DOI: 10.1038/ncomms9320
OPEN
Erratum: A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration Xuan Cao, Tomonori Kaneko, Jenny S. Li, An-Dong Liu, Courtney Voss & Shawn S.C. Li Nature Communications 6:7721 doi: 10.1038/ncomms8721 (2015); Published 13 Jul 2015; Updated 8 Sep 2015 The image in Fig. 1h of this Article was inadvertently duplicated from panel i. The correct version of the figure appears below.
b 5
10 30 kDa 130 55 130 55
WCL IB: TNS3
130
EGF min IP: p85 IB: TNS3 IP: p85 IB: PTEN IP: p85 IB: p85
c 0
5
10 30 kDa
Thr319 Thr321
PTEN
CAT
130 55 95
WCL IB: PTEN WCL IB: TNS3
Tyr321 Thr323 TNS3 -ABD
N
f
FLAG-DLC1
130
e
FLAG-DLC1
GFP-
PTEN TNS3-ABD
EGF – 30 min IP: GFP IB: FLAG
+
–
+
EGF – 30 min IP: GFP IB: p85 IP: GFP IB: GFP WCL BI: p85
kDa 130
IP: GFP IB: GFP
72
WCL BI: FLAg
130
PTEN TNS3-ABD
IP: p85 IB: GFP IP: p85 IB: p85
+
kDa
72 55 36 28
IP: GFP IB: GFP
95 72 95
72 55
IP: FLAG IB: GFP
36 28 130
72 55 36 28 72 55 36 28 95
IP: GFP IB: FLAG
130
IP: GFP IB: GFP
72 55 36 28
IP: FLAG IB: GFP IP: FLAG IB: FLAG
72 55 36 28 130
IP: GFP IB: p85 IP: GFP IB: GFP
IP: p85 IB: GFP IP: p85 IB: p85
EN PT -C EN AT -C 2
GFP-
kDa
EN
GFP-
PT
kDa 95
i
FLAG-DLC1 C tr l PT EN PT EN PT -CA EN T -C 2
TN
C tr l
IP: GFP IB: GFP
S3 AB -AB D D AB -N D -C 2
h
IP: GFP IB: p85
–
IP: FLAG IB: FLAG
g GFP-
+
kDa 130
IP: GFP IB: FLAG
C tr l
GFP-
C2
55
GFP-
d
C2
PT
EGF min 0 IP: DLC1 IB: TNS3 IP: DLC1 IB: PTEN IP: DLC1 IB: DLC1 WCL IB: PTEN
C trl TN S3 AB -AB D D AB N D -C 2
a
kDa 95 72 55 36 28 72 55 36 28 95
Figure 1 NATURE COMMUNICATIONS | 6:8320 | DOI: 10.1038/ncomms9320 | www.nature.com/naturecommunications
& 2015 Macmillan Publishers Limited. All rights reserved.
1
ERRATUM
NATURE COMMUNICATIONS | DOI: 10.1038/ncomms9320
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
2
NATURE COMMUNICATIONS | 6:8320 | DOI: 10.1038/ncomms9320 | www.nature.com/naturecommunications
& 2015 Macmillan Publishers Limited. All rights reserved.
OPEN
Erratum: A phosphorylation switch controls the spatiotemporal activation of Rho GTPases in directional cell migration Xuan Cao, Tomonori Kaneko, Jenny S. Li, An-Dong Liu, Courtney Voss & Shawn S.C. Li Nature Communications 6:7721 doi: 10.1038/ncomms8721 (2015); Published 13 Jul 2015; Updated 8 Sep 2015 The image in Fig. 1h of this Article was inadvertently duplicated from panel i. The correct version of the figure appears below.
b 5
10 30 kDa 130 55 130 55
WCL IB: TNS3
130
EGF min IP: p85 IB: TNS3 IP: p85 IB: PTEN IP: p85 IB: p85
c 0
5
10 30 kDa
Thr319 Thr321
PTEN
CAT
130 55 95
WCL IB: PTEN WCL IB: TNS3
Tyr321 Thr323 TNS3 -ABD
N
f
FLAG-DLC1
130
e
FLAG-DLC1
GFP-
PTEN TNS3-ABD
EGF – 30 min IP: GFP IB: FLAG
+
–
+
EGF – 30 min IP: GFP IB: p85 IP: GFP IB: GFP WCL BI: p85
kDa 130
IP: GFP IB: GFP
72
WCL BI: FLAg
130
PTEN TNS3-ABD
IP: p85 IB: GFP IP: p85 IB: p85
+
kDa
72 55 36 28
IP: GFP IB: GFP
95 72 95
72 55
IP: FLAG IB: GFP
36 28 130
72 55 36 28 72 55 36 28 95
IP: GFP IB: FLAG
130
IP: GFP IB: GFP
72 55 36 28
IP: FLAG IB: GFP IP: FLAG IB: FLAG
72 55 36 28 130
IP: GFP IB: p85 IP: GFP IB: GFP
IP: p85 IB: GFP IP: p85 IB: p85
EN PT -C EN AT -C 2
GFP-
kDa
EN
GFP-
PT
kDa 95
i
FLAG-DLC1 C tr l PT EN PT EN PT -CA EN T -C 2
TN
C tr l
IP: GFP IB: GFP
S3 AB -AB D D AB -N D -C 2
h
IP: GFP IB: p85
–
IP: FLAG IB: FLAG
g GFP-
+
kDa 130
IP: GFP IB: FLAG
C tr l
GFP-
C2
55
GFP-
d
C2
PT
EGF min 0 IP: DLC1 IB: TNS3 IP: DLC1 IB: PTEN IP: DLC1 IB: DLC1 WCL IB: PTEN
C trl TN S3 AB -AB D D AB N D -C 2
a
kDa 95 72 55 36 28 72 55 36 28 95
Figure 1 NATURE COMMUNICATIONS | 6:8320 | DOI: 10.1038/ncomms9320 | www.nature.com/naturecommunications
& 2015 Macmillan Publishers Limited. All rights reserved.
1
ERRATUM
NATURE COMMUNICATIONS | DOI: 10.1038/ncomms9320
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
2
NATURE COMMUNICATIONS | 6:8320 | DOI: 10.1038/ncomms9320 | www.nature.com/naturecommunications
& 2015 Macmillan Publishers Limited. All rights reserved.
