1726 Open Access Asian Australas. J. Anim. Sci. Vol. 27, No. 12 : 1726-1735 December 2014 http://dx.doi.org/10.5713/ajas.2014.14292
www.ajas.info pISSN 1011-2367 eISSN 1976-5517
Effects of Acarbose Addition on Ruminal Bacterial Microbiota, Lipopolysaccharide Levels and Fermentation Characteristics In vitro Yu-yang Yin, Yu-jie Liu, Wei-yun Zhu, and Sheng-yong Mao* College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China ABSTRACT: This study investigated the effects of acarbose addition on changes in ruminal fermentation characteristics and the composition of the ruminal bacterial community in vitro using batch cultures. Rumen fluid was collected from the rumens of three cannulated Holstein cattle fed forage ad libitum that was supplemented with 6 kg of concentrate. The batch cultures consisted of 8 mL of strained rumen fluid in 40 mL of an anaerobic buffer containing 0.49 g of corn grain, 0.21 g of soybean meal, 0.15 g of alfalfa and 0.15g of Leymus chinensis. Acarbose was added to incubation bottles to achieve final concentrations of 0.1, 0.2, and 0.4 mg/mL. After incubation for 24 h, the addition of acarbose linearly decreased (p
www.ajas.info pISSN 1011-2367 eISSN 1976-5517
Effects of Acarbose Addition on Ruminal Bacterial Microbiota, Lipopolysaccharide Levels and Fermentation Characteristics In vitro Yu-yang Yin, Yu-jie Liu, Wei-yun Zhu, and Sheng-yong Mao* College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China ABSTRACT: This study investigated the effects of acarbose addition on changes in ruminal fermentation characteristics and the composition of the ruminal bacterial community in vitro using batch cultures. Rumen fluid was collected from the rumens of three cannulated Holstein cattle fed forage ad libitum that was supplemented with 6 kg of concentrate. The batch cultures consisted of 8 mL of strained rumen fluid in 40 mL of an anaerobic buffer containing 0.49 g of corn grain, 0.21 g of soybean meal, 0.15 g of alfalfa and 0.15g of Leymus chinensis. Acarbose was added to incubation bottles to achieve final concentrations of 0.1, 0.2, and 0.4 mg/mL. After incubation for 24 h, the addition of acarbose linearly decreased (p
