This article was downloaded by: [Monash University Library] On: 07 March 2015, At: 02:28 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
British Poultry Science Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/cbps20
Effects of growth hormone on tissue metabolism in broiler chicks a
a
a
a
C. G. Scanes , S. B. Telfer , A. F. Hackett , R. Nightingale & B. A. K. Sharifuddin
a
a
Department of Animal Physiology and Nutrition , University of Leeds , Leeds, LS2 9JT, England Published online: 08 Nov 2007.
To cite this article: C. G. Scanes , S. B. Telfer , A. F. Hackett , R. Nightingale & B. A. K. Sharifuddin (1975) Effects of growth hormone on tissue metabolism in broiler chicks, British Poultry Science, 16:4, 405-408, DOI: 10.1080/00071667508416204 To link to this article: http://dx.doi.org/10.1080/00071667508416204
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http://www.tandfonline.com/page/termsand-conditions
Br. Poult. Sci., 16: 405-408. 1975
Longman: printed in Great Britain
RESEARCH NOTE
EFFECTS OF GROWTH HORMONE ON TISSUE METABOLISM IN BROILER CHICKS C. G. SCANES, S. B. TELFER, A. F. HACKETT, R. NIGHTINGALE AND B. A. K. SHARIFUDDIN
Downloaded by [Monash University Library] at 02:28 07 March 2015
Department of Animal Physiology and Nutrition, University of Leeds, Leeds LS2 9 J T , England Received for publication 27th June 1974
1. The effect of bovine growth hormone (GH) on cartilage, liver, muscle, pancreas and spleen has been investigated. 2. Tissue RNA was elevated in the liver, muscle and spleen of the GH-treated chicks while uptake of radioactive phosphate into RNA was stimulated for both liver and spleen. 3. The GH-treated chicks had an increased cartilage protein content together with a higher rate of incorporation of 14C-methionine into pancreatic protein. INTRODUCTION
There is considerable evidence for the presence of growth hormone (GH) in the avian anterior pituitary gland (Nicoll and Licht, 1971). Indeed a preparation of duck GH has recently been obtained (Papkoff and Hayashida, 1972). However, little is known of the actions of GH on the metabolism of birds (Stockell Hartree and Cunningham, 1971). The present study, using broiler chicks, attempts to extend our limited knowledge on the actions of this hormone. Its influence on protein, RNA and DNA metabolism in various tissues has been examined. MATERIALS AND METHODS
Twenty-four unsexed i-d-old broiler chicks (Mansfield strain) were divided at random into two groups of equal mean body weight. They were fed ad libitum on a semi-synthetic diet (Morrisey and Wasserman, 1971). Each day the chicks were injected subcutaneously with either 10 /xg bovine GH (NIH-GH-B17) in 0-2 ml saline or the saline alone. The dose of GH was similar to that which had been found to increase organ weights in hypophysectomised pigeons (Bates et al., 1962). Sixteen hours prior to killing on the 10th d, all chicks were given an intraperitoneal injection of 0-2 ml of a solution containing 5 /nCi 14C-methionine (specific activity 62 mCi/mmol) and 25 /uCi 32P-phosphate/ml 0-9% saline. A further intraperitoneal injection of 0-2 ml of a solution of3H-thymidine (91 juCi/ml 0-9% saline, specific activity 17 Ci/mmol) was given 2 h before killing. 4°5
Downloaded by [Monash University Library] at 02:28 07 March 2015
32
Tissue Liver C GH G Muscle GH C Spleen GH Cartilage C GH Pancreas C
GH
Tissue wt (g) 3-90+ o-171 4-12 + 0-11 • ...
0-086 + 0-006 0-099 + 0-006 ...
0-45 + 0-02 0-47 + 0-02
fig RNA/g 226 + 21 630+19*** 215+18 288+18** 985136 1280+ 129* 598i8i 5"i43 i99±6 i6o±7***
P-phosphate incorporated into RNA (cpm/g) 133I16 216 + 29* 50 + 6 32 + 3** 279118 392134* 168 + 24 . «5417 NA NA 1
14
8
fig DNA/g 47o±25 465+15 138+11 156 + 22 2496+199 1965+101* 417157 409135 694133 522 + 40***
H-thymidine incorporated into DNA (cpm/g) 1211 +230 951 + 123 173132 245155 12731 + 1288 84741982 33001710 3650 + 666 293 + 62 349176
Mean values + SEM, 12 chicks per group. * P
British Poultry Science Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/cbps20
Effects of growth hormone on tissue metabolism in broiler chicks a
a
a
a
C. G. Scanes , S. B. Telfer , A. F. Hackett , R. Nightingale & B. A. K. Sharifuddin
a
a
Department of Animal Physiology and Nutrition , University of Leeds , Leeds, LS2 9JT, England Published online: 08 Nov 2007.
To cite this article: C. G. Scanes , S. B. Telfer , A. F. Hackett , R. Nightingale & B. A. K. Sharifuddin (1975) Effects of growth hormone on tissue metabolism in broiler chicks, British Poultry Science, 16:4, 405-408, DOI: 10.1080/00071667508416204 To link to this article: http://dx.doi.org/10.1080/00071667508416204
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http://www.tandfonline.com/page/termsand-conditions
Br. Poult. Sci., 16: 405-408. 1975
Longman: printed in Great Britain
RESEARCH NOTE
EFFECTS OF GROWTH HORMONE ON TISSUE METABOLISM IN BROILER CHICKS C. G. SCANES, S. B. TELFER, A. F. HACKETT, R. NIGHTINGALE AND B. A. K. SHARIFUDDIN
Downloaded by [Monash University Library] at 02:28 07 March 2015
Department of Animal Physiology and Nutrition, University of Leeds, Leeds LS2 9 J T , England Received for publication 27th June 1974
1. The effect of bovine growth hormone (GH) on cartilage, liver, muscle, pancreas and spleen has been investigated. 2. Tissue RNA was elevated in the liver, muscle and spleen of the GH-treated chicks while uptake of radioactive phosphate into RNA was stimulated for both liver and spleen. 3. The GH-treated chicks had an increased cartilage protein content together with a higher rate of incorporation of 14C-methionine into pancreatic protein. INTRODUCTION
There is considerable evidence for the presence of growth hormone (GH) in the avian anterior pituitary gland (Nicoll and Licht, 1971). Indeed a preparation of duck GH has recently been obtained (Papkoff and Hayashida, 1972). However, little is known of the actions of GH on the metabolism of birds (Stockell Hartree and Cunningham, 1971). The present study, using broiler chicks, attempts to extend our limited knowledge on the actions of this hormone. Its influence on protein, RNA and DNA metabolism in various tissues has been examined. MATERIALS AND METHODS
Twenty-four unsexed i-d-old broiler chicks (Mansfield strain) were divided at random into two groups of equal mean body weight. They were fed ad libitum on a semi-synthetic diet (Morrisey and Wasserman, 1971). Each day the chicks were injected subcutaneously with either 10 /xg bovine GH (NIH-GH-B17) in 0-2 ml saline or the saline alone. The dose of GH was similar to that which had been found to increase organ weights in hypophysectomised pigeons (Bates et al., 1962). Sixteen hours prior to killing on the 10th d, all chicks were given an intraperitoneal injection of 0-2 ml of a solution containing 5 /nCi 14C-methionine (specific activity 62 mCi/mmol) and 25 /uCi 32P-phosphate/ml 0-9% saline. A further intraperitoneal injection of 0-2 ml of a solution of3H-thymidine (91 juCi/ml 0-9% saline, specific activity 17 Ci/mmol) was given 2 h before killing. 4°5
Downloaded by [Monash University Library] at 02:28 07 March 2015
32
Tissue Liver C GH G Muscle GH C Spleen GH Cartilage C GH Pancreas C
GH
Tissue wt (g) 3-90+ o-171 4-12 + 0-11 • ...
0-086 + 0-006 0-099 + 0-006 ...
0-45 + 0-02 0-47 + 0-02
fig RNA/g 226 + 21 630+19*** 215+18 288+18** 985136 1280+ 129* 598i8i 5"i43 i99±6 i6o±7***
P-phosphate incorporated into RNA (cpm/g) 133I16 216 + 29* 50 + 6 32 + 3** 279118 392134* 168 + 24 . «5417 NA NA 1
14
8
fig DNA/g 47o±25 465+15 138+11 156 + 22 2496+199 1965+101* 417157 409135 694133 522 + 40***
H-thymidine incorporated into DNA (cpm/g) 1211 +230 951 + 123 173132 245155 12731 + 1288 84741982 33001710 3650 + 666 293 + 62 349176
Mean values + SEM, 12 chicks per group. * P
