HHS Public Access Author manuscript Author Manuscript
Physiol Behav. Author manuscript; available in PMC 2017 October 01. Published in final edited form as: Physiol Behav. 2016 October 1; 164(Pt A): 383–389. doi:10.1016/j.physbeh.2016.06.006.
Effects of intrinsic aerobic capacity and ovariectomy on voluntary wheel running and nucleus accumbens dopamine receptor gene expression
Author Manuscript
Young-Min Park1, Jill A. Kanaley1, Jaume Padilla1,2,3, Terese Zidon1, Rebecca J. Welly1, Matthew J. Will4, Steven L. Britton5, Lauren G. Koch5, Gregory N. Ruegsegger6, Frank W. Booth6, John P. Thyfault7, and Victoria J. Vieira-Potter1 1Nutrition 2Child
and Exercise Physiology, University of Missouri, Columbia, MO, USA
Health, University of Missouri, Columbia, MO, USA
3Dalton
Cardiovascular Research Center, University of Missouri, Columbia, MO, USA
4Psychological 5Department 6Biomedical
Sciences, University of Missouri, Columbia, MO, USA
of Anesthesiology, University of Michigan Medical School, Ann Arbor, MI, USA
Sciences, University of Missouri, Columbia, MO, USA
7Department
of Molecular Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA
Author Manuscript
Abstract Rats selectively bred for high (HCR) and low (LCR) aerobic capacity show a stark divergence in wheel running behavior, which may be associated with dopamine (DA) system in the brain. HCR possess greater motivation for voluntary running along with greater brain DA activity compared to LCR. We recently demonstrated that HCR are not immune to ovariectomy (OVX)-associated reductions in spontaneous cage (i.e. locomotor) activity. Whether HCR and LCR rats differ in their OVX-mediated voluntary wheel running response is unknown. PURPOSE—To determine whether HCR are protected from OVX-associated reduction in voluntary wheel running.
Author Manuscript
METHODS—Forty female HCR and LCR rats (age ~27 weeks) had either SHM or OVX operations, and given access to a running wheel for 11 weeks. Weekly wheel running distance was monitored throughout the intervention. Nucleus accumbens (NAc) was assessed for mRNA expression of DA receptors at sacrifice.
Correspondence: Victoria J. Vieira-Potter, Assistant Professor, [email protected] 573 882 2027 (Ph), Department of Nutrition and Exercise Physiology, 310 Gwynn Hall, University of Missouri, Columbia, MO 65211. Disclosures: None. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Park et al.
Page 2
Author Manuscript
RESULTS—Compared to LCR, HCR ran greater distance and had greater ratio of excitatory/ inhibitory DA mRNA expression (both line main effects, P
Physiol Behav. Author manuscript; available in PMC 2017 October 01. Published in final edited form as: Physiol Behav. 2016 October 1; 164(Pt A): 383–389. doi:10.1016/j.physbeh.2016.06.006.
Effects of intrinsic aerobic capacity and ovariectomy on voluntary wheel running and nucleus accumbens dopamine receptor gene expression
Author Manuscript
Young-Min Park1, Jill A. Kanaley1, Jaume Padilla1,2,3, Terese Zidon1, Rebecca J. Welly1, Matthew J. Will4, Steven L. Britton5, Lauren G. Koch5, Gregory N. Ruegsegger6, Frank W. Booth6, John P. Thyfault7, and Victoria J. Vieira-Potter1 1Nutrition 2Child
and Exercise Physiology, University of Missouri, Columbia, MO, USA
Health, University of Missouri, Columbia, MO, USA
3Dalton
Cardiovascular Research Center, University of Missouri, Columbia, MO, USA
4Psychological 5Department 6Biomedical
Sciences, University of Missouri, Columbia, MO, USA
of Anesthesiology, University of Michigan Medical School, Ann Arbor, MI, USA
Sciences, University of Missouri, Columbia, MO, USA
7Department
of Molecular Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA
Author Manuscript
Abstract Rats selectively bred for high (HCR) and low (LCR) aerobic capacity show a stark divergence in wheel running behavior, which may be associated with dopamine (DA) system in the brain. HCR possess greater motivation for voluntary running along with greater brain DA activity compared to LCR. We recently demonstrated that HCR are not immune to ovariectomy (OVX)-associated reductions in spontaneous cage (i.e. locomotor) activity. Whether HCR and LCR rats differ in their OVX-mediated voluntary wheel running response is unknown. PURPOSE—To determine whether HCR are protected from OVX-associated reduction in voluntary wheel running.
Author Manuscript
METHODS—Forty female HCR and LCR rats (age ~27 weeks) had either SHM or OVX operations, and given access to a running wheel for 11 weeks. Weekly wheel running distance was monitored throughout the intervention. Nucleus accumbens (NAc) was assessed for mRNA expression of DA receptors at sacrifice.
Correspondence: Victoria J. Vieira-Potter, Assistant Professor, [email protected] 573 882 2027 (Ph), Department of Nutrition and Exercise Physiology, 310 Gwynn Hall, University of Missouri, Columbia, MO 65211. Disclosures: None. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Park et al.
Page 2
Author Manuscript
RESULTS—Compared to LCR, HCR ran greater distance and had greater ratio of excitatory/ inhibitory DA mRNA expression (both line main effects, P
