DOI 10.1515/cclm-2013-0705 Clin Chem Lab Med 2014; 52(5): 629–639
Johannes Zandera, Mathias Bruegela, Alisa Kleinhempel, Susen Becker, Sirak Petros, Linda Kortz, Juliane Dorow, Jürgen Kratzsch, Ronny Baber, Uta Ceglarek, Joachim Thiery and Daniel Teupser*
Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma Abstract
Johannes Zander and Mathias Bruegel contributed equally to this work. *Corresponding author: Daniel Teupser, Institute of Laboratory Medicine, Ludwig-Maximilians-University Munich, Marchioninistr. 15, 81377 Munich, Germany, Phone: +49 89 7095-3210, Fax: +49 89 7095-8888, E-mail: [email protected] Johannes Zander, Mathias Bruegel, Alisa Kleinhempel, Susen Becker, Linda Kortz, Juliane Dorow, Jürgen Kratzsch, Ronny Baber, Uta Ceglarek, Joachim Thiery and Daniel Teupser: Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Leipzig, Leipzig, Germany Johannes Zander, Susen Becker, Linda Kortz, Juliane Dorow, Ronny Baber, Uta Ceglarek, Joachim Thiery and Daniel Teupser: LIFE – Leipzig Research Center for Civilization Diseases, University Leipzig, Leipzig, Germany Johannes Zander, Mathias Bruegel, Alisa Kleinhempel and Daniel Teupser: Institute of Laboratory Medicine, Ludwig-MaximiliansUniversity Munich, Munich, Germany Sirak Petros: Medical ICU, University Leipzig, Leipzig, Germany
a
Background: Liquid biobanking is an important tool for laboratory diagnostics in routine settings and clinical studies. However, the current knowledge about adequate storage conditions for different classes of biomarkers is incomplete and, in part, contradictory. Here, we performed a comprehensive study on the effects of different storage conditions on the stability of various biomarkers in human serum and plasma. Methods: Serum and citrated plasma were aliquoted and stored at 4 °C, –20 °C, –80 °C, and
Johannes Zandera, Mathias Bruegela, Alisa Kleinhempel, Susen Becker, Sirak Petros, Linda Kortz, Juliane Dorow, Jürgen Kratzsch, Ronny Baber, Uta Ceglarek, Joachim Thiery and Daniel Teupser*
Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma Abstract
Johannes Zander and Mathias Bruegel contributed equally to this work. *Corresponding author: Daniel Teupser, Institute of Laboratory Medicine, Ludwig-Maximilians-University Munich, Marchioninistr. 15, 81377 Munich, Germany, Phone: +49 89 7095-3210, Fax: +49 89 7095-8888, E-mail: [email protected] Johannes Zander, Mathias Bruegel, Alisa Kleinhempel, Susen Becker, Linda Kortz, Juliane Dorow, Jürgen Kratzsch, Ronny Baber, Uta Ceglarek, Joachim Thiery and Daniel Teupser: Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Leipzig, Leipzig, Germany Johannes Zander, Susen Becker, Linda Kortz, Juliane Dorow, Ronny Baber, Uta Ceglarek, Joachim Thiery and Daniel Teupser: LIFE – Leipzig Research Center for Civilization Diseases, University Leipzig, Leipzig, Germany Johannes Zander, Mathias Bruegel, Alisa Kleinhempel and Daniel Teupser: Institute of Laboratory Medicine, Ludwig-MaximiliansUniversity Munich, Munich, Germany Sirak Petros: Medical ICU, University Leipzig, Leipzig, Germany
a
Background: Liquid biobanking is an important tool for laboratory diagnostics in routine settings and clinical studies. However, the current knowledge about adequate storage conditions for different classes of biomarkers is incomplete and, in part, contradictory. Here, we performed a comprehensive study on the effects of different storage conditions on the stability of various biomarkers in human serum and plasma. Methods: Serum and citrated plasma were aliquoted and stored at 4 °C, –20 °C, –80 °C, and
