Life Sciences, Vol. 48, pp. 2457-2462 Printed in the U.S.A.

ENZYMATIC

Pergamon Press

ACTIVITY AND DISTRIBUTION OF P H O S P H O L I P A S E IN HUMAN CARTILAGE.

W. Pruzanski,

E. Bogoch,

E. Stefanski,

A2

M. Wloch and P. Vadas

I n f l a m m a t i o n Research Group, Departments of M e d i c i n e and Surgery, The W e l l e s l e y Hospital, University of Toronto, Ontario, Canada (Received in final form April 12, 1991) SUMMARY Extracellular phospholipase A2 (PLA2) with .proi n f l a m m a t o r y activity has recently been a l s c o v e r e a in synovial fluids in inflammatory arthritides. In the search for the sources of synovial fluid PLA2, human s y n o v i u m and articular cartilage were found to contain large q u a n t i t i e s of the enzyme. In rheumatoid arthritis (RA), PLA 2 activity in synovium, superficial and deep layers of articular cartilage was 20± 14 (SEM), 168± 62 and 533±176 nmol/min/mg protein respectively. Corresponding values in osteoarthritis (OA) were 49±11, 569±109 and 1709±243 nmol/min/mg protein, all s i g n i f i c a n t l y higher (p

Enzymatic activity and distribution of phospholipase A2 in human cartilage.

Extracellular phospholipase A2 (PLA2) with proinflammatory activity has recently been discovered in synovial fluids in inflammatory arthritides. In th...
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