Veterinary Immunology and Immunopathology, 29 ( 1991 ) 105- i i 3
i05
Elsevier Science Publishers B.V., Amsterdam
Estimation of immunity in the developing calf: cellular and humorai responses to keyhole limpet haemocyanin J . M . P o l l o c k a, T . G . R o w a n b, J.B. D i x o n a'~, S . D . C a r t e r a'c a n d D . F . K e l l y a
aDepartments of Veterinary Pathology, bAnimai Husbandry and cVeterinary Clinical Sciences, University of LiverpooL P.O. Box 147, Liverpool L69 3BX. UK (Accepted 7 August 1990)
ABSTRACT Pollock, J.M., Rowan, T.G., Dixon, J.B., Carter, S.D. and Kelly, D.F., 1991. Estimation of immunity in the developing calf: cellular and humoral responses to keyhole limpet haemocyanin. Vet. hnmunoL hmnunopathoL, 29:105-113. The efficacy of keyhole limpet haemocyanin (KLH) as a test antigen was determined in calves. Humoral and cellular (in vivo and in vitro) responses were compared. Calves were immunized with KLH at either 3 weeks or up to 5 months of age and immune responses were subsequently tested. Class and subclass antibody responses were detected by ELISA, lymphocyte blastogenesis was measured using a whole blood culture technique (LTT) and skin sensitivity responses were measured as an increase in skin thickness following intradermal injection. In young calves, skin test responses were maximal at 24 h and were found to correlate with IgG 1 and IgG2 responses (P< 0.01 ), with IgA (P< 0.05) but not with IgM or LTT. Histological examination of skin swellings found a sequence of cellular events, with polymorphonuclear cells dominating until 48 h after intradermai injection, when mononuclear cells became involved. However, in ~,,~,A"~calves, skin test responses correlated not only with IgG 1 and IgA responses but also with lymphocyte transformation (P
i05
Elsevier Science Publishers B.V., Amsterdam
Estimation of immunity in the developing calf: cellular and humorai responses to keyhole limpet haemocyanin J . M . P o l l o c k a, T . G . R o w a n b, J.B. D i x o n a'~, S . D . C a r t e r a'c a n d D . F . K e l l y a
aDepartments of Veterinary Pathology, bAnimai Husbandry and cVeterinary Clinical Sciences, University of LiverpooL P.O. Box 147, Liverpool L69 3BX. UK (Accepted 7 August 1990)
ABSTRACT Pollock, J.M., Rowan, T.G., Dixon, J.B., Carter, S.D. and Kelly, D.F., 1991. Estimation of immunity in the developing calf: cellular and humoral responses to keyhole limpet haemocyanin. Vet. hnmunoL hmnunopathoL, 29:105-113. The efficacy of keyhole limpet haemocyanin (KLH) as a test antigen was determined in calves. Humoral and cellular (in vivo and in vitro) responses were compared. Calves were immunized with KLH at either 3 weeks or up to 5 months of age and immune responses were subsequently tested. Class and subclass antibody responses were detected by ELISA, lymphocyte blastogenesis was measured using a whole blood culture technique (LTT) and skin sensitivity responses were measured as an increase in skin thickness following intradermal injection. In young calves, skin test responses were maximal at 24 h and were found to correlate with IgG 1 and IgG2 responses (P< 0.01 ), with IgA (P< 0.05) but not with IgM or LTT. Histological examination of skin swellings found a sequence of cellular events, with polymorphonuclear cells dominating until 48 h after intradermai injection, when mononuclear cells became involved. However, in ~,,~,A"~calves, skin test responses correlated not only with IgG 1 and IgA responses but also with lymphocyte transformation (P
