Carcinogenesb vol.13 no.12 pp.22O5-221O, 1992
ACCELERATED PAPER
Effects of phenethyl isothiocyanate, a carcinogenesis inhibitor, on xenobiotic-metabolizing enzymes and nitrosamine metabolism in rats Zuyu Guo, Theresa J.Smith, Erjia Wang, Nakissa Sadrieh, Qiang Ma, Paul E.Thomas and Chung S.Yang1 Laboratory for Cancer Research, College of Pharmacy, Rutgers University, Piscataway, New Jersey 08855, USA 'To whom all correspondence should be addressed
•Abbreviations: PEITC, phenethyl isothiocyanate; PROD, pentoxyresorufin Odealkylase; EROD, ethoxyresorufin 0-dealkylase; NDMA, JV-nitrosodimethylamine; NDMAd, Af-nhrosodimethylamine demethylase; P450, cytochrome P-450; P450 reductase, NADPH:cytochrome P450 oxidoreductase; NNK, 4-induced DNA adduct formation and tumorigenicity in lung of F344 rats by dietary phenethyl isothiocyanate. Cancer Res., 49, 549—553. 5.Morse,S.A., Amin.S.G., Hecht.S.S. and Chung,F.-L. (1989) Effects of aromatic isothiocyanates on tumorigenicity, O6-methylguanine formation and metabolism of the tobacco-specific nitrosamine 4-(methylnitrosamino)-l(3-pyridyl}-l-butanone in A/J mouse lung. Cancer Res., 49, 2894-2897. 6. Morse.M.A., FJdind.K.I., Hecht.S.S., Jordan.K.G., Choi,C.-I., Desai.D.H., Amin.S.G. and Chung,F.-L. (1991) Structure—activity relationships for inhibition of 4-(methylnitrosamino)-l-(3-pyridyl)-l-butanone lung tumorigenesis by arylalkyl isothiocyanates in A/J mice. Cancer Res., 51, 1846-1850. 7. Wattenberg.L.W. (1977) Inhibition of carcinogenic effects of polycyclic hydrocarbons by benzyl isothiocyanate and related compounds. J. Nail Cancer lnst., 58, 395-398. 8. Hoffmann.D. and Hecht.S.S. (1985) Nicotine-derived A'-nitrosamines and tobacco-related cancer: current status and future directions. Cancer Res., 45, 935-944. 9. Magee.P.N., Montesano,P. and Preussmann.R. (1976)/V-nitroso compounds and related carcinogens. In C.E. Seale (ed.) Chemical Carcinogens. American Chemical Society Monograph 173, American Chemical Society, Washington, DC, pp. 491-625. 10. Yang.C.S., YooJ.-SH., Ishizaki,H. and Hong^.-Y. (1990) Cytochrome P450 DEI: roles in nitrosamine metabolism and mechanisms of regulation. Drug Metab. Rev., 22, 147-160. 11. Chung,F.-L., Wang.M. and Hecht.S.S. (1985) Effects of dietary indoles and isothiocyanates on Af-nitrosodimethylamine and 4-(methylnitrosamino)-l(3-pyridyl)-l-butanone a-hydroxylation and DNA methylation in rat liver. Carcinogenesis, 6, 539—543. 12. Ishizaki.H., BradyJ.F., Ning.S.M. and Yang.C.S. (1990) Effect of phenethyl isothiocyanate on microsomal A'-nitrosodimethylamine metabolism and other monooxygenase activities. Xenobiotica, 20, 255—264. 13. Lubet.R.A., Syi,J.-L., NelsonJ.O. and Nims.R.W. (1990) Induction of hepatic cytochrome P-450 mediated alkoxyresorufin 0-deallcylase activities in different species by prototype P450 inducers. Chem. -Biol. Interactions, 75, 325-339. 14. Watkins.P.B., Wrighton.S.A., Maurel,P., Schuetz.E.G., Mendez-Picon.G., Parker.G.A. and Guzelian.P.S. (1985) Identification of an inducible form of cytochrome P450 in human liver. Proc. Natt. Acad Sri. USA, 82, 6310-6314. 15. Yang.C.S., Strickhart.T.S. and Kicha.L.P. (1978) Analysis of the aryl hydrocarbon hydroxylase assay. Biochem. Pharmacol., 27, 2321-2326. 16. Tan,Y., Keefer,L.K. and Yang.C.S. (1987) Inhibition of microsomal A'nitrosodimethylamine demethylase by diethyl ether and other anesthetics. Biochem. Pharmacol., 36, 1973-1978. 17. ReinersJ.J., Cantu.A.R., Pavone.A., Smith,S.C., Gardner.C.R. and Laskin.D.L. (1990) Fluorescence assay for per-cell estimation of cytochrome P450-
ACCELERATED PAPER
Effects of phenethyl isothiocyanate, a carcinogenesis inhibitor, on xenobiotic-metabolizing enzymes and nitrosamine metabolism in rats Zuyu Guo, Theresa J.Smith, Erjia Wang, Nakissa Sadrieh, Qiang Ma, Paul E.Thomas and Chung S.Yang1 Laboratory for Cancer Research, College of Pharmacy, Rutgers University, Piscataway, New Jersey 08855, USA 'To whom all correspondence should be addressed
•Abbreviations: PEITC, phenethyl isothiocyanate; PROD, pentoxyresorufin Odealkylase; EROD, ethoxyresorufin 0-dealkylase; NDMA, JV-nitrosodimethylamine; NDMAd, Af-nhrosodimethylamine demethylase; P450, cytochrome P-450; P450 reductase, NADPH:cytochrome P450 oxidoreductase; NNK, 4-induced DNA adduct formation and tumorigenicity in lung of F344 rats by dietary phenethyl isothiocyanate. Cancer Res., 49, 549—553. 5.Morse,S.A., Amin.S.G., Hecht.S.S. and Chung,F.-L. (1989) Effects of aromatic isothiocyanates on tumorigenicity, O6-methylguanine formation and metabolism of the tobacco-specific nitrosamine 4-(methylnitrosamino)-l(3-pyridyl}-l-butanone in A/J mouse lung. Cancer Res., 49, 2894-2897. 6. Morse.M.A., FJdind.K.I., Hecht.S.S., Jordan.K.G., Choi,C.-I., Desai.D.H., Amin.S.G. and Chung,F.-L. (1991) Structure—activity relationships for inhibition of 4-(methylnitrosamino)-l-(3-pyridyl)-l-butanone lung tumorigenesis by arylalkyl isothiocyanates in A/J mice. Cancer Res., 51, 1846-1850. 7. Wattenberg.L.W. (1977) Inhibition of carcinogenic effects of polycyclic hydrocarbons by benzyl isothiocyanate and related compounds. J. Nail Cancer lnst., 58, 395-398. 8. Hoffmann.D. and Hecht.S.S. (1985) Nicotine-derived A'-nitrosamines and tobacco-related cancer: current status and future directions. Cancer Res., 45, 935-944. 9. Magee.P.N., Montesano,P. and Preussmann.R. (1976)/V-nitroso compounds and related carcinogens. In C.E. Seale (ed.) Chemical Carcinogens. American Chemical Society Monograph 173, American Chemical Society, Washington, DC, pp. 491-625. 10. Yang.C.S., YooJ.-SH., Ishizaki,H. and Hong^.-Y. (1990) Cytochrome P450 DEI: roles in nitrosamine metabolism and mechanisms of regulation. Drug Metab. Rev., 22, 147-160. 11. Chung,F.-L., Wang.M. and Hecht.S.S. (1985) Effects of dietary indoles and isothiocyanates on Af-nitrosodimethylamine and 4-(methylnitrosamino)-l(3-pyridyl)-l-butanone a-hydroxylation and DNA methylation in rat liver. Carcinogenesis, 6, 539—543. 12. Ishizaki.H., BradyJ.F., Ning.S.M. and Yang.C.S. (1990) Effect of phenethyl isothiocyanate on microsomal A'-nitrosodimethylamine metabolism and other monooxygenase activities. Xenobiotica, 20, 255—264. 13. Lubet.R.A., Syi,J.-L., NelsonJ.O. and Nims.R.W. (1990) Induction of hepatic cytochrome P-450 mediated alkoxyresorufin 0-deallcylase activities in different species by prototype P450 inducers. Chem. -Biol. Interactions, 75, 325-339. 14. Watkins.P.B., Wrighton.S.A., Maurel,P., Schuetz.E.G., Mendez-Picon.G., Parker.G.A. and Guzelian.P.S. (1985) Identification of an inducible form of cytochrome P450 in human liver. Proc. Natt. Acad Sri. USA, 82, 6310-6314. 15. Yang.C.S., Strickhart.T.S. and Kicha.L.P. (1978) Analysis of the aryl hydrocarbon hydroxylase assay. Biochem. Pharmacol., 27, 2321-2326. 16. Tan,Y., Keefer,L.K. and Yang.C.S. (1987) Inhibition of microsomal A'nitrosodimethylamine demethylase by diethyl ether and other anesthetics. Biochem. Pharmacol., 36, 1973-1978. 17. ReinersJ.J., Cantu.A.R., Pavone.A., Smith,S.C., Gardner.C.R. and Laskin.D.L. (1990) Fluorescence assay for per-cell estimation of cytochrome P450-
