9 1987 by The Humana Press Inc. All rights of any nature, whatsoever, reserved. 0163-4984/87/1200-0429502.00
Effect of Diabetes on Trace-Element Concentration of Blood M. A. CHAUDHRI, 1 M. KOULMANDA, 2 M. T. HAUKKA, 3 AND N. NOORBAKHS j
IDepartment of Medical Physics, Austin Hospital, IVlelboume, and University of h'lelboume, Australia; 2Walter and Eliza Hall Institute of Medical Research, h'lelboume, Australia; and 3Department of Geology, University of Melbourne,/Vleiboume, Australia.
ABSTRACT The effect of diabetes on trace elements concentration in blood of experimental animals has been studied by thin-target X-ray fluorescence analysis. Balb/C young adult mice, 6-8 wk old, were used in the study. About 100-200 I~L venous blood was taken from each mouse for trace element analysis. The measurements were carried out on a commercial Wave-Length-Dispersive XRF System, with different X-ray tubes being used for maximizing the detection sensitivity of different groups of elements. Later on, the mice were made diabetic by an intravenous injection of Streptozotocin (250 mg/kg). Then, 2 and 3 wk after the injection, 100 I~L of venous blood was drawn from each of the mice and analyzed for trace element concentration. In this way we were able to study the changes in blood trace elements caused by diabetes. The results and advantages of using experimental animals, under controlled conditions, to study trace element variations caused by different diseases, are discussed in the paper.
Biological Trace Element Research
429
Vol. 12, 1987
Effect of Diabetes on Trace-Element Concentration of Blood M. A. CHAUDHRI, 1 M. KOULMANDA, 2 M. T. HAUKKA, 3 AND N. NOORBAKHS j
IDepartment of Medical Physics, Austin Hospital, IVlelboume, and University of h'lelboume, Australia; 2Walter and Eliza Hall Institute of Medical Research, h'lelboume, Australia; and 3Department of Geology, University of Melbourne,/Vleiboume, Australia.
ABSTRACT The effect of diabetes on trace elements concentration in blood of experimental animals has been studied by thin-target X-ray fluorescence analysis. Balb/C young adult mice, 6-8 wk old, were used in the study. About 100-200 I~L venous blood was taken from each mouse for trace element analysis. The measurements were carried out on a commercial Wave-Length-Dispersive XRF System, with different X-ray tubes being used for maximizing the detection sensitivity of different groups of elements. Later on, the mice were made diabetic by an intravenous injection of Streptozotocin (250 mg/kg). Then, 2 and 3 wk after the injection, 100 I~L of venous blood was drawn from each of the mice and analyzed for trace element concentration. In this way we were able to study the changes in blood trace elements caused by diabetes. The results and advantages of using experimental animals, under controlled conditions, to study trace element variations caused by different diseases, are discussed in the paper.
Biological Trace Element Research
429
Vol. 12, 1987
