EXPERIMENTAL AND THERAPEUTIC MEDICINE 7: 565-572, 2014
Effects and mechanisms of alveolar type II epithelial cell apoptosis in severe pancreatitis-induced acute lung injury GELIANG LIU1, JINGWEN ZHANG1, HAILONG CHEN1, CHAO WANG1, YANG QIU2, YUEJIAN LIU2, JIAJIA WAN2 and HUISHU GUO2 1
2
Department of General Surgery, First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011; Central Laboratory, Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning 116044, P.R. China Received July 24, 2013; Accepted November 28, 2013 DOI: 10.3892/etm.2013.1453
Abstract. This study aimed to examine the role of alveolar type II epithelial cell (AEC II) apoptosis in severe pancreatitis‑induced acute lung injury (ALI) and the intervening role of Qingyi decoction (QYT). An SAP model was established in male Sprague‑Dawley rats. Immunohistochemical analysis was conducted to observe the pathological changes in the pancreas and lung tissue. AEC II apoptosis was detected by flow cytometry and the free Ca 2+ concentration in AECs II was determined by laser scanning confocal microscopy. A radioimmunoassay was performed to determine serum TNF-α content. Quantitative polymerase chain reaction (qPCR) and immunohistochemical analysis were performed to detect the mRNA and protein expression levels of Bax and caspase-8 in the lung tissue. Hematoxylin and eosin staining of lung tissue sections in the severe acute pancreatitis (SAP) group showed pathological changes from control tissue, consistent with acute lung injury (ALI). Flow cytometry showed that the level of AEC II apoptosis in the SAP group was significantly increased compared with that in the control group (P
Effects and mechanisms of alveolar type II epithelial cell apoptosis in severe pancreatitis-induced acute lung injury GELIANG LIU1, JINGWEN ZHANG1, HAILONG CHEN1, CHAO WANG1, YANG QIU2, YUEJIAN LIU2, JIAJIA WAN2 and HUISHU GUO2 1
2
Department of General Surgery, First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011; Central Laboratory, Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning 116044, P.R. China Received July 24, 2013; Accepted November 28, 2013 DOI: 10.3892/etm.2013.1453
Abstract. This study aimed to examine the role of alveolar type II epithelial cell (AEC II) apoptosis in severe pancreatitis‑induced acute lung injury (ALI) and the intervening role of Qingyi decoction (QYT). An SAP model was established in male Sprague‑Dawley rats. Immunohistochemical analysis was conducted to observe the pathological changes in the pancreas and lung tissue. AEC II apoptosis was detected by flow cytometry and the free Ca 2+ concentration in AECs II was determined by laser scanning confocal microscopy. A radioimmunoassay was performed to determine serum TNF-α content. Quantitative polymerase chain reaction (qPCR) and immunohistochemical analysis were performed to detect the mRNA and protein expression levels of Bax and caspase-8 in the lung tissue. Hematoxylin and eosin staining of lung tissue sections in the severe acute pancreatitis (SAP) group showed pathological changes from control tissue, consistent with acute lung injury (ALI). Flow cytometry showed that the level of AEC II apoptosis in the SAP group was significantly increased compared with that in the control group (P
