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Asian-Aust. J. Anim. Sci. Vol. 26, No. 2 : 178-188 February 2013 http://dx.doi.org/10.5713/ajas.2012.12406

www.ajas.info pISSN 1011-2367 eISSN 1976-5517

Effect of Glycosaminoglycans on In vitro Fertilizing Ability and In vitro Developmental Potential of Bovine Embryos Eun Young Kima, Eun Hyung Noha,1, Eun Ji Noh1, Min Jee Park1, Hyo Young Park, Dong Sun Lee1, Key Zung Riu1 and Se Pill Park1,* Mirae Biotech/Jeju National University Stem Cell Research Center, Seoul 143-193, Korea ABSTRACT: The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 g/ml of one of four GAGs (Chondroitin sulfate, CS; Dermatan sulfate, DS; Hyaluronic acid, HA; Heparin, HP) for 5 h, the total motility (TM), straight-line velocity (VSL), and curvilinear velocity (VCL) were higher in the HP- or HA-treated sperm, relative to control and CS- or DS-treated sperm. HP and HA treatments increased the levels of capacitated and acrosome-reacted sperm over time, compared to other treatment groups (p

Effect of Glycosaminoglycans on In vitro Fertilizing Ability and In vitro Developmental Potential of Bovine Embryos.

The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 μg/ml of one o...
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