Departement de Pathologie, Hopital Rangueil, Toulouse
C. Ronin Laboratoire de Biochimie, Faculte Nord de Medecine, Marseille, France
SUMMARY Fifteen polyclonal antibodies (pAbs) and 14 monoclonal antibodies (mAbs) directed against hGH, hPRL, beta hFSH, beta hLH, beta hTSH and alpha-subunit were assessed by five different laboratories on normal and adenomatous pituitary tissues. This study aims at providing pathologists with a selected panel of antisera suitable for diagnosis, and appreciating the interest of the recently introduced mAbs. All the anti-hGH Abs proved to be specific (3 pAbs and 4 mAbs); three mAb out of four gave a few false-negative reactions. Three out of six polyclonal anti-hPRL showed cross-reactivity with hGH; anti-hPRL mAbs gave a strong staining with no false-negativity detected so far. MAbs proved to be more efficient for detecting glycoprotein hormones and alpha subunit than pAbs, which, in several cases, gave widespread cross-reactivity. This lack of specificity could explain the noticeable discrepancies reported so far in the appraisal of gonadotropic and somatoprolactinic adenomas. 0344-0338/9110187-0534$3.5010
© 1991 by Gustav Fischer Verlag, Stuttgart
Evaluation of 29 Monoclonal and Polyclonal Antibodies· 535
Introduction The accurate histological diagnosis of pituitary tumors as well as their classification and epidemiology rely mostly on immunocytochemistry. It is necessary to identify not only the various hormones but also their subunits synthetized by tumoral cells. Pathologists have at their disposal polyclonal and monoclonal antibodies which are said to be both specific and suitable for use on tissue sections. Nevertheless, some doubts have risen as to whether this is really the case. The pathologists of the "Club Fran
