Effect of 530nm monochromatic light on M俟ller cells
窑Basic Research窑
Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor - 茁1 expression in M俟ller cells Department of Ophthalmology, the First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China Correspondence to: Xiao-Feng Zhang. Department of Ophthalmology, the First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou 215000, Jiangsu Province, China. [email protected] Received:2014-09-29 Accepted:2015-01-26
DOI:10.3980/j.issn.2222-3959.2015.05.09
Abstract
M
· AIM:
To expose rat retinal M俟ller cells to 530 nm
monochromatic light and investigate the influence of
varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-茁1 (TGF-茁1) expression.
· METHODS:
Three groups of rat retinal M俟ller cells
cultured under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF -茁1 mRNA expression, protein levels and fluorescence intensity of the M俟ller cells were analyzed.
· RESULTS:
The bFGF mRNA expression and protein
levels were significantly upregulated in M俟ller cells in all three experimental groups compared with the control group (
窑Basic Research窑
Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor - 茁1 expression in M俟ller cells Department of Ophthalmology, the First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China Correspondence to: Xiao-Feng Zhang. Department of Ophthalmology, the First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou 215000, Jiangsu Province, China. [email protected] Received:2014-09-29 Accepted:2015-01-26
DOI:10.3980/j.issn.2222-3959.2015.05.09
Abstract
M
· AIM:
To expose rat retinal M俟ller cells to 530 nm
monochromatic light and investigate the influence of
varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-茁1 (TGF-茁1) expression.
· METHODS:
Three groups of rat retinal M俟ller cells
cultured under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF -茁1 mRNA expression, protein levels and fluorescence intensity of the M俟ller cells were analyzed.
· RESULTS:
The bFGF mRNA expression and protein
levels were significantly upregulated in M俟ller cells in all three experimental groups compared with the control group (
