Clin,. exp. Immunol. (1977) 27, 254-260.
Enhancement of in vitro immunoglobulin synthesis of human lymphocytes by lysosomal enzymes from polymorphonuclear leucocytes KUNIOMI YAMASAKI* & MORRIS ZIFF Rheumatic Diseases Unit, Department of Internal Medicine, University of Texas Health Science Center at Dallas, Dallas, Texas
(Received 3 August 1976)
SUMMARY
The effect of human peripheral blood polvmorphonuclear leucocv te (PMN) extracts and PMN granule lysates on in vitro immunoglobulin (Ig) synthesis by autologous peripheral blood mononuclear cells was studied. The mononuclear cells were cultured for 3 days with or without autologous plasma. Newly synthesized Ig in the culture supernatants was measured using '4C-labelled amino acids by an immune coprecipitation method. Upon addition of a PMN extract to plasma-free cultures Ig synthesis was stimulated, the mean stimulation index (SI) of cultures from thirteen individuals, including nine normals, three patients with rheumatoid arthritis and one with psoriatic arthritis being 1-8+0±2 in comparison with control cultures (P
Enhancement of in vitro immunoglobulin synthesis of human lymphocytes by lysosomal enzymes from polymorphonuclear leucocytes KUNIOMI YAMASAKI* & MORRIS ZIFF Rheumatic Diseases Unit, Department of Internal Medicine, University of Texas Health Science Center at Dallas, Dallas, Texas
(Received 3 August 1976)
SUMMARY
The effect of human peripheral blood polvmorphonuclear leucocv te (PMN) extracts and PMN granule lysates on in vitro immunoglobulin (Ig) synthesis by autologous peripheral blood mononuclear cells was studied. The mononuclear cells were cultured for 3 days with or without autologous plasma. Newly synthesized Ig in the culture supernatants was measured using '4C-labelled amino acids by an immune coprecipitation method. Upon addition of a PMN extract to plasma-free cultures Ig synthesis was stimulated, the mean stimulation index (SI) of cultures from thirteen individuals, including nine normals, three patients with rheumatoid arthritis and one with psoriatic arthritis being 1-8+0±2 in comparison with control cultures (P
![[Effects of anti-inflammatory agents on release of lysosomal enzymes from rabbit polymorphonuclear leucocytes induced by phospholipase C (author's transl)].](/assets/img/hold.png)
