CLINICAL RESEARCH e-ISSN 1643-3750 © Med Sci Monit, 2017; 23: 4175-4181 DOI: 10.12659/MSM.902237
Effect of Probiotic Lactobacilli on the Growth of Streptococcus Mutans and Multispecies Biofilms Isolated from Children with Active Caries
Received: 2016.11.15 Accepted: 2017.02.13 Published: 2017.08.30
Authors’ ABCE Contribution: Xiaolong Lin Study Design A AB Xi Chen Data Collection B CD Yan Tu Statistical Analysis C Data Interpretation AC D Sa Wang Manuscript Preparation E EG Hui Chen Literature Search F Funds Collection G
Department of Conservative Dentistry and Periodontics, Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China
Corresponding Author: Source of support:
Hui Chen, e-mail: [email protected] This study was supported by grants from the National Natural Science Foundation of China (Grant No. 81371142), Grants for Medical Technology of Zhejiang Province (Grant No. 2012ZDA0320), the Provincial and Ministerial Project of Zhejiang Province (Grant No. 2015120726 ) and the Education Department of Zhejiang Province (Grant No. 2012C34011)
Background:
Material/Methods:
Results:
Conclusions:
The purpose of this investigation was to evaluate the effect of probiotic lactobacilli on Streptococcus mutans (MS) and multispecies biofilms isolated from children with severe caries. Twenty children with active caries (DMFS ³6) were selected as the experimental group and Streptococcus mutans (MS) were isolated from their saliva. After identification the MS strains were mixed with lactobacilli at 37°C, following which viable MS colonies were counted. At the same time dental plaques from the children were mixed with lactobacilli in vitro to form biofilms, and the population of nine common strains in the biofilms was enumerated after 24 hours of growth. Lactobacillus casei Shirota, L. casei LC01, L. plantarum ST-III and L. paracasei LPC37 all had strong inhibitory effects on the majority the MS isolated from children with active caries, with the inhibition rate reaching approximately 70–90% (p
Effect of Probiotic Lactobacilli on the Growth of Streptococcus Mutans and Multispecies Biofilms Isolated from Children with Active Caries
Received: 2016.11.15 Accepted: 2017.02.13 Published: 2017.08.30
Authors’ ABCE Contribution: Xiaolong Lin Study Design A AB Xi Chen Data Collection B CD Yan Tu Statistical Analysis C Data Interpretation AC D Sa Wang Manuscript Preparation E EG Hui Chen Literature Search F Funds Collection G
Department of Conservative Dentistry and Periodontics, Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China
Corresponding Author: Source of support:
Hui Chen, e-mail: [email protected] This study was supported by grants from the National Natural Science Foundation of China (Grant No. 81371142), Grants for Medical Technology of Zhejiang Province (Grant No. 2012ZDA0320), the Provincial and Ministerial Project of Zhejiang Province (Grant No. 2015120726 ) and the Education Department of Zhejiang Province (Grant No. 2012C34011)
Background:
Material/Methods:
Results:
Conclusions:
The purpose of this investigation was to evaluate the effect of probiotic lactobacilli on Streptococcus mutans (MS) and multispecies biofilms isolated from children with severe caries. Twenty children with active caries (DMFS ³6) were selected as the experimental group and Streptococcus mutans (MS) were isolated from their saliva. After identification the MS strains were mixed with lactobacilli at 37°C, following which viable MS colonies were counted. At the same time dental plaques from the children were mixed with lactobacilli in vitro to form biofilms, and the population of nine common strains in the biofilms was enumerated after 24 hours of growth. Lactobacillus casei Shirota, L. casei LC01, L. plantarum ST-III and L. paracasei LPC37 all had strong inhibitory effects on the majority the MS isolated from children with active caries, with the inhibition rate reaching approximately 70–90% (p
