Biol Trace Elem Res (2014) 157:138–146 DOI 10.1007/s12011-013-9876-4
Effects of Oligoelements Se, Zn, and Mn plus Lachesis Muta Venom in Experimental Scleroderma Ernesto J. V. Crescenti & Vanina A. Medina & Lorena A. Sambuco & Graciela A. Cremaschi & Ana M. Genaro & Graciela P. Cricco & Gabriela A. Martín & Eduardo Valli & Diego J. Martinel Lamas & Juan C. Perazzo & Elena S. Rivera & Rosa M. Bergoc
Received: 11 November 2013 / Accepted: 6 December 2013 / Published online: 22 December 2013 # Springer Science+Business Media New York 2013
Abstract Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM+O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and
BLM+O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956±59 μm, P
Effects of Oligoelements Se, Zn, and Mn plus Lachesis Muta Venom in Experimental Scleroderma Ernesto J. V. Crescenti & Vanina A. Medina & Lorena A. Sambuco & Graciela A. Cremaschi & Ana M. Genaro & Graciela P. Cricco & Gabriela A. Martín & Eduardo Valli & Diego J. Martinel Lamas & Juan C. Perazzo & Elena S. Rivera & Rosa M. Bergoc
Received: 11 November 2013 / Accepted: 6 December 2013 / Published online: 22 December 2013 # Springer Science+Business Media New York 2013
Abstract Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM+O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and
BLM+O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956±59 μm, P
