www.scielo.br/jaos
http://dx.doi.org/10.1590/1678-775720140304
Effectiveness of plant-derived proanthocyanidins on demineralization on enamel and dentin under artificial cariogenic challenge Ana Paula Pereira da SILVA1, Rafael Simões GONÇALVES2, Ana Flávia Sanches BORGES2, Ana Karina BEDRANRUSSO3, Mirela Sanae SHINOHARA1 1- Department of Restorative Dentistry, Araçatuba Dental School, UNESP – Univ. Estadual Paulista, Araçatuba, SP, Brazil. 2- Department of Dentistry, Endodontic and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. 3- Department of Restorative Dentistry, College of Dentistry, University of Illinois, Chicago, IL, USA. Corresponding author: Prof. Dra. Mirela Sanae Shinohara - Programa de Pós-Graduação em Ciência Odontológica - Área de Concentração: Biomateriais - Faculdade de Odontologia de Araçatuba, UNESP - Univ. Estadual Paulista - R. José Bonifácio, 1193 - Araçatuba - SP - Brazil - 16015-050 - Fax: (+55)18 3636-3349 - E-mail: [email protected] Submitted: August 14, 2014 - Modification: October 22, 2014 - Accepted: January 22, 2015
ABSTRACT
D
ental caries is considered a disease of high prevalence and a constant problem in public health. Proanthocyanidins (PAs) are substances that have been the target of recent studies aiming to control or treat caries. Objective: The aim of this in vitro study was to evaluate the efficacy of a treatment with grape seed extract, under cariogenic challenge, to minimize or even prevent the onset of caries in the enamel and dentin. Material and Methods: Blocks of enamel and dentin (6.0x6.0 mm) were obtained from bovine central incisors, polished, and selected by analysis of surface microhardness (SH). The blocks were randomly divided into 3 groups (n=15), according to the following treatments: GC (control), GSE (grape seed extract), GF (fluoride – 1,000 ppm). The blocks were subjected to 6 daily pH cycles for 8 days. Within the daily cycling, the specimens were stored in buffered solution. The blocks were then analyzed for perpendicular and surface hardness and polarized light microscopy. Results: The means were subjected to statistical analysis using the ANOVA and Fisher’s PLSD tests (p
http://dx.doi.org/10.1590/1678-775720140304
Effectiveness of plant-derived proanthocyanidins on demineralization on enamel and dentin under artificial cariogenic challenge Ana Paula Pereira da SILVA1, Rafael Simões GONÇALVES2, Ana Flávia Sanches BORGES2, Ana Karina BEDRANRUSSO3, Mirela Sanae SHINOHARA1 1- Department of Restorative Dentistry, Araçatuba Dental School, UNESP – Univ. Estadual Paulista, Araçatuba, SP, Brazil. 2- Department of Dentistry, Endodontic and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. 3- Department of Restorative Dentistry, College of Dentistry, University of Illinois, Chicago, IL, USA. Corresponding author: Prof. Dra. Mirela Sanae Shinohara - Programa de Pós-Graduação em Ciência Odontológica - Área de Concentração: Biomateriais - Faculdade de Odontologia de Araçatuba, UNESP - Univ. Estadual Paulista - R. José Bonifácio, 1193 - Araçatuba - SP - Brazil - 16015-050 - Fax: (+55)18 3636-3349 - E-mail: [email protected] Submitted: August 14, 2014 - Modification: October 22, 2014 - Accepted: January 22, 2015
ABSTRACT
D
ental caries is considered a disease of high prevalence and a constant problem in public health. Proanthocyanidins (PAs) are substances that have been the target of recent studies aiming to control or treat caries. Objective: The aim of this in vitro study was to evaluate the efficacy of a treatment with grape seed extract, under cariogenic challenge, to minimize or even prevent the onset of caries in the enamel and dentin. Material and Methods: Blocks of enamel and dentin (6.0x6.0 mm) were obtained from bovine central incisors, polished, and selected by analysis of surface microhardness (SH). The blocks were randomly divided into 3 groups (n=15), according to the following treatments: GC (control), GSE (grape seed extract), GF (fluoride – 1,000 ppm). The blocks were subjected to 6 daily pH cycles for 8 days. Within the daily cycling, the specimens were stored in buffered solution. The blocks were then analyzed for perpendicular and surface hardness and polarized light microscopy. Results: The means were subjected to statistical analysis using the ANOVA and Fisher’s PLSD tests (p
