Int J Clin Exp Pathol 2015;8(6):6169-6180 www.ijcep.com /ISSN:1936-2625/IJCEP0009001
Original Article Effects of Polygonatum sibiricum polysaccharide on the osteogenic differentiation of bone mesenchymal stem cells in mice Shaohui Zong1, Gaofeng Zeng2, Bin Zou3, Keke Li3, Ye Fang3, Li Lu2, Deqiang Xiao2, Zhiyong Zhang2 Department of Osteopathia, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; College of Public Hygiene of Guangxi Medical University, Nanning, Guangxi, China; 3Graduate School of Guangxi Medical University, Nanning, Guangxi, China 1 2
Received April 11, 2015; Accepted May 27, 2015; Epub June 1, 2015; Published June 15, 2015 Abstract: Polygonatum sibiricum polysaccharide (PSP) is a traditional Chinese medicine and is widely used to treat many diseases for hundreds of years conventionally. This study was to access the effects of PSP on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in the mice. Cells collected from BALB/C mice in the bone marrow were isolated and cultured with osteogenic medium (OM) with different concentrations of PSP. The proliferation and morphological changes of BMSCs were observed using an inverted microscope. Flow cytometric analysis was used to identify the BMSCs. MTT test was performed to analyze the proliferation and viability of the cells. ELISA was used to determine the expression levels of alkaline phosphatase (ALP), osteocalcin (OC), N-terminal propeptide of type I procollagen (PINP) and bone morphogenetic protein-2 (BMP-2). Immunocytochemistry and western blot were respectively used to determine the expressions of bone sialoprotein (BSP) and SPARC/osteonectin (OSN). The growth curves of the proliferation and differentiation of the Control, OM, 17β-E2 and PSP groups were increased. Compared to the Control and OM groups, the expression levels of ALP, OC, PINP and BMP-2 were significantly increased in the PSP induced group (P
Original Article Effects of Polygonatum sibiricum polysaccharide on the osteogenic differentiation of bone mesenchymal stem cells in mice Shaohui Zong1, Gaofeng Zeng2, Bin Zou3, Keke Li3, Ye Fang3, Li Lu2, Deqiang Xiao2, Zhiyong Zhang2 Department of Osteopathia, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; College of Public Hygiene of Guangxi Medical University, Nanning, Guangxi, China; 3Graduate School of Guangxi Medical University, Nanning, Guangxi, China 1 2
Received April 11, 2015; Accepted May 27, 2015; Epub June 1, 2015; Published June 15, 2015 Abstract: Polygonatum sibiricum polysaccharide (PSP) is a traditional Chinese medicine and is widely used to treat many diseases for hundreds of years conventionally. This study was to access the effects of PSP on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in the mice. Cells collected from BALB/C mice in the bone marrow were isolated and cultured with osteogenic medium (OM) with different concentrations of PSP. The proliferation and morphological changes of BMSCs were observed using an inverted microscope. Flow cytometric analysis was used to identify the BMSCs. MTT test was performed to analyze the proliferation and viability of the cells. ELISA was used to determine the expression levels of alkaline phosphatase (ALP), osteocalcin (OC), N-terminal propeptide of type I procollagen (PINP) and bone morphogenetic protein-2 (BMP-2). Immunocytochemistry and western blot were respectively used to determine the expressions of bone sialoprotein (BSP) and SPARC/osteonectin (OSN). The growth curves of the proliferation and differentiation of the Control, OM, 17β-E2 and PSP groups were increased. Compared to the Control and OM groups, the expression levels of ALP, OC, PINP and BMP-2 were significantly increased in the PSP induced group (P
