Accepted Manuscript Effects of vitrification for GV and MII oocytes on subsequent centromere cohesion and chromosome aneuploidy in mice Jinmei Cheng , Baoyu Jia , Tianyu Wu , Guangbin Zhou , Yunpeng Hou , Xiangwei Fu , Shien Zhu PII:
S0093-691X(14)00231-3
DOI:
10.1016/j.theriogenology.2014.05.009
Reference:
THE 12801
To appear in:
Theriogenology
Received Date: 22 January 2014 Revised Date:
13 May 2014
Accepted Date: 13 May 2014
Please cite this article as: Cheng J, Jia B, Wu T, Zhou G, Hou Y, Fu X, Zhu S, Effects of vitrification for GV and MII oocytes on subsequent centromere cohesion and chromosome aneuploidy in mice, Theriogenology (2014), doi: 10.1016/j.theriogenology.2014.05.009. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Revisied
ACCEPTED MANUSCRIPT 1 2
Effects of vitrification for GV and MII oocytes on subsequent centromere cohesion and chromosome aneuploidy in mice
4
Jinmei Cheng a, Baoyu Jia a, Tianyu Wu a, Guangbin Zhou c, Yunpeng Hou b, Xiangwei Fu a d, Shien Zhu a d.
5
a
6
University, Beijing, P.R., China;
7
Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University
8
(Chengdu Campus), Wenjiang, P.R., China;
9
d Corresponding
RI PT
3
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural
author: Shien Zhu; E-mail address: [email protected];
10
Xiangwei Fu; E-mail address: [email protected]
11
Tel.:+86 10 62731767; fax: +86 10 62731767.
TE D
12 13
17 18 19 20
AC C
16
EP
14 15
SC
College of Biological Science, China Agricultural University, Beijing, P.R., China;
M AN U
b
21 22 23 24 25 1
c
ACCEPTED MANUSCRIPT Abstract: The present study examined the effect of vitrification on oocyte aneuploidy and centromere
27
cohesion. Firstly, germinal vesicle (GV) and in vitro matured oocytes (metaphase II, MII) were vitrified by
28
open-pulled straw (OPS) method. Secondly, thawed GV oocytes were matured in vitro to detect the
29
aneuploidy rate and the sister inter-kinetochore (iKT) distance (situ spreading and immunofluorescent
30
staining). The results showed that the sister iKT distance and the aneuploidy rate in eggs matured from
31
vitrified-thawed GV oocytes were higher than that of in vivo-matured, in vitro-matured and in
32
vitro-matured frozen oocytes (0.47 ± 0.03 μm vs. 0.33 ± 0.01 μm vs. 0.33 ± 0.02 μm vs. 0.34 ± 0.01 μm, P
S0093-691X(14)00231-3
DOI:
10.1016/j.theriogenology.2014.05.009
Reference:
THE 12801
To appear in:
Theriogenology
Received Date: 22 January 2014 Revised Date:
13 May 2014
Accepted Date: 13 May 2014
Please cite this article as: Cheng J, Jia B, Wu T, Zhou G, Hou Y, Fu X, Zhu S, Effects of vitrification for GV and MII oocytes on subsequent centromere cohesion and chromosome aneuploidy in mice, Theriogenology (2014), doi: 10.1016/j.theriogenology.2014.05.009. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Revisied
ACCEPTED MANUSCRIPT 1 2
Effects of vitrification for GV and MII oocytes on subsequent centromere cohesion and chromosome aneuploidy in mice
4
Jinmei Cheng a, Baoyu Jia a, Tianyu Wu a, Guangbin Zhou c, Yunpeng Hou b, Xiangwei Fu a d, Shien Zhu a d.
5
a
6
University, Beijing, P.R., China;
7
Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University
8
(Chengdu Campus), Wenjiang, P.R., China;
9
d Corresponding
RI PT
3
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural
author: Shien Zhu; E-mail address: [email protected];
10
Xiangwei Fu; E-mail address: [email protected]
11
Tel.:+86 10 62731767; fax: +86 10 62731767.
TE D
12 13
17 18 19 20
AC C
16
EP
14 15
SC
College of Biological Science, China Agricultural University, Beijing, P.R., China;
M AN U
b
21 22 23 24 25 1
c
ACCEPTED MANUSCRIPT Abstract: The present study examined the effect of vitrification on oocyte aneuploidy and centromere
27
cohesion. Firstly, germinal vesicle (GV) and in vitro matured oocytes (metaphase II, MII) were vitrified by
28
open-pulled straw (OPS) method. Secondly, thawed GV oocytes were matured in vitro to detect the
29
aneuploidy rate and the sister inter-kinetochore (iKT) distance (situ spreading and immunofluorescent
30
staining). The results showed that the sister iKT distance and the aneuploidy rate in eggs matured from
31
vitrified-thawed GV oocytes were higher than that of in vivo-matured, in vitro-matured and in
32
vitro-matured frozen oocytes (0.47 ± 0.03 μm vs. 0.33 ± 0.01 μm vs. 0.33 ± 0.02 μm vs. 0.34 ± 0.01 μm, P
