Effect of zinc supplementation on neuronal precursor proliferation in the rat hippocampus after traumatic brain injury Elise C. Cope, Deborah R. Morris, Shannon D. Gower-Winter, Naomi C. Brownstein, Cathy W. Levenson PII: DOI: Reference:
S0014-4886(16)30038-3 doi: 10.1016/j.expneurol.2016.02.017 YEXNR 12223
To appear in:
Experimental Neurology
Received date: Revised date: Accepted date:
20 November 2015 16 February 2016 18 February 2016
Please cite this article as: Cope, Elise C., Morris, Deborah R., Gower-Winter, Shannon D., Brownstein, Naomi C., Levenson, Cathy W., Effect of zinc supplementation on neuronal precursor proliferation in the rat hippocampus after traumatic brain injury, Experimental Neurology (2016), doi: 10.1016/j.expneurol.2016.02.017
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Effect of zinc supplementation on neuronal precursor proliferation in the rat hippocampus after traumatic brain injury.
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Elise C. Cope*1,5, Deborah R. Morris*1, Shannon D. Gower-Winter1, Naomi C. Brownstein3,4, and Cathy W. Levenson1,2 Department of Biomedical Sciences, 2Program in Neuroscience, 3Department of Behavioral Sciences and Social Medicine, Florida State University College of Medicine, Tallahassee, FL. 4Department of Statistics, Florida State University, Tallahassee, FL
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AC CE P
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Corresponding Author: Cathy W. Levenson, PhD Florida State University College of Medicine Tallahassee, FL 32306-4300
MA
Current Address: Department of Psychology Princeton Neuroscience Institute Peretsman-Scully Hall Princeton, NJ 08544
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*The first two authors listed contributed equally to this manuscript and should be considered first authors.
Phone: 850-644-4122 FAX: 850-644-5781 Email: [email protected]
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ACCEPTED MANUSCRIPT Abstract There is great deal of debate about the possible role of adult-born hippocampal cells in
PT
the prevention of depression and related mood disorders. We first showed that zinc
RI
supplementation prevents the development of the depression-like behavior anhedonia associated with an animal model of traumatic brain injury (TBI). This work then
SC
examined the effect of zinc supplementation on the proliferation of new cells in the
NU
hippocampus that have the potential to participate in neurogenesis. Rats were fed a zinc adequate (ZA, 30 ppm) or zinc supplemented (ZS, 180 ppm) diet for 4 wk followed
MA
by TBI using controlled cortical impact. Stereological counts of EdU-positive cells showed that TBI doubled the density of proliferating cells 24 h post-injury (p
S0014-4886(16)30038-3 doi: 10.1016/j.expneurol.2016.02.017 YEXNR 12223
To appear in:
Experimental Neurology
Received date: Revised date: Accepted date:
20 November 2015 16 February 2016 18 February 2016
Please cite this article as: Cope, Elise C., Morris, Deborah R., Gower-Winter, Shannon D., Brownstein, Naomi C., Levenson, Cathy W., Effect of zinc supplementation on neuronal precursor proliferation in the rat hippocampus after traumatic brain injury, Experimental Neurology (2016), doi: 10.1016/j.expneurol.2016.02.017
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Effect of zinc supplementation on neuronal precursor proliferation in the rat hippocampus after traumatic brain injury.
PT
Elise C. Cope*1,5, Deborah R. Morris*1, Shannon D. Gower-Winter1, Naomi C. Brownstein3,4, and Cathy W. Levenson1,2 Department of Biomedical Sciences, 2Program in Neuroscience, 3Department of Behavioral Sciences and Social Medicine, Florida State University College of Medicine, Tallahassee, FL. 4Department of Statistics, Florida State University, Tallahassee, FL
SC
RI
1
5
AC CE P
TE
D
Corresponding Author: Cathy W. Levenson, PhD Florida State University College of Medicine Tallahassee, FL 32306-4300
MA
Current Address: Department of Psychology Princeton Neuroscience Institute Peretsman-Scully Hall Princeton, NJ 08544
NU
*The first two authors listed contributed equally to this manuscript and should be considered first authors.
Phone: 850-644-4122 FAX: 850-644-5781 Email: [email protected]
1
ACCEPTED MANUSCRIPT Abstract There is great deal of debate about the possible role of adult-born hippocampal cells in
PT
the prevention of depression and related mood disorders. We first showed that zinc
RI
supplementation prevents the development of the depression-like behavior anhedonia associated with an animal model of traumatic brain injury (TBI). This work then
SC
examined the effect of zinc supplementation on the proliferation of new cells in the
NU
hippocampus that have the potential to participate in neurogenesis. Rats were fed a zinc adequate (ZA, 30 ppm) or zinc supplemented (ZS, 180 ppm) diet for 4 wk followed
MA
by TBI using controlled cortical impact. Stereological counts of EdU-positive cells showed that TBI doubled the density of proliferating cells 24 h post-injury (p
