Accepted Manuscript The effects of DASH diet on lipid profiles and biomarkers of oxidative stress in overweight and obese women with polycystic ovary syndrome: a randomised clinical trial Zatollah Asemi, Ph.D Mansooreh Samimi, M.D Zohreh Tabassi, M.D Hossein Shakeri, M.Sc Sima-Sadat Sabihi, B.Sc Ahmad Esmaillzadeh, Ph.D PII:
S0899-9007(14)00142-7
DOI:
10.1016/j.nut.2014.03.008
Reference:
NUT 9255
To appear in:
Nutrition
Received Date: 9 October 2013 Revised Date:
20 November 2013
Accepted Date: 9 March 2014
Please cite this article as: Asemi Z, Samimi M, Tabassi Z, Shakeri H, Sabihi S-S, Esmaillzadeh A, The effects of DASH diet on lipid profiles and biomarkers of oxidative stress in overweight and obese women with polycystic ovary syndrome: a randomised clinical trial, Nutrition (2014), doi: 10.1016/ j.nut.2014.03.008. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
The effects of DASH diet on lipid profiles and biomarkers of oxidative stress in
2
overweight and obese women with polycystic ovary syndrome: a randomised
3
clinical trial
4
Zatollah Asemi Ph.D. a, Mansooreh Samimi M.D. b, Zohreh Tabassi M.D. b, Hossein Shakeri
5
M.Sc. a, Sima-Sadat Sabihi B.Sc. a, Ahmad Esmaillzadeh Ph.D. c,d,* a
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of
SC
6
RI PT
1
Medical Sciences, Kashan, I.R. Iran
7 b
Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical
M AN U
8
Sciences, Kashan, I.R. Iran
9 c
10 11
d
Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
12
Running title: DASH diet for PCOS
14 15
Z.A. conducted the study, carried out the statistical analyses, wrote the manuscript and
16
contributed in the interpretation of the findings. M.S,Z.T, H.Sh and S-S. S contributed in data
17
collection and assisted in writing the manuscript. A.E. contributed in conception and design and
18
also advised on statistical analyses and assisted in interpretation of the findings. None of the
19
authors had any personal or financial conflict of interest. All authors approved the final version
20
for submission Clinical trial registration number:www.irct.ir:IRCT201304235623N6.
21
* Corresponding author. Tel.: 98-311-7922720; fax: 98-311-6682509.
22
E-mail address: [email protected] (A. Esmaillzadeh).
AC C
EP
TE D
13
23
1
ACCEPTED MANUSCRIPT
Abstract
25
Objective: This study was designed to assess the effects of the Dietary Approaches to Stop
26
Hypertension (DASH) diet on lipid profiles and biomarkers of oxidative stress in overweight and
27
obese women with polycystic ovary syndrome (PCOS).
28
Methods: This randomised controlled clinical trial was done among 48women diagnosed with
29
PCOS. Subjects were randomly assigned to consume either the control (n=24) or the DASH
30
eating pattern (n=24) for 8 weeks. Both diets were designed to be calorie-restricted. The DASH
31
and control diets were consisted of 52% carbohydrates, 18% proteins, 30% total fats. The DASH
32
diet was designed to be rich in fruits, vegetables, whole grains, and low-fat dairy products and
33
low in saturated fats, cholesterol and refined grains. Fasting blood samples were taken at baseline
34
and after 8-wk intervention to measure lipid profiles and biomarkers of oxidative stress including
35
plasma total antioxidant capacity (TAC) and total glutathione (GSH).
36
Results: Adherence to the DASH diet, compared to the control diet, resulted in a significant
37
decrease in weight (-4.4 vs. -1.5 kg; P
S0899-9007(14)00142-7
DOI:
10.1016/j.nut.2014.03.008
Reference:
NUT 9255
To appear in:
Nutrition
Received Date: 9 October 2013 Revised Date:
20 November 2013
Accepted Date: 9 March 2014
Please cite this article as: Asemi Z, Samimi M, Tabassi Z, Shakeri H, Sabihi S-S, Esmaillzadeh A, The effects of DASH diet on lipid profiles and biomarkers of oxidative stress in overweight and obese women with polycystic ovary syndrome: a randomised clinical trial, Nutrition (2014), doi: 10.1016/ j.nut.2014.03.008. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
The effects of DASH diet on lipid profiles and biomarkers of oxidative stress in
2
overweight and obese women with polycystic ovary syndrome: a randomised
3
clinical trial
4
Zatollah Asemi Ph.D. a, Mansooreh Samimi M.D. b, Zohreh Tabassi M.D. b, Hossein Shakeri
5
M.Sc. a, Sima-Sadat Sabihi B.Sc. a, Ahmad Esmaillzadeh Ph.D. c,d,* a
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of
SC
6
RI PT
1
Medical Sciences, Kashan, I.R. Iran
7 b
Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical
M AN U
8
Sciences, Kashan, I.R. Iran
9 c
10 11
d
Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
12
Running title: DASH diet for PCOS
14 15
Z.A. conducted the study, carried out the statistical analyses, wrote the manuscript and
16
contributed in the interpretation of the findings. M.S,Z.T, H.Sh and S-S. S contributed in data
17
collection and assisted in writing the manuscript. A.E. contributed in conception and design and
18
also advised on statistical analyses and assisted in interpretation of the findings. None of the
19
authors had any personal or financial conflict of interest. All authors approved the final version
20
for submission Clinical trial registration number:www.irct.ir:IRCT201304235623N6.
21
* Corresponding author. Tel.: 98-311-7922720; fax: 98-311-6682509.
22
E-mail address: [email protected] (A. Esmaillzadeh).
AC C
EP
TE D
13
23
1
ACCEPTED MANUSCRIPT
Abstract
25
Objective: This study was designed to assess the effects of the Dietary Approaches to Stop
26
Hypertension (DASH) diet on lipid profiles and biomarkers of oxidative stress in overweight and
27
obese women with polycystic ovary syndrome (PCOS).
28
Methods: This randomised controlled clinical trial was done among 48women diagnosed with
29
PCOS. Subjects were randomly assigned to consume either the control (n=24) or the DASH
30
eating pattern (n=24) for 8 weeks. Both diets were designed to be calorie-restricted. The DASH
31
and control diets were consisted of 52% carbohydrates, 18% proteins, 30% total fats. The DASH
32
diet was designed to be rich in fruits, vegetables, whole grains, and low-fat dairy products and
33
low in saturated fats, cholesterol and refined grains. Fasting blood samples were taken at baseline
34
and after 8-wk intervention to measure lipid profiles and biomarkers of oxidative stress including
35
plasma total antioxidant capacity (TAC) and total glutathione (GSH).
36
Results: Adherence to the DASH diet, compared to the control diet, resulted in a significant
37
decrease in weight (-4.4 vs. -1.5 kg; P
